Trmu (NM_028063) Mouse Tagged ORF Clone
CAT#: MR206594
- TrueORF®
Trmu (Myc-DDK-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_028063" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110005N20Rik; 1600025P05Rik; AI314320; Trmt1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206594 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGCTGCGGCACGTCGTGTGCGCCCTGTCTGGCGGTGTGGACAGCGCGGTGGCGGCGCTGCTGT TGCGGCGGAGAGGTTACCAGGTGACAGGGGTATTTATGAAGAACTGGGACTCGCTGGATGAGCAGGGCGT TTGTGCTGCTGACAAGGACTGTGAAGATGCGTATAAAGTTTGTCAGATCTTAGACATCCCTTTCCACCAG GTGTCCTATGTGAAGGAGTATTGGAATGATGTGTTCAGTGACTTTTTGAACGAGTATGAGAAAGGAAGGA CTCCAAACCCCGACATCAACTGCAATAAGCATATCAAGTTCAGTTGCTTTTATCACTATGCTGTGGACAA TCTTGGAGCAGATGCCGTTGCCACTGGCCACTACGCCAGGACCTCACTGGAGGATGAGGAAGTCTTTGAG CAAAAGCACACTAAGAAGCCTGACGGGCTGTTCAGAAATCGATTTGAAGTCAGAAACCCCGTAAAACTTC TCCAAGCAGCTGACAGCTTTAAAGACCAGACCTTCTTTCTCAGCCAGGTTTCCCAGGATGCCCTGAGAAG GACCATCTTTCCCCTGGGGGAACTAACAAAAGATTTTGTAAAGAAAATAGCCGCGGAGAATAGCCTCCAT CACGTGCTTCAGAAGAGAGAGAGCATGGGCATCTGCTTCATTGGTAAGAGAAACTTGGAGCATTTCCTTC TTCAGTATTTGCAGCCTCGGCCTGGAAAGTTTGTTTCTATCGAGGATAATACAGTTCTGGGAACACACAA AGGTTGGTTCCTGTACACTTTGGGCCAGAGAGCTAAGATCAGCGGCTTGAGAGAGCCCTGGTACGTGGTG GAGAAGGATGGCACCAAGGGTGACGTGCTTGTGGCTCCCCGGGTGGACCACCCAGCTCTGTACAGGGACC TGCTCAGGACCAACCGAGTACACTGGATTGCGGAGGAGCCACCTGCTGCCTTGGTCAGAGACAAGATGAT GGAGTGCCATTTCCGATTCCGCCACCAGATGGCACTAGTGCCCTGCGTGCTGACTCTCAATCAGGATGGC ACTGTGTGGGTAACGGCTGTGAAGGCTGTGAGGGGCCTTGCCCTGGGACAGTTTGCAGTGTTCTACAAGG GTGAGGAGTGCCTCGGCAGTGGGAAAATCCTGCGGCTAGGGCCGTCTGCCTACACACTCCAGAAGGGTAA GAACAGAACTCGAGTGGCTCCCGAGGCCTCCAGTGACAGCCCAGGCCTGCACCCCACATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206594 protein sequence
Red=Cloning site Green=Tags(s) MSALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEQGVCAADKDCEDAYKVCQILDIPFHQ VSYVKEYWNDVFSDFLNEYEKGRTPNPDINCNKHIKFSCFYHYAVDNLGADAVATGHYARTSLEDEEVFE QKHTKKPDGLFRNRFEVRNPVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGELTKDFVKKIAAENSLH HVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKFVSIEDNTVLGTHKGWFLYTLGQRAKISGLREPWYVV EKDGTKGDVLVAPRVDHPALYRDLLRTNRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDG TVWVTAVKAVRGLALGQFAVFYKGEECLGSGKILRLGPSAYTLQKGKNRTRVAPEASSDSPGLHPTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_028063 |
ORF Size | 1254 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028063.2, NP_082339.1 |
RefSeq Size | 1520 bp |
RefSeq ORF | 1254 bp |
Locus ID | 72026 |
UniProt ID | Q9DAT5 |
MW | 47.2 kDa |
Gene Summary | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211275 | Trmu (untagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
MG206594 | Trmu (tGFP-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu) |
CNY 3,710.00 |
|
MR206594L3 | Lenti ORF clone of Trmu (Myc-DDK-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
MR206594L4 | Lenti ORF clone of Trmu (mGFP-tagged) - Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |