Plvap (NM_032398) Mouse Tagged ORF Clone

CAT#: MR206983

  • TrueORF®

Plvap (Myc-DDK-tagged) - Mouse plasmalemma vesicle associated protein (Plvap)

ORF Plasmid: DDK tGFP



  "NM_032398" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MECA32; Pv1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206983 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTCTCCATGGACCGCAGCCCATACGCCCGCACGGGGGACCAGCAGCGCGGCTGCTGGTACTACC
TGCGCTATTTCTTCCTCTTCGTGTCGCTCATTCAGTTCCTCATCATCCTGGGCCTGGTCCTCTTCATGAT
CTATGGCAATGTGCACGCCACCACTGAGTCCAGCCTGCGCGCCACGGAGATCCGCGCCGACAGCCTGTAC
AGCCAGGTGGTTGGACTATCGGCCTCACAGGCTAACCTGAGCAAACAGCTGAACATCAGCTTGCTTGTCA
AGGAAACAGTCATGCAGCAACTGTTGACTACGCGACGTGAGATGGAGCGCATCAACGCCAGCTTCCGCCA
GTGCCAAGGCGACCTGATCACCTACATAAACTATAATCGCTTCATCGCCGCTATCATCCTGAGCGAGAAG
CAGTGCCAGGAACAGCTGAAGGAGGTCAACAAGACCTGCGAAGCTTTACTCTTCAAGCTGGGAGAAAAAG
TTAAGACACTGGAGATGGAGGTGGCCAAGGAGAAGGCAGTGTGCTCCAAGGACAAGGAGAGCCTGCTGGC
AGGAAAGCGGCAGACGGAAGAGCAGCTGGAGGCCTGTGGCAAAGCACGCGAAAGGCAACAGCAGGAGCAG
CAGGTGACCGAGGAGAACCTGCGCAAGGTGCAGTCCCTGTGCATCCCACTGGACCAGGAGAAGTTCCAGG
CGGACGTGCTGAGTGCGTGGCGGGACTCGCTGATCTACCGTACCTTGGAAACCCTGCCCTACCACTACCA
GCTGATGCCGGAGTACGCGTCCCTCCGTCGGACCTGCGAGAGCCTGCCCGGGATCATGACCACCAAGATA
GAAGAGCTGGCCCGCGGGCTGCGCGCCGGCATTGAGCGCGTGACCCGTGAGAATGCAGAACTGCGGCGCC
AGAAGTTGGAGCTAGAGCGCGCGGCACAGGCGGCGCAGGAGGCGCGGGCGCGCGCCGGGACCGAGGCGCA
GGCGCGAGAAACCCAGCTACGGGCGGAGTGCGCGCGCCAGACGCAGCTGGCTCTGGAGGAGAAGGCGGCG
CTGCGCGCGCAGCGGGACAACCTAGAGCGCGAGCTGGAGGCGCGTAAGCGGGAGCTGGAGCAACTGCGCA
CCGAGGTGGATGTGCGCATTAGCGCGCTGGACACCTGCGTCAAGGCCAAGTCGCTGCCAGCCGTCCCGCC
GAGAGTCTCAGGCCCACCCCCGAACCCTCCACCCATTGATCCAGCTAGCCTGGAGGAATTCAAGAAAAGG
ATCCTGGAGTCTCAGCGGCTCCCTGTAGTCAACCCTGCTGCCCAACCCAGCGGT


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR206983 protein sequence
Red=Cloning site Green=Tags(s)

MGLSMDRSPYARTGDQQRGCWYYLRYFFLFVSLIQFLIILGLVLFMIYGNVHATTESSLRATEIRADSLY
SQVVGLSASQANLSKQLNISLLVKETVMQQLLTTRREMERINASFRQCQGDLITYINYNRFIAAIILSEK
QCQEQLKEVNKTCEALLFKLGEKVKTLEMEVAKEKAVCSKDKESLLAGKRQTEEQLEACGKARERQQQEQ
QVTEENLRKVQSLCIPLDQEKFQADVLSAWRDSLIYRTLETLPYHYQLMPEYASLRRTCESLPGIMTTKI
EELARGLRAGIERVTRENAELRRQKLELERAAQAAQEARARAGTEAQARETQLRAECARQTQLALEEKAA
LRAQRDNLERELEARKRELEQLRTEVDVRISALDTCVKAKSLPAVPPRVSGPPPNPPPIDPASLEEFKKR
ILESQRLPVVNPAAQPSG

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032398
ORF Size 1317 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032398.1, NM_032398.2, NP_115774.1, NP_115774.2
RefSeq Size 2033 bp
RefSeq ORF 1317 bp
Locus ID 84094
UniProt ID Q91VC4
MW 49.9 kDa
Gene Summary Endothelial cell-specific membrane protein involved in the formation of the diaphragms that bridge endothelial fenestrae. It is also required for the formation of stomata of caveolae and transendothelial channels. Functions in microvascular permeability, endothelial fenestrae contributing to the passage of water and solutes and regulating transcellular versus paracellular flow in different organs. Plays a specific role in embryonic development.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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