Foxj1 (NM_008240) Mouse Tagged ORF Clone
CAT#: MR206700
- TrueORF®
Foxj1 (Myc-DDK-tagged) - Mouse forkhead box J1 (Foxj1)
ORF Plasmid: tGFP
"NM_008240" in other vectors (4)
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CNY 3,656.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FKHL-13; HFH-4; Hfh4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206700 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGAGCTGGCTGCGCCTCTGCGGAGCGGGTCCCGGAGAGGAAGCCGGGCCGGAGGGCGGCATGG AGGAGCCGGACGCCCTGGATGACAGCCTGACCAGCCTGCAATGGCTGCAGGAATTCTCCATTCTCAACGC CAAGGCTCCCACTCTTCCCCCAGGAGGCACAGACCCCCACGGCTACCACCAAGTGCCGGGCTTGGTGGCG CCCGGGTCACCGCTGGCGGCAGACCCTGCCTGCCTTGGGCAGCCGCACACACCCGGCAAGCCCACATCGT CGTGCACATCTCGAAGCGCGCCCCCGGGGCTGCAGGCCCCGCCCCCTGACGACGTGGACTATGCCACCAA CCCACACGTGAAGCCACCCTACTCCTATGCCACTCTCATCTGCATGGCCATGCAAGCCAGCAAGGCCACC AAGATCACTCTGTCGGCCATCTACAAGTGGATCACGGACAACTTCTGCTACTTCCGCCATGCAGACCCCA CCTGGCAGAATTCCATCCGCCACAACCTGTCCTTGAACAAGTGCTTCATCAAAGTGCCTCGGGAGAAGGA TGAGCCCGGCAAGGGGGGTTTCTGGCGCATCGACCCCCAGTACGCAGAGCGCCTGCTCAGTGGGGCCTTC AAGAAGCGGAGGCTGCCCCCAGTCCACATCCACCCTGCCTTTGCCCGCCAGGCCTCCCAGGAACCTAGCG CTGCCCCCTGGGGTGGGCCTCTGACCGTGAACAGGGAGGCCCAGCAGCTGCTTCAGGAGTTTGAGGAGGC CACAGGGGAGGGGGGCTGGGGCACAGGAGAGGGCAGGCTGGGGCATAAGCGAAAGCAGCCGCTGCCCAAG AGGGTGGCCAAGGTCCTGCGGCCTCCCAGCACCTTACTGCTGACCCAGGAGGAGCAGGGTGAGCTGGAAC CACTCAAAGGTAACTTTGACTGGGAGGCCATCTTTGAGGCTGGCGCACTGGGGGAGGAGCTGAGCTCACT GGAGGGTCTGGAGCTAAGCCCCCCGCTAAGCCCCAGCTCACATGGAGATGTGGATCTTACAGTCCATGGC CGGCACATCAACTGCCCTGCTACCTGGGGACCTCCAGCGGAGCAGGCTGCTGACAGCCTGGACTTTGATG AGACCTTCTTGGGCACATCCTTCCTACAGCATCCCTGGGATGAGAGTGGTAGTGGCTGCCTGCCCCCAGA ACCCATCTTTGAAGCAGGGGATGCCACCCTGGCCGCTGACCTGCAGGACTGGGCCAGTGTGGGTGCCTTC TTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206700 protein sequence
Red=Cloning site Green=Tags(s) MAESWLRLCGAGPGEEAGPEGGMEEPDALDDSLTSLQWLQEFSILNAKAPTLPPGGTDPHGYHQVPGLVA PGSPLAADPACLGQPHTPGKPTSSCTSRSAPPGLQAPPPDDVDYATNPHVKPPYSYATLICMAMQASKAT KITLSAIYKWITDNFCYFRHADPTWQNSIRHNLSLNKCFIKVPREKDEPGKGGFWRIDPQYAERLLSGAF KKRRLPPVHIHPAFARQASQEPSAAPWGGPLTVNREAQQLLQEFEEATGEGGWGTGEGRLGHKRKQPLPK RVAKVLRPPSTLLLTQEEQGELEPLKGNFDWEAIFEAGALGEELSSLEGLELSPPLSPSSHGDVDLTVHG RHINCPATWGPPAEQAADSLDFDETFLGTSFLQHPWDESGSGCLPPEPIFEAGDATLAADLQDWASVGAF L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008240 |
ORF Size | 1266 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008240.2, NP_032266.2 |
RefSeq Size | 2599 bp |
RefSeq ORF | 1266 bp |
Locus ID | 15223 |
UniProt ID | Q61660 |
MW | 45.5 kDa |
Gene Summary | Transcription factor specifically required for the formation of motile cilia (PubMed:9096351, PubMed:9739041, PubMed:10873152, PubMed:14996907, PubMed:22357932, PubMed:27965440). Acts by activating transcription of genes that mediate assembly of motile cilia, such as CFAP157 (PubMed:27965440). Binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T) (PubMed:9096351). Activates the transcription of a variety of ciliary proteins in the developing brain and lung (PubMed:28666954, PubMed:27914912).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Co-occupancy identifies transcription factor co-operation for axon growth
,Venkatesh, I;Mehra, V;Wang, Z;Simpson, MT;Eastwood, E;Chakraborty, A;Beine, Z;Gross, D;Cabahug, M;Olson, G;Blackmore, MG;,
Nature communications
,PubMed ID 33953205
[FOXJ1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208022 | Foxj1 (untagged) - Mouse forkhead box J1 (Foxj1), (10ug) |
CNY 3,656.00 |
|
MG206700 | Foxj1 (tGFP-tagged) - Mouse forkhead box J1 (Foxj1) |
CNY 3,710.00 |
|
MR206700L3 | Lenti ORF clone of Foxj1 (Myc-DDK-tagged) - Mouse forkhead box J1 (Foxj1) |
CNY 5,230.00 |
|
MR206700L4 | Lenti ORF clone of Foxj1 (mGFP-tagged) - Mouse forkhead box J1 (Foxj1) |
CNY 6,056.00 |