Cops2 (BC023096) Mouse Tagged ORF Clone

CAT#: MR207016

  • TrueORF®

Cops2 (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 2 (Arabidopsis thaliana) (cDNA clone MGC:18349

ORF Plasmid: DDK tGFP



  "BC023096" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Sgn2, Trip15, alien-like, Csn2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207016 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGATGATTTCATGTGCGATGATGAGGAGGACTACGACCTGGAGTACTCTGAAGACAGTAACTCTG
AGCCCAATGTGGATTTGGAAAATCAGTACTATAATTCCAAAGCACTGAAAGAAGATGACCCAAAAGCAGC
ATTAAGCAGCTTCCAAAAGGTTTTGGAGCTTGAAGGTGAGAAGGGAGAATGGGGATTCAAAGCGCTAAAA
CAGATGATCAAGATTAACTTCAAGTTGACAAACTTTCCAGAAATGATGAACAGATATAAGCAACTATTGA
CCTATATTCGGAGTGCAGTCACCAGGAACTATTCTGAAAAGTCCATTAATTCTATCCTTGATTATATCTC
CACTTCTAAACAGATGGATTTACTGCAGGAATTTTATGAAACAACACTGGAAGCTTTGAAAGATGCTAAG
AATGATAGACTGTGGTTTAAGACAAACACAAAGCTTGGAAAATTATATTTAGAACGAGAAGAATATGGAA
AGCTTCAAAAAATTTTACGACAGTTACATCAGTCTTGTCAGACTGATGATGGAGAAGATGACCTGAAAAA
AGGTACCCAGTTATTAGAAATCTATGCTTTGGAAATTCAAATGTACACTGCACAGAAGAACAACAAAAAG
CTTAAAGCACTCTATGAGCAATCACTTCACATCAAGTCTGCCATCCCTCACCCACTAATCATGGGTGTCA
TCAGAGAATGCGGTGGTAAAATGCACTTGAGAGAAGGTGAATTTGAAAAGGCACACACTGATTTTTTTGA
AGCCTTCAAGAATTATGATGAATCAGGAAGCCCAAGACGAACCACTTGTTTAAAATATTTGGTTTTAGCA
AATATGCTAATGAAATCAGGAATAAATCCGTTTGACTCACAAGAGGCCAAGCCGTATAAAAATGATCCAG
AAATTCTAGCAATGACAAATTTAGTAAGTGCCTATCAGAATAATGACATCACTGAATTTGAAAAGATTCT
GAAAACAAATCACAGCAACATCATGGATGATCCTTTCATAAAAGAGCACATTGAAGAGCTTTTACGAAAC
ATCAGAACACAAGTCCTCATAAAGTTAATTAAGCCTTACACAAGAATACATATTCCTTTTATTTCTAAGG
AGCTAAACATAGACGTAGCTGATGTGGAGAGCTTGCTGGTGCAGTGCATACTGGATAACACTATTCATGG
CCGAATTGATCAAGTCAACCAGCTCCTTGAACTGGATCATCAGAAGAGGGGTGGTGCCCGATACACTGCG
CTAGATAAATGGACCAACCAACTAAATTCTCTGAACCAGGCTGTGGTCAGTAAACTGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207016 protein sequence
Red=Cloning site Green=Tags(s)

MEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAALSSFQKVLELEGEKGEWGFKALK
QMIKINFKLTNFPEMMNRYKQLLTYIRSAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAK
NDRLWFKTNTKLGKLYLEREEYGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTAQKNNKK
LKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLA
NMLMKSGINPFDSQEAKPYKNDPEILAMTNLVSAYQNNDITEFEKILKTNHSNIMDDPFIKEHIEELLRN
IRTQVLIKLIKPYTRIHIPFISKELNIDVADVESLLVQCILDNTIHGRIDQVNQLLELDHQKRGGARYTA
LDKWTNQLNSLNQAVVSKLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC023096
ORF Size 1320 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC023096, AAH23096
RefSeq Size 1951 bp
RefSeq ORF 1322 bp
Locus ID 12848
MW 51.2 kDa
Gene Summary Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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