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Doc2a (NM_010069) Mouse Tagged ORF Clone
CAT＃: MR206372
- TrueORF®
Doc2a (Myc-DDK-tagged) - Mouse double C2, alpha (Doc2a)
ORF Plasmid: tGFP
"NM_010069" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206372 representing NM_010069
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGAGGGGCCGCAGGGGCGATCGCATGACCATCAACATCCAGGAGCACATGGCCATCAACGTGTGCCCTG GACCCATCAGGCCCATCCGCCAGATCTCCGACTACTTCCCTCGCAGGGGGCCAGGACCAGAGGGTGGCGG CGGCGGCGGTGGCACGGGCTGCGGGGAAGCCCCAGCTCATCTGGCCCCTCTGGCTCTGGCCCCCCCTGCG GCTCTCCTTGGGGCCACTACACCCGACGATGGAGCTGAGGTAGACAGCTACGACTCGGATGATACCACCG CCCTGGGCACACTGGAATTTGACCTTCTCTATGATCAGGCTTCCTGCATGCTGCACTGTAGAATCCTCAG GGCCAAGGGCCTCAAGCCCATGGATTTCAATGGCCTGGCTGACCCCTATGTAAAGCTTCACCTCCTGCCA GGAGCCTGCAAGGCCAATAAGCTAAAAACCAAGACACAGAGGAACACACTGAACCCTGTGTGGAATGAGG AGCTGACGTACAGCGGGATCACGGATGATGACATCACCCACAAGGTGCTCAGGATCTCTGTCTGTGATGA GGACAAGCTGAGCCACAATGAATTCATTGGGGAGATCCGAGTGCCCCTCCGCCGCCTCAAGCCTTCACAG AAGAAGCATTTTAACATCTGCCTTGAGCGCCAGGTCCCGCTTCCTTCACCCTCTTCAATGTCTGCGGCGC TGAGGGGCATATCCTGTTACCTGAAGGAGCTGGAGCAGGCAGAGCAGGGACCTGGGCTGCTGGAAGAGCG CGGGCGCATCCTGCTGAGCCTCAGCTACAGCTCTCGGCGGCATGGGCTGCTGGTGGGCATTGTTCGCTGT GCGCACCTGGCTGCAATGGATGTTAACGGCTACTCTGACCCTTATGTGAAGACGTACTTGAGACCAGATG TGGATAAGAAATCCAAGCACAAAACATGTGTAAAGAAGAAGACACTAAATCCGGAATTTAATGAGGAATT CTTCTATGAGATTGAACTCTCCACTCTGGCCACTAAGACCCTGGAGGTCACAGTCTGGGACTACGACATT GGCAAATCCAATGACTTCATAGGTGGTGTGTCTCTGGGGCCAGGAGCCCGGGGAGAGGCCCAGAAACACT GGAATGACTGTCTACATCAGCCGGACACAGCCCTGGAGCGCTGGCATACTCTGACCAGCGAGCTGCCCCC TGCAGCAGGGGCTTACCCTTTGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206372 representing NM_010069
Red=Cloning site Green=Tags(s) MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRRGPGPEGGGGGGGTGCGEAPAHLAPLALAPPA ALLGATTPDDGAEVDSYDSDDTTALGTLEFDLLYDQASCMLHCRILRAKGLKPMDFNGLADPYVKLHLLP GACKANKLKTKTQRNTLNPVWNEELTYSGITDDDITHKVLRISVCDEDKLSHNEFIGEIRVPLRRLKPSQ KKHFNICLERQVPLPSPSSMSAALRGISCYLKELEQAEQGPGLLEERGRILLSLSYSSRRHGLLVGIVRC AHLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELSTLATKTLEVTVWDYDI GKSNDFIGGVSLGPGARGEAQKHWNDCLHQPDTALERWHTLTSELPPAAGAYPLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010069 |
| ORF Size | 1215 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010069.1, NP_034199.1 |
| RefSeq Size | 2557 bp |
| RefSeq ORF | 1218 bp |
| Locus ID | 13446 |
| UniProt ID | Q7TNF0 |
| MW | 45.1 kDa |
| Gene Summary | Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205087 | Doc2a (untagged) - Mouse double C2, alpha (Doc2a), (10ug) |
CNY 3,656.00 |
|
| MG206372 | Doc2a (tGFP-tagged) - Mouse double C2, alpha (Doc2a) |
CNY 3,140.00 |
|
| MR206372L3 | Lenti ORF clone of Doc2a (Myc-DDK-tagged) - Mouse double C2, alpha (Doc2a) |
CNY 4,750.00 |
|
| MR206372L4 | Lenti ORF clone of Doc2a (mGFP-tagged) - Mouse double C2, alpha (Doc2a) |
CNY 4,750.00 |
