Ass1 (NM_007494) Mouse Tagged ORF Clone
CAT#: MR206472
- TrueORF®
Ass1 (Myc-DDK-tagged) - Mouse argininosuccinate synthetase 1 (Ass1)
"NM_007494" in other vectors (3)
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CNY 3,656.00
CNY 4,090.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA408052; ASS; Ass-1; fold |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206472 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCAAGGGCTCTGTGGTTCTGGCCTACAGTGGTGGCCTGGACACCTCCTGCATCCTCGTGTGGC TGAAGGAACAAGGCTATGATGTCATCGCCTACCTGGCCAACATTGGCCAGAAGGAAGACTTTGAGGAAGC CAGGAAGAAGGCGCTGAAGCTTGGGGCCAAAAAGGTGTTCATTGAGGATGTGAGCAAGGAATTTGTGGAA GAGTTCATCTGGCCTGCTGTCCAGTCCAGTGCACTCTACGAGGACCGCTATCTCCTGGGCACCTCTCTCG CCAGGCCTTGCATAGCTCGCAGACAGGTGGAGATTGCCCAGCGTGAAGGGGCCAAGTATGTGTCTCACGG CGCCACGGGAAAGGGGAATGACCAGGTCCGCTTTGAGCTCACCTGCTACTCACTGGCACCCCAGATTAAG GTCATCGCTCCCTGGAGGATGCCTGAGTTTTACAACCGGTTCAAGGGCCGAAATGATCTGATGGAGTATG CAAAGCAACACGGAATCCCCATCCCTGTCACCCCCAAGAGCCCCTGGAGTATGGATGAAAACCTCATGCA CATCAGCTATGAGGCTGGGATCCTGGAAAACCCCAAGAATCAAGCACCTCCGGGTCTCTACACAAAAACT CAGGACCCTGCCAAAGCACCCAACAGCCCAGATGTCCTTGAGATAGAATTCAAAAAAGGGGTCCCTGTGA AGGTGACCAACATCAAAGATGGCACAACCCGCACCACATCCCTGGAACTCTTCATGTACCTGAACGAAGT TGCGGGCAAGCACGGAGTGGGTCGCATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT ATCTACGAGACCCCAGCAGGGACCATCCTTTACCACGCTCATTTAGACATAGAGGCCTTCACGATGGATC GGGAAGTACGCAAAATCAAGCAGGGCCTGGGCCTCAAATTCGCAGAGCTCGTATACACAGGTTTCTGGCA CAGCCCTGAATGTGAATTTGTTCGCCACTGTATCCAGAAGTCCCAGGAGCGGGTAGAAGGGAAGGTGCAG GTGTCTGTCTTCAAGGGCCAAGTGTACATCCTCGGTCGGGAGTCTCCACTTTCACTCTACAATGAAGAGC TGGTGAGCATGAACGTACAGGGCGACTATGAGCCCATCGACGCCACTGGCTTCATCAATATCAACTCGCT CAGGCTGAAGGAGTACCATCGCCTTCAGAGCAAGGTCACTGCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206472 protein sequence
Red=Cloning site Green=Tags(s) MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVE EFIWPAVQSSALYEDRYLLGTSLARPCIARRQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIK VIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKT QDPAKAPNSPDVLEIEFKKGVPVKVTNIKDGTTRTTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRG IYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIQKSQERVEGKVQ VSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007494 |
ORF Size | 1239 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007494.2 |
RefSeq Size | 1631 bp |
RefSeq ORF | 1239 bp |
Locus ID | 11898 |
UniProt ID | P16460 |
MW | 46.6 kDa |
Gene Summary | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Therapeutic targeting of argininosuccinate synthase 1 (ASS1)-deficient pulmonary fibrosis
,Li, JM;Yang, DC;Oldham, J;Linderholm, A;Zhang, J;Liu, J;Kenyon, NJ;Chen, CH;,
Molecular therapy : the journal of the American Society of Gene Therapy
,PubMed ID 33508432
[ASS1]
|
Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM
,Przystal, JM;Hajji, N;Khozoie, C;Renziehausen, A;Zeng, Q;Abaitua, F;Hajitou, A;Suwan, K;Want, E;Bomalaski, J;Szlosarek, P;O'Neill, K;Crook, T;Syed, N;,
Cell Death Dis
,PubMed ID 30546006
[ASS1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207991 | Ass1 (untagged) - Mouse argininosuccinate synthetase 1 (Ass1), (10ug) |
CNY 5,488.00 |
|
MR206472L3 | Lenti ORF clone of Ass1 (Myc-DDK-tagged) - Mouse argininosuccinate synthetase 1 (Ass1) |
CNY 5,990.00 |
|
MR206472L4 | Lenti ORF clone of Ass1 (mGFP-tagged) - Mouse argininosuccinate synthetase 1 (Ass1) |
CNY 6,056.00 |