Csnk1g2 (NM_134002) Mouse Tagged ORF Clone

CAT#: MR207046

  • TrueORF®

Csnk1g2 (Myc-DDK-tagged) - Mouse casein kinase 1, gamma 2 (Csnk1g2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_134002" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 3,330.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2810429I12Rik; AI463719
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207046 representing NM_134002
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTTTGACAAGAAAGGAGGGAAGGGGGAGTTGGAGGAGGGCAGGAGAATGTCCAAAACCGGCACGA
GTCGGAGCAACCACGGCGTCCGCAGTTCGGGGACCAGCTCAGGGGTCCTGATGGTGGGCCCAAACTTCCG
AGTCGGCAAGAAGATAGGCTGTGGGAACTTCGGGGAGCTTCGCCTAGGAAAGAATCTGTATACAAATGAG
TACGTGGCTATCAAGCTGGAGCCCATCAAGTCCCGGGCCCCACAGCTGCACCTGGAGTACCGCTTTTACA
AGCAGCTCAGCACGACAGGTGAGGCGGACTCGGGTACGGGGCCAGCACTCCTAGGCCAGCAGTGGCTCCG
GACACCCAGCATGGATGTTTCCTTCGCAGAGGGCGTCCCTCAGGTCTACTACTTCGGCCCTTGTGGGAAG
TACAACGCCATGGTGCTGGAGCTGCTGGGGCCCAGCCTGGAGGACCTTTTCGACCTGTGCGACCGCACCT
TCACGCTGAAGACGGTGCTGATGATCGCCATCCAGCTGATCACGCGCATGGAGTACGTGCACACCAAGAG
CCTCATCTACCGTGACGTGAAGCCCGAGAACTTCCTGGTTGGGCGGCCGGGCAGCAAGCGGCAGCACTCC
ATCCACATCATCGACTTTGGGCTGGCCAAGGAGTACATCGACCCTGAGACTAAGAAGCACATCCCATATC
GCGAGCACAAGAGCCTGACAGGCACTGCGCGCTACATGAGCATCAACACGCACTTGGGCAAAGAGCAGAG
CCGCCGGGATGACCTGGAGGCGCTGGGACACATGTTCATGTACTTCCTGCGCGGCAGTCTGCCCTGGCAG
GGGCTCAAGGCAGACACGCTGAAGGAGCGCTACCAGAAGATTGGAGACACCAAGCGTGCCACACCAATCG
AGGTGCTGTGTGAGAGCTTCCCGGAGGAGATGGCCACCTATTTGCGCTATGTGCGGCGCCTAGACTTTTT
TGAGAAGCCAGACTACGACTACCTGAGGAAGCTCTTCACTGACCTCTTTGACCGCAGCGGCTACGTGTTT
GACTACGAGTATGACTGGGCCGGCAAGCCCCTGCCGACACCCATCGGCACCGTCCACCCTGACGTGCCCT
CCCAGCCACCACATCGCGACAAAGCTCAGCTCCACACCAAGAACCAGGCGCTCAACTCCACTAATGGAGA
GCTGAACACAGACGACCCCACCGCGGGCCACTCCAACGCCCCCATCGCGGCCCCCGCAGAAGTAGAGGTG
GCAGATGAAACAAAGTGCTGCTGCTTCTTCAAGAGGAGAAAGAGAAAATCGCTGCAGCGACATAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207046 representing NM_134002
Red=Cloning site Green=Tags(s)

MDFDKKGGKGELEEGRRMSKTGTSRSNHGVRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNE
YVAIKLEPIKSRAPQLHLEYRFYKQLSTTGEADSGTGPALLGQQWLRTPSMDVSFAEGVPQVYYFGPCGK
YNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGSKRQHS
IHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQ
GLKADTLKERYQKIGDTKRATPIEVLCESFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGYVF
DYEYDWAGKPLPTPIGTVHPDVPSQPPHRDKAQLHTKNQALNSTNGELNTDDPTAGHSNAPIAAPAEVEV
ADETKCCCFFKRRKRKSLQRHK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134002
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_134002.2, NP_598763.1
RefSeq Size 2436 bp
RefSeq ORF 1329 bp
Locus ID 103236
UniProt ID Q8BVP5
MW 50.8 kDa
Gene Summary Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...