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Arhgap15 (BC034881) Mouse Tagged ORF Clone
CAT＃: MR207143
- TrueORF®
Arhgap15 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 15 (cDNA clone MGC:41314 IMAGE:3468639)
ORF Plasmid: tGFP
"BC034881" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5830480G12Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207143 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAATCAAAAATGCCAACAGCCACCAAGACAGGCAGAGCCAAACTAAATCCATGATCCTCACCGATG CTGGGAAGGTCACTGAACCTATTTCCCGGCACAGAAGGAATCATTCACAGCATGTGTTGAAAGATGTCAT TCCACCACTAGAACATCCAATGGTTGAAAAAGAAGGGTATCTTCAAAAAGCCAAAATTGCAGATGGAGGA AAGAAACTAAGGAAAAACTGGTCTACTTCCTGGATTGTTCTTTCTGGTCGAAAAATTGAATTTTACAAAG ACTCCAAGCAGCAAGCTCTTCCTAACATGAAAACCAGGCACAACGTGGAAAGTGTGGATTTGTGTGGTGC ACATATAGAATGGGCCAAAGAAAAATCAAGCAGAAAGAGTGTCTTTCAGATCACAACAGTGTCAGGAAAT GAGTTCCTTCTACAGTCAGATATTGACTTCCTCATATTGGATTGGTTCCAAGCTATCAAAAATGCAATTG ACAGATTGCCAAAGAATCCAAGTTGTGGGTCCCTGGAGTTGTTCAATTTGCAGAGATCCTCAAGTTCTGA ACTGCCGAGTCACTGCCACATCGATAGAAAAGAACAGAAACCAGAACACAGGAAGTCCTTCATGTTTCGA CTGCACCACAGTGCTTCTGATACAAGTGACAAGAATCGCGTGAAGAGCAGACTGAAGAAGTTCATCTCCA GAAGACCTTCTCTGAAAACTTTGCAGGAAAAGGGACTCATTAAAGATCAAATTTTTGGCTCTCATCTGCA CACAGTGTGTGAACGAGAACATTCCACAGTTCCATGGTTTGTAAAGCAATGCATTGAAGCTGTTGAAAAA AGAGGCCTAGACGTTGATGGAATTTATCGAGTTAGTGGCAATCTTGCAACAATACAGAAGTTAAGATTTA TTGTCAACCAAGAAGAGAAGCTGAATTTGGATGACAGCCAGTGGGAGGACATCCACGTCGTCACCGGAGC ACTGAAGATGTTTTTCCGGGAGCTGTCTGAACCGCTCTTCCCTTACAGTTTCTTTGAGCGGTTTGTGGAG GCGATCAAAAAACAAGACAGCAATGAAAAAATTGAAACTATGAGGTCTCTGGTAAAACGTCTCCCTCCAC CAAATCATGATACCATGAAAATCCTCTTCAGACATCTAACCAAGATAGTGGCCAAAGCCTCCCAGAATCT CATGTCCACCCAAAGCTTGGGGATTGTGTTTGGACCCACCCTTCTGCGAGCGGAAAATGAGTCAGGGAAT GTAGCGGTCCACATGGTATACCAAAACCAGATAGCAGAGTTCATGCTGACTGAGTACGATAAGATCTTCA GCTCAGAGGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207143 protein sequence
Red=Cloning site Green=Tags(s) MRIKNANSHQDRQSQTKSMILTDAGKVTEPISRHRRNHSQHVLKDVIPPLEHPMVEKEGYLQKAKIADGG KKLRKNWSTSWIVLSGRKIEFYKDSKQQALPNMKTRHNVESVDLCGAHIEWAKEKSSRKSVFQITTVSGN EFLLQSDIDFLILDWFQAIKNAIDRLPKNPSCGSLELFNLQRSSSSELPSHCHIDRKEQKPEHRKSFMFR LHHSASDTSDKNRVKSRLKKFISRRPSLKTLQEKGLIKDQIFGSHLHTVCEREHSTVPWFVKQCIEAVEK RGLDVDGIYRVSGNLATIQKLRFIVNQEEKLNLDDSQWEDIHVVTGALKMFFRELSEPLFPYSFFERFVE AIKKQDSNEKIETMRSLVKRLPPPNHDTMKILFRHLTKIVAKASQNLMSTQSLGIVFGPTLLRAENESGN VAVHMVYQNQIAEFMLTEYDKIFSSEED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034881 |
| ORF Size | 1344 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034881, AAH34881 |
| RefSeq Size | 1724 bp |
| RefSeq ORF | 1346 bp |
| Locus ID | 76117 |
| MW | 51.8 kDa |
| Gene Summary | The protein encoded by this gene is a RAC GTPase-activating protein that is regulated through its PH domain and by recruitment to the membrane. The protein accelerates hydrolysis of guanosine triphosphate to guanosine diphosphate to repress Rac activity. Knock-out of Arhgap15 function demonstrates that this gene is required to regulate multiple functions in macrophages and neutrophils. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218482 | Arhgap15 (untagged) - Mouse Rho GTPase activating protein 15 (cDNA clone MGC:41314 IMAGE:3468639), (10ug) |
CNY 6,940.00 |
|
| MG207143 | Arhgap15 (tGFP-tagged) - Mouse Rho GTPase activating protein 15 (cDNA clone MGC:41314 IMAGE:3468639) |
CNY 2,850.00 |
|
| MR207143L3 | Lenti ORF clone of Arhgap15 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 15 (cDNA clone MGC:41314 IMAGE:3468639) |
CNY 4,470.00 |
|
| MR207143L4 | Lenti ORF clone of Arhgap15 (mGFP-tagged) - Mouse Rho GTPase activating protein 15 (cDNA clone MGC:41314 IMAGE:3468639) |
CNY 4,470.00 |
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