Gpr4 (NM_175668) Mouse Tagged ORF Clone
CAT#: MR205619
- TrueORF®
Gpr4 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 4 (Gpr4)
ORF Plasmid: tGFP
"NM_175668" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205619 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACAGCACGGGCACAGGGGAGGGCTGCCATGTGGACTCTCGAGTGGACCACCTCTTCCCACCAT CTCTCTACATCTTCGTCATCGGGGTGGGGCTGCCCACCAACTGCCTGGCCCTGTGGGCAGCCTACCGGCA GGTGCGCCAACACAATGAGCTGGGCGTCTACCTGATGAACTTGAGCATTGCAGACCTGCTGTACATCTGC ACTTTGCCGCTGTGGGTCGACTACTTCCTCCACCATGACAACTGGATCCACGGCCCTGGCTCCTGCAAGC TCTTTGGCTTCATCTTCTACAGCAACATCTATATCAGCATCGCCTTCCTGTGCTGCATCTCCGTGGACCG CTACCTGGCTGTGGCTCATCCTCTGCGCTTTGCACGCCTGCGCCGGGTCAAGACAGCAGTGGCTGTGAGC TCTGTGGTCTGGGCCACGGAGCTGGGCGCCAATTCAGCACCGCTCTTCCATGATGAGCTGTTTCGTGATC GCTACAACCACACCTTCTGCTTTGAGAAGTTCCCCATGGAGCGTTGGGTGGCCTGGATGAATCTGTACCG CGTCTTTGTGGGCTTCCTCTTCCCCTGGGCACTCATGTTGCTGTGCTACCGTGGCATCCTGAGGGCAGTG CAGAGCAGTGTGTCCACCGAGCGCCAGGAGAAAGTCAAGATCAAACGTCTGGCCCTGAGCCTCATCGCCA TTGTGCTGGTGTGCTTTGCGCCTTACCATGCTCTCCTGCTGTCTCGCAGCGCCGTCTACCTGGGCCGGCC CTGGGACTGTGGCTTCGAGGAGCGAGTCTTTTCTGCCTACCACAGCTCCCTGGCCTTCACCAGCCTCAAT TGTGTGGCTGACCCCATCCTCTACTGCCTGGTCAACGAGGGTGCCCGCAGTGATGTGGCCAAGGCCCTGC ACAACCTCCTCCGCTTCCTGGCCAGCAACAAGCCCCAGGAGATGGCCAATGCTTCCCTCACCCTGGAGAC ACCCTTGACCTCCAAGAGGAGCACCACCGGCAAGTCGTCCGGGGCTGTCTGGGCAGTGCCTCCGACTGCC CAGGGGGACCAGGTGCCACTGAAGGTGCTGCTGCCCCCGGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205619 protein sequence
Red=Cloning site Green=Tags(s) MDNSTGTGEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVRQHNELGVYLMNLSIADLLYIC TLPLWVDYFLHHDNWIHGPGSCKLFGFIFYSNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVS SVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMERWVAWMNLYRVFVGFLFPWALMLLCYRGILRAV QSSVSTERQEKVKIKRLALSLIAIVLVCFAPYHALLLSRSAVYLGRPWDCGFEERVFSAYHSSLAFTSLN CVADPILYCLVNEGARSDVAKALHNLLRFLASNKPQEMANASLTLETPLTSKRSTTGKSSGAVWAVPPTA QGDQVPLKVLLPPAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_175668 |
ORF Size | 1098 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175668.4, NP_783599.1 |
RefSeq Size | 2884 bp |
RefSeq ORF | 1098 bp |
Locus ID | 319197 |
UniProt ID | Q8BUD0 |
MW | 41.1 kDa |
Gene Summary | Proton-sensing receptor coupled to several G-proteins, including G(s), G(13) and G(q)/G(11) proteins, leading to cAMP production.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207953 | Gpr4 (untagged) - Mouse G protein-coupled receptor 4 (Gpr4), (10ug) |
CNY 3,656.00 |
|
MG205619 | Gpr4 (tGFP-tagged) - Mouse G protein-coupled receptor 4 (Gpr4) |
CNY 3,140.00 |
|
MR205619L3 | Lenti ORF clone of Gpr4 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 4 (Gpr4) |
CNY 4,750.00 |
|
MR205619L4 | Lenti ORF clone of Gpr4 (mGFP-tagged) - Mouse G protein-coupled receptor 4 (Gpr4) |
CNY 4,750.00 |