United States
Germany
Japan
United Kingdom
China
Egln2 (NM_053208) Mouse Tagged ORF Clone
CAT＃: MR206645
- TrueORF®
Egln2 (Myc-DDK-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2)
ORF Plasmid: tGFP
"NM_053208" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610011A13Rik; C85656; Hif-p4h-1; Ier4; Phd1; SM-20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206645 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCCCGTGCCAGCCGCAGGCCCTGAATCAAGCTCTCCCTCAGTTGCCAGGGTCTGTGTCAGAGT CCTTGGAGTCTAGCCGAGCCAGAATGGGGGTGGAGAGTTACCTGCCCTGCCCTCTGCTCCCGGCCTATCA CCGTCCAGGAGCATCTGGGGAGGCCTCGGCTGGCAATGGGACCCCCAGAACCACAGCCACTGCTACTACG ACCACTGCCAGTCCCCTGCGGGAGGGCTTTGGTGGGCAGGATGGTGGTGAGCTTTGGCCACTGCAGAGTG AAGGTGCTGCTGCGTTGGTCACCAAGGAGTGCCAGCGACTGGCGGCCCAGGGTGCCCGGCCTGAGGCCCC CAAACGGAAGTGGGCCAAGGATGGTGGGGATGCCCCTTCACCCAGCAAGCGACCGTGGGCCAGGCAAGAG AACCAGGAGGCCAAGGGGGAAAGTGGTATGGGCTGTGACAGCGGTGCCAGCAACAGCAGCAGCAGCAGCA GCAACACTACCAGTAGCAGTGGCGAGGCAAGTGCTAGGCTGAGGGAGGAAGTCCAGCCCTCTGCACCTGA GCGCCTGGCCCTGGACTATATTGTGCCTTGCATGCGGTACTATGGTATCTGTGTCAAGGACAACTTCTTG GGGGCAGTACTGGGTGGCCGTGTGCTGGCTGAGGTGGAAGCCCTGAAGTGGGGCGGGCGTCTTCGTGATG GGCAACTAGTGAGCCAGCGGGCGATCCCACCGCGCAGCATTCGTGGGGACCAGATTGCCTGGGTAGAAGG TCACGAGCCAGGCTGCCGGAGCATTGGTGCCCTCATGGCTCACGTGGACGCAGTAATCCGCCACTGTGCA GGGCGGCTGGGCAACTACGTCATCAATGGGCGCACCAAGGCCATGGTGGCGTGTTATCCAGGCAATGGGC TCGGGTACGTGAGGCATGTTGACAATCCCCACGGCGATGGGCGCTGCATCACCTGTATCTATTACCTGAA TCAGAACTGGGATGTTAAGGTGCATGGCGGCCTGCTGCAGATCTTCCCCGAGGGTCGGCCAGTGGTAGCC AACATCGAGCCACTCTTTGACCGGTTGCTCATTTTCTGGTCTGACCGACGGAACCCACATGAGGTGAAGC CAGCCTATGCCACCAGGTACGCCATCACTGTCTGGTATTTTGATGCCAAGGAACGGGCAGCAGCCAGAGA CAAGTATCAGCTAGCATCGGGACAGAAAGGTGTTCAAGTACCAGTATCACAGCCAACTACACCTACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206645 protein sequence
Red=Cloning site Green=Tags(s) MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATT TTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQE NQEAKGESGMGCDSGASNSSSSSSNTTSSSGEASARLREEVQPSAPERLALDYIVPCMRYYGICVKDNFL GAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCA GRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVA NIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053208 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053208.4 |
| RefSeq Size | 2124 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 112406 |
| UniProt ID | Q91YE2 |
| MW | 45.1 kDa |
| Gene Summary | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201719 | Egln2 (untagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2), (10ug) |
CNY 3,656.00 |
|
| MG206645 | Egln2 (tGFP-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2) |
CNY 3,140.00 |
|
| MR206645L3 | Lenti ORF clone of Egln2 (Myc-DDK-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2) |
CNY 4,750.00 |
|
| MR206645L4 | Lenti ORF clone of Egln2 (mGFP-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2) |
CNY 4,750.00 |
