United States
Germany
Japan
United Kingdom
China
Sf3b4 (NM_153053) Mouse Tagged ORF Clone
CAT＃: MR206765
- TrueORF®
Sf3b4 (Myc-DDK-tagged) - Mouse splicing factor 3b, subunit 4 (Sf3b4)
ORF Plasmid: tGFP
"NM_153053" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 49kD; Sa; Sap49; SF3b49; SF3b50 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206765 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGGACCGATCTCCGAACGGAATCAGGATGCCACGGTGTACGTGGGCGGTCTGGACGAGAAAG TGAGTGAGCCGCTGCTGTGGGAGCTCTTTCTCCAGGCAGGGCCAGTGGTCAACACCCACATGCCCAAGGA CAGAGTCACTGGCCAGCACCAGGGCTATGGCTTTGTTGAATTCCTGAGCGAGGAAGATGCCGACTATGCC ATTAAGATTATGAACATGATCAAACTCTATGGAAAGCCAATACGGGTGAACAAGGCCTCAGCTCACAACA AAAACCTGGATGTGGGAGCCAACATTTTCATTGGAAATCTGGACCCAGAAATTGATGAAAAGCTGCTTTA CGATACTTTCAGCGCCTTTGGAGTCATCCTACAGACCCCCAAGATCATGCGGGACCCTGACACAGGCAAC TCCAAGGGTTACGCCTTCATTAATTTTGCCTCCTTCGATGCTTCGGATGCAGCAATTGAGGCCATGAACG GGCAGTACCTGTGTAACCGTCCTATCACTGTGTCTTATGCCTTCAAGAAGGACTCTAAGGGTGAACGCCA TGGCTCAGCAGCTGAAAGACTCCTGGCAGCCCAGAACCCGCTGTCCCAGGCTGACCGCCCTCACCAGCTG TTTGCCGATGCACCCCCTCCGCCCTCTGCCCCCAATCCCGTGGTTTCATCCCTGGGTTCTGGGCTTCCTC CACCAGGCATGCCGCCTCCTGGCTCTTTTCCACCTCCAGTGCCACCTCCTGGGGCCCTCCCTCCTGGGAT TCCCCCAGCCATGCCCCCACCACCTATGCCACCTGGGGCTGGAGGACATGGCCCCCCAGCAGCAGGGACT CCAGGGGCTGGACATCCCGGTCACGGACACTCACATCCTCACCCGTTCCCACCAGGTGGGATGCCCCATC CAGGGATGTCCCAGATGCAGCTGGCCCACCATGGCCCCCATGGCCTAGGACACCCCCATGCTGGGCCTCC GGGCTCTGGGGGGCAGCCACCACCTCGGCCACCTCCTGGAATGCCTCATCCTGGACCTCCTCCAATGGGC ATGCCCCCCCGAGGGCCTCCTTTTGGATCTCCCATGGGTCACCCAGGTCCCATGCCTCCACACGGCATGC GTGGGCCTCCTCCATTGATGCCCCCTCATGGATACACGGGTCCTCCAAGACCCCCTCCCTATGGCTACCA GCGGGGGCCCCTCCCGCCACCCAGACCCACTCCACGGCCCCCAGTTCCACCTCGTGGTCCTCTTCGGGGC CCACTTCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206765 protein sequence
Red=Cloning site Green=Tags(s) MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYA IKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGN SKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQL FADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAGGHGPPAAGT PGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMG MPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRG PLPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153053 |
| ORF Size | 1275 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153053.4, NP_694693.1 |
| RefSeq Size | 1697 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 107701 |
| UniProt ID | Q8QZY9 |
| MW | 44.4 kDa |
| Gene Summary | This gene encodes one of the subunits of splicing factor 3B. A similar gene in human encodes a protein that cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes, and also belongs with the minor U12-dependent spliceosome. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207824 | Sf3b4 (untagged) - Mouse splicing factor 3b, subunit 4 (Sf3b4), (10ug) |
CNY 3,990.00 |
|
| MG206765 | Sf3b4 (tGFP-tagged) - Mouse splicing factor 3b, subunit 4 (Sf3b4) |
CNY 3,710.00 |
|
| MR206765L3 | Lenti ORF clone of Sf3b4 (Myc-DDK-tagged) - Mouse splicing factor 3b, subunit 4 (Sf3b4) |
CNY 5,230.00 |
|
| MR206765L4 | Lenti ORF clone of Sf3b4 (mGFP-tagged) - Mouse splicing factor 3b, subunit 4 (Sf3b4) |
CNY 5,230.00 |
