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Stn1 (NM_175360) Mouse Tagged ORF Clone
CAT＃: MR205901
- TrueORF®
Obfc1 (Myc-DDK-tagged) - Mouse oligonucleotide/oligosaccharide-binding fold containing 1 (Obfc1)
ORF Plasmid: tGFP
"NM_175360" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610009H20Rik; 2310057J23Rik; AAF-44; AAF44; AI413458; Obfc1; RPA-32 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205901 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGTGAATCCAGTCCAAGGGAAGAGGAGATCCCGCCCCTCTTTTGGGGTCTGGATCCTGTGTTTC TAGCCTTTGCAAAACTCTACATCAAGGATATCCTGGAGATGAAGGAGTCCCAACAAGTGCCAGGTACATA TTTTTACAACGGGCATCCAATAAGGCGGGTAGATATCATGGGAGCTGTCATCAGCGTGAAAGAAAGAGAG ACTTTCTACAGCTACGGAGTGGACGATGCCACTGGGGTTATAAACTGCGTCTGCTGGAAGAAGCTGAGCA ATGCTGAGTCTTCCTCAGACCCAGCTATTCTGAGCACTGCGAGAGAGCTTAGCATGACATCACAGCTTAA GAAGCTGCAGGAGACCATCGAGCAGAAAACCAGGATAGGGATTGGAGATATCATCCGAGTCCGAGGCTCT GTGCGCATGTTCCGAGAAGAGCGAGAGATCTGTGCCAACATTTATTATAAGGTGGACGATCCAGTGTGGA ACATGCAAATCGCAAGGATGCTTGAGCTGCCCAAGCTCTACCAGAAGGTGTACGACCAGCCCTTCCGTAA CCCAGCCCTACAGGAGGAGGAAGCACTAAACAATAAAGACAATTTGGATCTCGCTGGCCTCACATCTTTG CTGAGTGAGAAAATTAAAGAATTCCTCCAGGAGAAGAAAATGCAAAGCTTCTACCAGCAGGAGTTAGAGA CAGTGGAGTCTTTGCAGTCTCTGGCCAGTCGGCCTGTGACCCACAGCACGGGCTCGGACCAAGTGGAGTT AAAGGACAGTGGCACTTCTGGCGTAGCGCAGAGAGTGTTTAAGAATGCTCTCCAGCTGCTTCAGGAAAAA GGACTCGTGTTCCAGAGAGACAGTGGCTCTGATAAGCTGTACTACGTAACCACCAAAGACAAAGACCTGC AGCAGAAGATCTACCACATCATTAAGGAAGACTGTCAGAAACCAAACCATATGGAGAAGGGCTGCCACCT GCTACACATCCTGAAGTGCGTGCACCTCAACCTCCGCTGGGACCTGAGCAAGGCTGTGCTGCAGCGGGTG CTGGAGCTCCTGGAGGACCAGAGTGACATCGTCAGCACCGCGGACCACTACTACGCGGCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205901 protein sequence
Red=Cloning site Green=Tags(s) MECESSPREEEIPPLFWGLDPVFLAFAKLYIKDILEMKESQQVPGTYFYNGHPIRRVDIMGAVISVKERE TFYSYGVDDATGVINCVCWKKLSNAESSSDPAILSTARELSMTSQLKKLQETIEQKTRIGIGDIIRVRGS VRMFREEREICANIYYKVDDPVWNMQIARMLELPKLYQKVYDQPFRNPALQEEEALNNKDNLDLAGLTSL LSEKIKEFLQEKKMQSFYQQELETVESLQSLASRPVTHSTGSDQVELKDSGTSGVAQRVFKNALQLLQEK GLVFQRDSGSDKLYYVTTKDKDLQQKIYHIIKEDCQKPNHMEKGCHLLHILKCVHLNLRWDLSKAVLQRV LELLEDQSDIVSTADHYYAAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175360 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175360.1, NM_175360.2, NP_780569.1 |
| RefSeq Size | 1878 bp |
| RefSeq ORF | 1137 bp |
| Locus ID | 108689 |
| UniProt ID | Q8K2X3 |
| MW | 42.7 kDa |
| Gene Summary | Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22748632). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205841 | Obfc1 (untagged) - Mouse oligonucleotide/oligosaccharide-binding fold containing 1 (Obfc1), (10ug) |
CNY 3,656.00 |
|
| MG205901 | Obfc1 (tGFP-tagged) - Mouse oligonucleotide/oligosaccharide-binding fold containing 1 (Obfc1) |
CNY 3,140.00 |
|
| MR205901L3 | Lenti ORF clone of Obfc1 (Myc-DDK-tagged) - Mouse oligonucleotide/oligosaccharide-binding fold containing 1 (Obfc1) |
CNY 4,750.00 |
|
| MR205901L4 | Lenti ORF clone of Obfc1 (mGFP-tagged) - Mouse oligonucleotide/oligosaccharide-binding fold containing 1 (Obfc1) |
CNY 4,750.00 |
