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Nfix (NM_001081982) Mouse Tagged ORF Clone
CAT＃: MR207035
- TrueORF®
Nfix (Myc-DDK-tagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1
"NM_001081982" in other vectors (3)
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CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CTF; NF-I/X; NF1-X; NFI-X |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207035 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTCCCCGTACTGCCTCACCCAGGATGAGTTCCACCCGTTTATCGAGGCGCTGCTGCCTCACGTCC GAGCCTTCTCCTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCACGAGAAGCG GATGTCAAAGGACGAGGAGCGCGCAGTGAAGGACGAGCTGCTGGGCGAGAAGCCTGAGATCAAGCAGAAG TGGGCATCCCGGCTGTTGGCCAAGCTGCGCAAAGACATCCGGCCCGAGTTCCGCGAGGACTTTGTGCTAA CCATCACGGGCAAGAAGCCCCCCTGCTGCGTGCTTTCCAACCCCGACCAGAAGGGCAAGATCCGGCGGAT TGACTGCCTGCGCCAGGCTGACAAGGTGTGGCGGCTGGACCTGGTCATGGTGATTTTGTTTAAAGGGATC CCTTTGGAAAGTACTGATGGGGAGCGGCTCTACAAGTCGCCCCAGTGCTCGAACCCCGGCCTGTGTGTCC AGCCACATCACATTGGAGTCACAATCAAAGAACTGGACCTTTATCTGGCTTACTTTGTCCACACTCCGGA ATCCGGACAATCAGATAGTTCAAACCAGCAAGGAGATGCGGACATCAAACCACTGCCCAACGGGCACTTA AGTTTCCAGGACTGCTTTGTGACGTCTGGGGTCTGGAATGTGACAGAGCTGGTGAGAGTATCACAGACTC CAGTTGCGACTGCATCAGGGCCCAACTTCTCACTGGCGGACCTGGAGAGCCCCAGCTACTACAACATAAA TCAAGTGACCCTGGGAAGGCGGTCCATCACCTCCCCTCCTTCCACCAGCAGCACCAAGCGCCCCAAGTCC ATCGACGACAGTGAGATGGAGAGTCCAGTAGATGATGTGTTCTATCCTGGGACAGGCCGCTCTCCGGCCG CTGGCAGCAGCCAGTCTAGCGGATGGCCCAATGACGTGGATGCAGGCCCTGCTTCTCTAAAGAAGTCAGG AAAGCTGGACTTCTGCAGCGCCCTCTCCTCTCAGGGCAGTTCCCCACGCATGGCTTTCACCCACCACCCG CTGCCTGTGCTTGCTGGAGTCAGACCAGGGAGCCCCCGGGCCACGGCATCAGCGCTGCACTTCCCTTCCA CGTCCATCATCCAGCAGTCGAGCCCGTATTTCACACACCCAACCATCCGCTACCACCACCACCATGGGCA GGACTCGCTGAAGGAGTTTGTGCAGTTTGTGTGCTCTGACGGCTCGGGTCAGGCCACCGGACAGCATTCA CAACGACAGGCGCCTCCTCTGCCAACCGGTTTGTCAGCATCGGATCCCGGGACGGCAACTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207035 protein sequence
Red=Cloning site Green=Tags(s) MYSPYCLTQDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEIKQK WASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGI PLESTDGERLYKSPQCSNPGLCVQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHL SFQDCFVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSSTKRPKS IDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHP LPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQHS QRQAPPLPTGLSASDPGTATF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081982 |
| ORF Size | 1326 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081982.3 |
| RefSeq Size | 5626 bp |
| RefSeq ORF | 1326 bp |
| Locus ID | 18032 |
| UniProt ID | P70257 |
| MW | 48.9 kDa |
| Gene Summary | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
High-mobility group AT-Hook 1 mediates the role of nuclear factor I/X in osteogenic differentiation through activating canonical Wnt signaling
,Wu, X;Wang, X;Shan, L;Zhou, J;Zhang, X;Zhu, E;Yuan, H;Wang, B;,
Stem cells (Dayton, Ohio) 2021
,PubMed ID 34028135
[NFIX]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200098 | Nfix (untagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
| MR207035L3 | Lenti ORF clone of Nfix (Myc-DDK-tagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1 |
CNY 5,230.00 |
|
| MR207035L4 | Lenti ORF clone of Nfix (mGFP-tagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1 |
CNY 5,230.00 |
