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Sidt1 (BC025888) Mouse Tagged ORF Clone
CAT＃: MR207112
- TrueORF®
Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (cDNA clone MGC:32412 IMAGE:5039343)
ORF Plasmid: tGFP
"BC025888" in other vectors (3)
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CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW045928; B830021E24Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207112 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCTTCCGATGGTGGGCAGCCTTGCCACTCAGACACGGACAGCTCTGTGGAGGAGAGTGACTTCG ACACCATGCCTGACATCGAAAGCGACAAAAACGTCATCCGGACCAAGATGTTCTTGTACCTGTCAGATCT GTCCAGGAAGGACCGGAGGATTGTCAGCAAAAAGTATAAGATTTATTTTTGGAACATCATCACCATCGCT GTGTTTTATGCACTGCCTGTGATGCAGCTTGTTATCACCTACCAGACAGTGGTGAATGTCACTGGCAACC AGGATATCTGTTACTACAACTTCCTTTGTGCTCACCCCCTGGGTGTCCTGAGCGCCTTCAATAATATTCT CAGTAACCTGGGCCACGTACTCCTGGGCTTCCTCTTCCTGCTGATAGTTCTGCGCAGGGACCTTCTCCAC CGAAGAGCCCTGGAAGCTAAAGACATCTTTGCTATGGAGTACGGTATTCCCAAACACTTTGGTCTCTTCT ACGCTATGGGGATAGCACTGATGATGGAGGGCGTGCTCAGTGCTTGTTACCATGTCTGCCCTAATTACTC CAACTTCCAGTTCGACACTTCCTTCATGTACATGATCGCTGGCCTCTGCATGCTGAAGCTCTATCAGACC CGCCACCCGGACATCAACGCCAGTGCCTACTCTGCTTACGCCTCCTTCGCTGTGGTCATCACACTGACGG TCCTCGGAGTGGTGTTTGGAAAAAACGATGTGTGGTTCTGGATCATCTTCTCTGCCATCCACATTCTCTC TTCTCTGGCCCTCAGCACCCAAATCTACTACATGGGCCGTTTCAAGATAGATCTGGGAATCTTCCGGCGG GCTGCTATGGTGTTCTACACGGACTGTATCCAGCAATGCAGCAGACCTCTGTATATGGACAGGATGGTGT TGCTCATTGTGGGAAATCTGGTTAACTGGTCCTTTGCCTTCTTTGGATTGATCTACCGGCCCAGGGACTT TGCCTCCTACATGCTGGGCATCTTCATCTGCAACCTGCTGCTGTACTTGGCTTTCTACATCATCATGAAG CTTCGAAGCTCTGAGAAAGTCCTCCCTCTACCAGTCTTCTGCATCGCGGCCACTGCCGTGGTGTGGGCTG CGGCACTGTACTTCTTCTTCCAGAACCTCAGCAGCTGGGAGGGGACTCCTGCAGAATCCCGGGAGAAGAA CCGTGAGTGTGTCCTGCTGGATTTCTTTGATGACCATGACATCTGGCACTTCCTCTCTGCGACTGCCCTG TTTTTCTCATTCCTGGTGCTGTTGACCCTGGATGATGACCTGGATGTGGTCCGGAGAGACCAGATCCCTG TCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207112 protein sequence
Red=Cloning site Green=Tags(s) MSSSDGGQPCHSDTDSSVEESDFDTMPDIESDKNVIRTKMFLYLSDLSRKDRRIVSKKYKIYFWNIITIA VFYALPVMQLVITYQTVVNVTGNQDICYYNFLCAHPLGVLSAFNNILSNLGHVLLGFLFLLIVLRRDLLH RRALEAKDIFAMEYGIPKHFGLFYAMGIALMMEGVLSACYHVCPNYSNFQFDTSFMYMIAGLCMLKLYQT RHPDINASAYSAYASFAVVITLTVLGVVFGKNDVWFWIIFSAIHILSSLALSTQIYYMGRFKIDLGIFRR AAMVFYTDCIQQCSRPLYMDRMVLLIVGNLVNWSFAFFGLIYRPRDFASYMLGIFICNLLLYLAFYIIMK LRSSEKVLPLPVFCIAATAVVWAAALYFFFQNLSSWEGTPAESREKNRECVLLDFFDDHDIWHFLSATAL FFSFLVLLTLDDDLDVVRRDQIPVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC025888 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025888, AAH25888 |
| RefSeq Size | 2593 bp |
| RefSeq ORF | 1337 bp |
| Locus ID | 320007 |
| MW | 51.2 kDa |
| Gene Summary | In vitro binds long double-stranded RNA (dsRNA) (500 and 700 base pairs), but not dsRNA shorter than 300 bp (PubMed:26067272). Not involved in RNA autophagy, a process in which RNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Systemic RNA Interference Deficiency-1 (SID-1) extracellular domain selectively binds long double-stranded RNA and is required for RNA transport by SID-1
,Li, W;Koutmou, KS;Leahy, DJ;Li, M;,
J. Biol. Chem.
[SIDT1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207112 | Sidt1 (tGFP-tagged) - Mouse SID1 transmembrane family, member 1 (cDNA clone MGC:32412 IMAGE:5039343) |
CNY 2,850.00 |
|
| MR207112L3 | Lenti ORF clone of Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (cDNA clone MGC:32412 IMAGE:5039343) |
CNY 4,470.00 |
|
| MR207112L4 | Lenti ORF clone of Sidt1 (mGFP-tagged) - Mouse SID1 transmembrane family, member 1 (cDNA clone MGC:32412 IMAGE:5039343) |
CNY 4,470.00 |
