Cd55 (NM_010016) Mouse Tagged ORF Clone
CAT#: MR206092
- TrueORF®
Cd55 (Myc-DDK-tagged) - Mouse CD55 antigen (Cd55)
ORF Plasmid: tGFP
"NM_010016" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Daf; Daf-; Daf-GPI; Daf1; GPI-; GPI-DAF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206092 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGTGGGCGGGCGCCTAGGACTCGGCCATCACCGCCGCCTCCGCTGCTGCCGTTGCTGTCGCTGT CTCTGTTGCTGCTGTCCCCAACTGTACGCGGAGACTGCGGCCCACCTCCAGACATTCCTAATGCCAGGCC AATCTTGGGCAGACACTCCAAGTTTGCTGAGCAAAGCAAAGTGGCATACTCGTGTAATAACGGCTTTAAA CAAGTTCCAGACAAGTCAAACATAGTTGTCTGTCTTGAAAATGGCCAATGGTCGAGCCACGAAACATTCT GTGAGAAATCACTTGTTGCTCCAGAAAGACTGAGTTTTGCATCCCTCAAAAAAGAGTACCTCCACATGAA TTTTTTCCCAGTTGGTACTATTGTGGAATATGAGTGTCGGCCAGGATTTCGAAAACAACCTCCACTCCCA GGAAAATCAACTTGCCTTGAGGATTTAGTATGGTCTCCAGTTGCTCAGTTTTGTAAAAAAAAATCATGCC CTAATCCTAAAGATCTGGATAATGGTCACATCAACATACCAACCGGCATATTATTCGGTTCAGAAATAAA CTTCTCATGCAACCCAGGGTACAGGCTAGTTGGTGTCTCCTCTACTTTCTGTTCTGTCACAGGAAATACT GTTGATTGGGACGATGAGTTTCCAGTGTGCACAGAAATACATTGTCCAGAGCCACCAAAAATCAACAATG GAATAATGCGAGGGGAAAGTGACTCTTATACGTATAGCCAGGTGGTCACCTATTCATGTGACAAAGGCTT CGTCCTGGTTGGAAATGCTAGCATTTATTGTACTGTGAGCAAGTCTGATGTAGGACAATGGAGCAGTCCA CCACCCCGGTGCATAGAGAAATCCAAGGTCCCAACGAAGAAACCAACAATTAATGTTCCAAGTACAGGAA CCCCCTCAACGCCTCAGAAACCCACACTAGAAAGTGTTCCAAATCCAGGAGACCAACCAACTCCTCAGAA ACCTTCCACAGTTAAAGTTTCAGCAACCCAGCATGTACCTGTTACCAAGACAACAGTACGTCATCCAATA AGAACATCTACAGACAAAGGAGAGCCTAACACAGGTGGTGACCGTTATATATATGGACATACATGTTTAA TAACCTTGACAGTTTTGCATGCGATGCTATCACTCATTGGCTACTTGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206092 protein sequence
Red=Cloning site Green=Tags(s) MIRGRAPRTRPSPPPPLLPLLSLSLLLLSPTVRGDCGPPPDIPNARPILGRHSKFAEQSKVAYSCNNGFK QVPDKSNIVVCLENGQWSSHETFCEKSLVAPERLSFASLKKEYLHMNFFPVGTIVEYECRPGFRKQPPLP GKSTCLEDLVWSPVAQFCKKKSCPNPKDLDNGHINIPTGILFGSEINFSCNPGYRLVGVSSTFCSVTGNT VDWDDEFPVCTEIHCPEPPKINNGIMRGESDSYTYSQVVTYSCDKGFVLVGNASIYCTVSKSDVGQWSSP PPRCIEKSKVPTKKPTINVPSTGTPSTPQKPTLESVPNPGDQPTPQKPSTVKVSATQHVPVTKTTVRHPI RTSTDKGEPNTGGDRYIYGHTCLITLTVLHAMLSLIGYLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010016 |
ORF Size | 1173 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010016.1, NM_010016.2, NM_010016.3, NP_034146.2 |
RefSeq Size | 2527 bp |
RefSeq ORF | 1173 bp |
Locus ID | 13136 |
UniProt ID | Q61475 |
MW | 42.6 kDa |
Gene Summary | This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200849 | Cd55 (untagged) - Mouse CD55 antigen (Cd55), (10ug) |
CNY 3,656.00 |
|
MG206092 | Cd55 (tGFP-tagged) - Mouse CD55 antigen (Cd55) |
CNY 3,710.00 |
|
MR206092L3 | Lenti ORF clone of Cd55 (Myc-DDK-tagged) - Mouse CD55 antigen (Cd55) |
CNY 5,230.00 |
|
MR206092L4 | Lenti ORF clone of Cd55 (mGFP-tagged) - Mouse CD55 antigen (Cd55) |
CNY 5,230.00 |