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Gna13 (NM_010303) Mouse Tagged ORF Clone
CAT＃: MR205863
- TrueORF®
Gna13 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha 13 (Gna13)
ORF Plasmid: tGFP
"NM_010303" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU024132; AU043124; Galpha13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205863 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACTTCCTGCCGTCGCGCTCCGTGCTGTCCGTGTGCTTCCCGGGCTGCGTGCTGACGAACGGCG AGGCCGAGCAGCAGCGCAAGTCCAAGGAGATCGACAAATGCCTGTCGCGGGAGAAGACCTACGTGAAGCG GCTGGTGAAGATCCCGCTGCTGGGCGCGGGCGAGAGCGGCAAGTCCACCTTCCTGAAGCAGATGCGGATC ATCCACGGCCAGGACTTCGACCAGCGCGCGCGCGAGGAGTTCCGCCCCACCATCTACAGCAACGTGATCA AAGGTATGAGGGTGCTGGTAGATGCCCGAGAGAAGCTTCATATTCCCTGGGGAGATAACAAAAACCAGCT CCATGGAGACAAGTTGATGGCATTTGATACCCGCGCCCCCATGGCTGCCCAGGGGATGGTGGAGACTCGA GTGTTCCTGCAGTATCTTCCTGCTATCAGAGCCTTATGGGAGGACAGTGGTATACAGAATGCCTACGATC GGCGCCGGGAATTCCAGCTGGGTGAGTCTGTAAAGTATTTCTTGGATAACTTGGATAAACTTGGAGTACC GGATTACATTCCATCACAGCAAGATATCCTGCTTGCCAGAAGGCCCACCAAAGGCATCCATGAGTACGAC TTTGAAATTAAAAATGTTCCTTTCAAAATGGTTGATGTAGGTGGCCAGAGATCAGAACGGAAACGCTGGT TTGAATGCTTTGACAGTGTGACGTCGATACTTTTCCTTGTCTCTTCAAGTGAATTTGACCAGGTGCTTAT GGAGGACCGCCAGACCAATCGCCTTACAGAATCTCTGAACATTTTTGAAACAATTGTCAACAATCGGGTT TTCAGCAACGTCTCCATAATCCTCTTCTTAAACAAGACAGACTTGCTCGAGGAGAAAGTGCAAGTTGTTA GCATCAAAGACTATTTCCTAGAATTTGAAGGGGACCCCCACTGCTTAAGAGACGTCCAAAAGTTTCTGGT GGAATGCTTCCGGGGGAAACGCCGGGACCAGCAGCAGAGGCCGTTGTACCACCACTTCACCACCGCGATC AACACAGAGAACATCCGCCTCGTGTTCCGGGACGTGAAGGACACGATCCTTCATGACAACCTGAAGCAGC TCATGCTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205863 protein sequence
Red=Cloning site Green=Tags(s) MADFLPSRSVLSVCFPGCVLTNGEAEQQRKSKEIDKCLSREKTYVKRLVKIPLLGAGESGKSTFLKQMRI IHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNKNQLHGDKLMAFDTRAPMAAQGMVETR VFLQYLPAIRALWEDSGIQNAYDRRREFQLGESVKYFLDNLDKLGVPDYIPSQQDILLARRPTKGIHEYD FEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRQTNRLTESLNIFETIVNNRV FSNVSIILFLNKTDLLEEKVQVVSIKDYFLEFEGDPHCLRDVQKFLVECFRGKRRDQQQRPLYHHFTTAI NTENIRLVFRDVKDTILHDNLKQLMLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010303 |
| ORF Size | 1134 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010303.2 |
| RefSeq Size | 6217 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 14674 |
| UniProt ID | P27601 |
| MW | 44 kDa |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:21212405, PubMed:19151758, PubMed:16388592). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:16388592). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (PubMed:19151758, PubMed:21212405). Promotes tumor cell invasion and metastasis by activating Rho/ROCK signaling pathway (By similarity). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205559 | Gna13 (untagged) - Mouse guanine nucleotide binding protein, alpha 13 (Gna13), (10ug) |
CNY 3,656.00 |
|
| MG205863 | Gna13 (tGFP-tagged) - Mouse guanine nucleotide binding protein, alpha 13 (Gna13) |
CNY 5,256.00 |
|
| MR205863L3 | Lenti ORF clone of Gna13 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha 13 (Gna13) |
CNY 4,750.00 |
|
| MR205863L4 | Lenti ORF clone of Gna13 (mGFP-tagged) - Mouse guanine nucleotide binding protein, alpha 13 (Gna13) |
CNY 4,750.00 |
