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Eed (NM_021876) Mouse Tagged ORF Clone
CAT＃: MR207038
- TrueORF®
Eed (Myc-DDK-tagged) - Mouse embryonic ectoderm development (Eed)
ORF Plasmid: tGFP
"NM_021876" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ENSMUSG00000039373; l(7)5Rn; l7Rn5; lusk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207038 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAGACATGCCCGCGGCCAAGAAGCAGAAGTTGA GCAGCGACGAGAACAGCAACCCGGACCTCTCGGGAGACGAAAATGACGATGCTGTCAGTATTGAGAGTGG CACAAACACAGAACGCCCGGACACACCCACAAATACGCCAAATGCACCAGGAAGGAAAAGCTGGGGAAAG GGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTGAACAGCCTCAAGGAAGATCATAACC AGCCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGAGACCCTCTGGTGTTTGCAACTGTGGG AAGCAACAGAGTAACCTTATACGAATGCCATTCACAGGGGGAGATACGGTTATTGCAGTCCTATGTCGAT GCTGATGCAGATGAAAACTTTTACACTTGTGCATGGACCTATGATAGCAACACCAGCCACCCTCTATTAG CAGTTGCTGGATCTAGAGGCATTATAAGAATAATTAATCCTATAACAATGCAGTGTATAAAGCACTATGT TGGCCATGGAAATGCTATCAATGAGCTGAAATTCCACCCACGAGACCCAAACCTTCTCCTGTCAGTAAGT AAAGATCATGCTTTACGGTTATGGAATATCCAAACAGACACTCTTGTGGCAATATTCGGAGGTGTGGAAG GGCACAGAGATGAAGTTCTGAGTGCTGATTATGATCTTTTGGGTGAAAAAATAATGTCCTGTGGTATGGA TCACTCTCTTAAACTGTGGAGAATCAACTCAAAGAGGATGATGAATGCAATTAAGGAGTCTTATGATTAT AACCCAAACAAAACTAACAGGCCATTTATTTCCCAGAAAATCCACTTTCCTGACTTTTCTACCAGAGACA TACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGATTTGATACTTTCCAAGTCTTGTGAAAATGC CATTGTATGCTGGAAACCTGGCAAAATGGAGGATGATATAGATAAAATTAAACCTAGTGAGTCTAATGTG ACTATTCTTGGGCGATTTGATTACAGCCAGTGTGACATTTGGTACATGAGGTTTTCTATGGATTTCTGGC AAAAGATGCTTGCATTGGGCAATCAGGTTGGCAAACTGTATGTTTGGGATTTAGAAGTAGAAGATCCTCA TAAAGCCAAATGCACAACACTGACCCATCATAAATGTGGCGCGGCTATTCGACAAACCAGTTTCAGTAGG GATAGCAGCATCCTCATAGCTGTCTGCGATGATGCCAGCATTTGGCGATGGGATCGACTTCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207038 protein sequence
Red=Cloning site Green=Tags(s) MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGK GKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVD ADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVS KDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDY NPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNV TILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSR DSSILIAVCDDASIWRWDRLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021876 |
| ORF Size | 1326 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021876.1, NM_021876.2, NM_021876.3, NP_068676.1 |
| RefSeq Size | 2060 bp |
| RefSeq ORF | 1326 bp |
| Locus ID | 13626 |
| UniProt ID | Q921E6 |
| MW | 50.2 kDa |
| Gene Summary | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203771 | Eed (untagged) - Mouse embryonic ectoderm development (Eed), (10ug) |
CNY 3,656.00 |
|
| MG207038 | Eed (tGFP-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 7,088.00 |
|
| MR207038L3 | Lenti ORF clone of Eed (Myc-DDK-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 5,800.00 |
|
| MR207038L4 | Lenti ORF clone of Eed (mGFP-tagged) - Mouse embryonic ectoderm development (Eed) |
CNY 5,800.00 |
