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Ugcg (NM_011673) Mouse Tagged ORF Clone
CAT＃: MR206157
- TrueORF®
Ugcg (Myc-DDK-tagged) - Mouse UDP-glucose ceramide glucosyltransferase (Ugcg)
ORF Plasmid: tGFP
"NM_011673" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU043821; C80537; Epcs21; GlcT-1; Ugcgl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206157 representing NM_011673
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCTGGACCTGGCCCAGGAGGGAATGGCCTTGTTCGGCTTCGTGCTCTTCGTGGTGCTGTGGC TGATGCATTTCATGTCCATCATCTACACCCGGTTACACCTCAACAAGAAGGCAACAGACAAACAGCCGTA TAGCAAGCTCCCTGGTGTCTCTCTTCTGAAGCCACTGAAGGGGGTGGATCCTAACCTAATCAACAACTTG GAGACATTCTTTGAACTGGATTATCCCAAATATGAAGTACTCCTTTGTGTACAAGATCATGATGATCCAG CCATTGATGTATGTAAGAAATTGCTTGGAAAATACCCAAATGTCGATGCTAGATTATTTATAGGTGGCAA AAAGGTTGGCATTAACCCTAAAATTAATAATTTGATGCCAGCATATGAAGTTGCAAAATATGATCTCATA TGGATTTGTGATAGCGGAATAAGAGTCATCCCAGACACATTAACTGACATGGTGAATCAGATGACAGAGA AAGTGGGGTTGGTCCACGGGCTGCCGTATGTAGCCGACAGACAAGGCTTTGCTGCCACCTTAGAGCAGGT ATATTTTGGAACTTCACACCCAAGATCCTATATCTCTGCCAATGTAACTGGCTTCAAATGTGTGACGGGG ATGTCTTGTTTGATGAGGAAGGATGTGCTAGATCAGGCAGGAGGGCTCATAGCCTTTGCTCAGTACATTG CTGAAGATTACTTTATGGCCAAAGCAATAGCCGACCGAGGTTGGAGGTTTTCAATGTCTACTCAAGTTGC CATGCAAAACTCTGGTTCGTACTCAATTTCTCAGTTTCAATCCAGAATGATCAGGTGGACCAAATTGAGA ATTAACATGCTTCCTGCTACAATAATTTGTGAGCCAATTTCAGAATGCTTTGTTGCCAGTTTAATTATTG GGTGGGCAGCCCACCATGTATTCAGATGGGATATCATGGTCTTCTTCATGTGCCACTGCCTGGCATGGTT TATATTTGACTACATTCAACTCAGGGGTGTCCAGGGTGGCACACTGTGTTTTTCAAAACTTGATTATGCT GTGGCCTGGTTCATCCGTGAATCCATGACAATCTACATTTTCCTGTCGGCATTATGGGACCCGACTATAA GCTGGAGAACTGGTCGCTACAGGTTACGCTGTGGGGGGACAGCAGAGGAGATCCTGGATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206157 representing NM_011673
Red=Cloning site Green=Tags(s) MALLDLAQEGMALFGFVLFVVLWLMHFMSIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNL ETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPAYEVAKYDLI WICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRSYISANVTGFKCVTG MSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFSMSTQVAMQNSGSYSISQFQSRMIRWTKLR INMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYA VAWFIRESMTIYIFLSALWDPTISWRTGRYRLRCGGTAEEILDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011673 |
| ORF Size | 1182 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011673.2 |
| RefSeq Size | 3719 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 22234 |
| UniProt ID | O88693 |
| MW | 45.3 kDa |
| Gene Summary | Catalyzes at the cytosolic surface of the Golgi, the initial step of the glucosylceramide-based glycosphingolipid/GSL synthetic pathway, the transfer of glucose from UDP-glucose to ceramide to produce glucosylceramide/GlcCer (PubMed:10430909, PubMed:16109770, PubMed:28373486). Glucosylceramide is the core component of glycosphingolipids/GSLs, amphipathic molecules consisting of a ceramide lipid moiety embedded in the outer leaflet of the membrane, linked to one of hundreds of different externally oriented oligosaccharide structures (PubMed:10430909). Glycosphingolipids are essential components of membrane microdomains that mediate membrane trafficking and signal transduction (PubMed:10430909). They are implicated in many fundamental cellular processes, including growth, differentiation, migration, morphogenesis, cell-to-cell and cell-to-matrix interactions (PubMed:10430909). They are required for instance in the proper development and functioning of the nervous system (PubMed:16109770). As an example of their role in signal transduction, they regulate the leptin receptor/LEPR in the leptin-mediated signaling pathway (PubMed:23554574). They also play an important role in the establishment of the skin barrier regulating keratinocyte differentiation and the proper assembly of the cornified envelope (PubMed:17145749, PubMed:23748427). The biosynthesis of GSLs is also required for the proper intestinal endocytic uptake of nutritional lipids (PubMed:22851168).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209618 | Ugcg (untagged) - Mouse UDP-glucose ceramide glucosyltransferase (Ugcg), (10ug) |
CNY 5,488.00 |
|
| MG206157 | Ugcg (tGFP-tagged) - Mouse UDP-glucose ceramide glucosyltransferase (Ugcg) |
CNY 5,256.00 |
|
| MR206157L3 | Lenti ORF clone of Ugcg (Myc-DDK-tagged) - Mouse UDP-glucose ceramide glucosyltransferase (Ugcg) |
CNY 6,056.00 |
|
| MR206157L4 | Lenti ORF clone of Ugcg (mGFP-tagged) - Mouse UDP-glucose ceramide glucosyltransferase (Ugcg) |
CNY 5,890.00 |
