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Sgk3 (BC018363) Mouse Tagged ORF Clone
CAT＃: MR206839
- TrueORF®
Sgk3 (Myc-DDK-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682)
ORF Plasmid: tGFP
"BC018363" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2510015P22Rik; A330005P07Rik; Cisk; fy; fz |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206839 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTGAAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGAA GAGCAGGATTGAATGAGTTCATTCAGAACTTGGTCAGATATCCAGAGCTTTACAACCATCCAGATGTCCG AGCATTCCTTCAAATGGACAGCCCAAGACATCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTACT TCGAAGCCACATTCTACCTCACGGAACATCAACCTGGGACCAACTGGAAATCCTCATGCTAAACCAACTG ACTTCGATTTTTTAAAAGTTATTGGAAAGGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGA TGGAAAATTTTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATT ATGGCTGAACGCAATGTGCTCTTGAAAAATGTGAAGCACCCATTTTTGGTTGGATTGCACTATTCTTTCC AAACAACTGAAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTCTTCTTTCACCTCCAAAG GGAAAGGTCTTTTCCTGAACCCAGAGCGAGGTTTTATGCCGCGGAGATCGCCAGTGCCTTGGGCTACCTG CACTCCATCAAAATAGTGTACAGAGACTTGAAGCCAGAAAATATTCTTTTGGATTCAATGGGACATGTTG TCTTAACGGATTTTGGACTTTGCAAAGAAGGAATCGCTATTTCTGATACCACCACAACTTTTTGTGGTAC ACCAGAGTACCTTGCACCTGAAGTAATCAGAAAACAGCCCTATGACAACACTGTGGACTGGTGGTGCCTG GGCGCTGTTCTGTATGAGATGCTGTACGGGCTGCCTCCTTTTTACTGCCGAGATGTTGCTGAAATGTATG ACAATATTCTTCACAAGCCCTTAAACTTGAGACCAGGAGTGAGTCTCACCGCCTGGTCCATTCTGGAAGA ACTTCTAGAAAAAAACAGACAAAATCGACTTGGTGCCAAAGAAGACTTTCTTGAAATCCAGAATCATCCT TTTTTTGAGTCACTCAGCTGGACTGACCTCGTACAAAAAAAGATTCCACCTCCATTTAACCCTAATGTGG CTGGACCAGATGATATCAGAAACTTTGATGCCGTCTTCACTGAAGAAACGGTTCCCTATTCAGTGTGTGT GTCTTCTGACTATTCCATCGTGAATGCCAGTGTTCTGGAGGCAGATGATGCATTTGTTGGTTTTTCTTAC GCCCCTCCTTCGGAAGACTTATTTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206839 protein sequence
Red=Cloning site Green=Tags(s) MALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPRHQSDPSEDEDERST SKPHSTSRNINLGPTGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHI MAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEPRARFYAAEIASALGYL HSIKIVYRDLKPENILLDSMGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCL GAVLYEMLYGLPPFYCRDVAEMYDNILHKPLNLRPGVSLTAWSILEELLEKNRQNRLGAKEDFLEIQNHP FFESLSWTDLVQKKIPPPFNPNVAGPDDIRNFDAVFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSY APPSEDLFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC018363 |
| ORF Size | 1287 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018363, AAH18363 |
| RefSeq Size | 2494 bp |
| RefSeq ORF | 1289 bp |
| Locus ID | 170755 |
| MW | 91.4 kDa |
| Gene Summary | Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218544 | Sgk3 (untagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682), (10ug) |
CNY 6,940.00 |
|
| MG206839 | Sgk3 (tGFP-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682) |
CNY 2,760.00 |
|
| MR206839L3 | Lenti ORF clone of Sgk3 (Myc-DDK-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682) |
CNY 4,370.00 |
|
| MR206839L4 | Lenti ORF clone of Sgk3 (mGFP-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682) |
CNY 4,370.00 |
