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Eral1 (BC068271) Mouse Tagged ORF Clone
CAT＃: MR206974
- TrueORF®
Eral1 (Myc-DDK-tagged) - Mouse Era (G-protein)-like 1 (E. coli) (cDNA clone MGC:60473 IMAGE:30008388)
ORF Plasmid: tGFP
"BC068271" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MERA-S, MERA-W |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206974 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCCCAGGCGGTACTGCGCTGGGCTCGTTCGGGCCTTGTTAGGGGCCCGACAGGTGGGTTCGC ATGCAGGGAGGGAATGGCTTGCTCCGCCCGGCTGTCTTCTAGGTAACCAGGCGAGGTGCGTGTCCTGTGT CGTGGGCTCCACTTTCTCTGGTCCCCTCCTGGCCTCGGCCTCTAGTCGTTATGGTCAGGACTCCGCCTTG GACCGTATCCTTGGATTTTCTCAGCCTGACAGTTCGTTGGTTCCGAGCGTCCCCGCGGTGTCGGTGCACA GAGATGAGCAGAATCTACTCTTGGTGCACACTCCCGACATGCCTGAGAATCCACGGGTCCTCCGCGTGGT CCTCCTGGGAGCCCCAAATGCAGGGAAGTCAACACTTTCCAATCAGCTGCTGGGCCGTAAAGTGTTTCCT GTTTCAAAGAAGGTGCACACCACTCGATGTCAAGCTCTGGGGGTCATCACAGAGAAGGAGACCCAAGTGA TTCTGCTTGACACACCTGGCATCATCAGTCCTGTTAAACAGAAGAGGCACCATCTGGAGCGCTCTTTGCT GGAAGATCCATGGACGAGCATGGAATCTGCTGATCTTGTTGTAGTTCTTGTGGATGTGTCAGACAAGTGG ACCAGGAGTCGGCTCAACCCCCAGGTGCTGCAGTGCTTGACCAAGTTCTCTCAGGTCCCTAGCATCCTCG TCTTAAACAAGGTAGATTGCCTGAAGCAGAAGTCAGTTCTCCTGGAGCTGACAGCAGCCCTCACTGAAGG TGTTGTCAATGGCAAGAAGCTTAATATCAAGCAGGCCTTGCGCTCACGCTCCAGCACCCACTGCCCTGGC CCTGAAACTGAGGGCCCAAATGCACACTCAGTGAGGAACCCTCAGAGAATTGGCTGGCCCTACTTCCAGG AGATCTTCATGTTATCAGCACTAAACAACAAGGATGTGAACACATTGAAGCAATACCTTCTGACACAGGC CCAGCCAGGACCCTGGGAGTTCCACAGTGGTGTGCTCACTAGCCAGACACCCGAAGAGATCTGTGCCAAT AAAATCAGAGAGAAGCTCTTAGAGTACCTGCCTGAGGAGGTGCCCTACGGTGTGCAGCAGAAGACAGTGA TATGGGAGGAAGGACCAAGTGGAGAGCTAGTGATCCAACAGAACCTTCTGGTGCCCAAAGAATCTCATGT GAGGATCCTGATTGGTCAGAAGGGCCTCTCGATCTCCCAGATTGCACAGGAGGTGGGCCGGGACCTCATG GACATCTTCCACTGTGACGTTCTCATCCGCCTCTCTGTGAAACTCCTCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206974 protein sequence
Red=Cloning site Green=Tags(s) MAAPRRYCAGLVRALLGARQVGSHAGREWLAPPGCLLGNQARCVSCVVGSTFSGPLLASASSRYGQDSAL DRILGFSQPDSSLVPSVPAVSVHRDEQNLLLVHTPDMPENPRVLRVVLLGAPNAGKSTLSNQLLGRKVFP VSKKVHTTRCQALGVITEKETQVILLDTPGIISPVKQKRHHLERSLLEDPWTSMESADLVVVLVDVSDKW TRSRLNPQVLQCLTKFSQVPSILVLNKVDCLKQKSVLLELTAALTEGVVNGKKLNIKQALRSRSSTHCPG PETEGPNAHSVRNPQRIGWPYFQEIFMLSALNNKDVNTLKQYLLTQAQPGPWEFHSGVLTSQTPEEICAN KIREKLLEYLPEEVPYGVQQKTVIWEEGPSGELVIQQNLLVPKESHVRILIGQKGLSISQIAQEVGRDLM DIFHCDVLIRLSVKLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC068271 |
| ORF Size | 1311 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068271, AAH68271 |
| RefSeq Size | 2109 bp |
| RefSeq ORF | 1313 bp |
| Locus ID | 57837 |
| MW | 48.2 kDa |
| Gene Summary | Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217806 | Eral1 (untagged) - Mouse Era (G-protein)-like 1 (E. coli) (cDNA clone MGC:60473 IMAGE:30008388), (10ug) |
CNY 6,940.00 |
|
| MG206974 | Eral1 (tGFP-tagged) - Mouse Era (G-protein)-like 1 (E. coli) (cDNA clone MGC:60473 IMAGE:30008388) |
CNY 2,760.00 |
|
| MR206974L3 | Lenti ORF clone of Eral1 (Myc-DDK-tagged) - Mouse Era (G-protein)-like 1 (E. coli) (cDNA clone MGC:60473 IMAGE:30008388) |
CNY 4,370.00 |
|
| MR206974L4 | Lenti ORF clone of Eral1 (mGFP-tagged) - Mouse Era (G-protein)-like 1 (E. coli) (cDNA clone MGC:60473 IMAGE:30008388) |
CNY 4,370.00 |
