Pten (NM_008960) Mouse Tagged ORF Clone
CAT#: MR206321
- TrueORF®
Pten (Myc-DDK-tagged) - Mouse phosphatase and tensin homolog (Pten)
ORF Plasmid: tGFP
"NM_008960" in other vectors (5)
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CNY 5,488.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; MMAC; MMAC1; PTENbeta; TEP; TEP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206321 representing NM_008960
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAG ACTTGACCTATATTTATCCAAATATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGTGTATACAG GAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATACAATCTA TGTGCTGAGAGACATTATGACACCGCCAAATTTAACTGCAGAGTTGCACAGTATCCTTTTGAAGACCATA ACCCACCACAGCTAGAACTTATCAAACCCTTCTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAA TCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGGACTGGTGTAATGATTTGTGCATATTTATTG CATCGGGGCAAATTTTTAAAGGCACAAGAGGCCCTAGATTTTTATGGGGAAGTAAGGACCAGAGACAAAA AGGGAGTCACAATTCCCAGTCAGAGGCGCTATGTATATTATTATAGCTACCTGCTAAAAAATCACCTGGA TTACAGACCCGTGGCACTGCTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACT TGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCACGCGGC GGGAGGACAAGTTCATGTACTTTGAGTTCCCTCAGCCATTGCCTGTGTGTGGTGATATCAAAGTAGAGTT CTTCCACAAACAGAACAAGATGCTCAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATA CCAGGACCAGAGGAAACCTCAGAAAAAGTGGAAAATGGAAGTCTTTGTGATCAGGAAATCGATAGCATTT GCAGTATAGAGCGTGCAGATAATGACAAGGAGTATCTTGTACTCACCCTAACAAAAAACGATCTTGACAA AGCAAACAAAGACAAGGCCAACCGATACTTCTCTCCAAATTTTAAGGTGAAACTATACTTTACAAAAACA GTAGAGGAGCCATCAAATCCAGAGGCTAGCAGTTCAACTTCTGTGACTCCAGATGTTAGTGACAATGAAC CTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCA TTCACAAATTACAAAAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206321 representing NM_008960
Red=Cloning site Green=Tags(s) MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNL CAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLL HRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGT CNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFI PGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKT VEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008960 |
ORF Size | 1209 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008960.2, NP_032986.1 |
RefSeq Size | 8229 bp |
RefSeq ORF | 1212 bp |
Locus ID | 19211 |
UniProt ID | O08586 |
MW | 47.6 kDa |
Gene Summary | This gene encodes a phosphatase with dual activity against phospholipids and proteins, and acts as a tumor-suppressor. The protein contains four structural domains, a PIP2-binding domain, a catalytic tensin-type phosphatase domain, a C2 tensin-type domain and a PDZ-binding domain. The protein belongs to the protein tyrosine phosphatase family. Deletion of this gene in mice contribute to tumorigenesis in multiple tissues. [provided by RefSeq, Sep 2015] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Cerebellar Ganglioglioma in Childhood: Histopathologic Implications for Management During Long-term Survival: A Case Report
,null,
Yonago Acta Medica
,PubMed ID 29434497
[Pten]
|
Mutation in the FGFR1 tyrosine kinase domain or inactivation of PTEN is associated with acquired resistance to FGFR inhibitors in FGFR1-driven leukemia/lymphomas
,null,
International journal of cancer
,PubMed ID 28646488
[Pten]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG206321 | Pten (tGFP-tagged) - Mouse phosphatase and tensin homolog (Pten) |
CNY 7,088.00 |
|
MR206321L1 | Lenti ORF clone of Pten (Myc-DDK-tagged) - Mouse phosphatase and tensin homolog (Pten) |
CNY 7,888.00 |
|
MR206321L2 | Lenti ORF clone of Pten (mGFP-tagged) - Mouse phosphatase and tensin homolog (Pten) |
CNY 5,890.00 |
|
MR206321L3 | Lenti ORF clone of Pten (Myc-DDK-tagged) - Mouse phosphatase and tensin homolog (Pten) |
CNY 7,888.00 |
|
MR206321L4 | Lenti ORF clone of Pten (mGFP-tagged) - Mouse phosphatase and tensin homolog (Pten) |
CNY 7,888.00 |