Smad3 (NM_016769) Mouse Tagged ORF Clone
CAT#: MR206770
- TrueORF®
Smad3 (Myc-DDK-tagged) - Mouse MAD homolog 3 (Drosophila) (Smad3)
ORF Plasmid: tGFP
"NM_016769" in other vectors (4)
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CNY 5,488.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AU022421; Madh3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206770 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCATCCTGCCCTTCACCCCCCCGATCGTGAAGCGCCTGCTGGGTTGGAAGAAGGGCGAGCAGA ACGGGCAGGAGGAGAAGTGGTGCGAGAAGGCGGTCAAGAGCTTGGTGAAGAAGCTCAAGAAGACGGGGCA GTTGGACGAGCTGGAGAAGGCCATCACCACGCAGAACGTGAACACCAAGTGCATTACCATCCCCAGGTCA CTGGATGGTCGGCTGCAGGTGTCCCATCGGAAGGGGCTCCCTCACGTTATCTACTGCCGCCTGTGGCGAT GGCCCGACCTGCACAGCCACCATGAATTACGGGCCATGGAGCTCTGTGAGTTTGCCTTCAACATGAAGAA GGATGAAGTGTGTGTAAATCCTTACCACTATCAGAGAGTAGAGACGCCAGTTCTACCTCCAGTGTTGGTG CCACGCCACACCGAGATCCCGGCCGAGTTCCCCCCACTGGATGACTACAGCCATTCCATTCCCGAGAACA CTAACTTCCCTGCTGGCATTGAGCCCCAGAGCAATATTCCAGAAACCCCACCTCCTGGCTACCTGAGTGA AGATGGAGAAACCAGTGACCACCAGATGAACCACAGCATGGACGCAGGTTCTCCAAACCTCTCCCCGAAT CCGATGTCCCCAGCACACAATAACTTGGACCTACAGCCAGTCACCTACTGTGAGCCGGCCTTCTGGTGCT CCATCTCCTACTACGAGCTGAACCAGCGAGTTGGGGAGACATTCCACGCCTCACAGCCATCCATGACAGT AGATGGCTTCACTGACCCCTCCAACTCGGAGCGCTTCTGCCTGGGCCTACTGTCCAATGTCAACCGGAAT GCAGCCGTGGAACTTACAAGGCGACACATTGGGAGAGGTGTGCGGCTCTACTACATCGGAGGGGAGGTCT TTGCGGAGTGCCTCAGTGACAGTGCTATTTTCGTCCAGTCTCCCAACTGCAACCAGCGCTATGGCTGGCA CCCGGCCACTGTCTGCAAGATCCCACCAGGCTGCAACCTGAAGATCTTCAACAACCAGGAATTTGCTGCC CTCCTAGCTCAGTCTGTCAACCAGGGCTTTGAGGCTGTCTACCAGCTGACGCGCATGTGCACCATCCGTA TGAGCTTCGTCAAAGGCTGGGGAGCAGAGTACAGGAGACAGACAGTGACCAGCACCCCCTGCTGGATTGA GCTACACCTGAATGGACCCTTGCAGTGGCTTGACAAGGTCCTCACCCAGATGGGTTCCCCGAGCATCCGC TGTTCCAGTGTGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206770 protein sequence
Red=Cloning site Green=Tags(s) MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRS LDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLV PRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPN PMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRN AAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAA LLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIR CSSVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016769 |
ORF Size | 1278 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016769.4 |
RefSeq Size | 5090 bp |
RefSeq ORF | 1278 bp |
Locus ID | 17127 |
UniProt ID | Q8BUN5 |
MW | 48.1 kDa |
Gene Summary | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Co-occupancy identifies transcription factor co-operation for axon growth
,Venkatesh, I;Mehra, V;Wang, Z;Simpson, MT;Eastwood, E;Chakraborty, A;Beine, Z;Gross, D;Cabahug, M;Olson, G;Blackmore, MG;,
Nature communications
,PubMed ID 33953205
[SMAD3]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207323 | Smad3 (untagged) - Mouse MAD homolog 3 (Drosophila) (Smad3), (10ug) |
CNY 5,488.00 |
|
MG206770 | Smad3 (tGFP-tagged) - Mouse MAD homolog 3 (Drosophila) (Smad3) |
CNY 7,088.00 |
|
MR206770L3 | Lenti ORF clone of Smad3 (Myc-DDK-tagged) - Mouse MAD homolog 3 (Drosophila) (Smad3) |
CNY 6,180.00 |
|
MR206770L4 | Lenti ORF clone of Smad3 (mGFP-tagged) - Mouse MAD homolog 3 (Drosophila) (Smad3) |
CNY 7,888.00 |