Def8 (NM_054046) Mouse Tagged ORF Clone
CAT#: MR207144
- TrueORF®
Def8 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 8 (Def8)
ORF Plasmid: tGFP
"NM_054046" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI449518; D8Ertd713e; DEF-8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207144 representing NM_054046
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATATGATGAGAAGCTGGTTCGGTTCCGGCAGGCCCACCTCAACCCCTTCAACAAGCAGCTTGGTC CGAGGCATCATGAACAGGAACCCAGTGAGAAGGTCACTTCTGAAGACACTTTGCCTGAGCTGCCCGCTGG GGAGCCTGAATTCCACTACTCGGAGCGCATGATGGATCTCGGCCTGTCTGAGGACCACTTTTCCCGCCCT GTGGGTCTCTTCCTGGCCTCTGATGTCCAGCAGCTGCGGCAGGCCATCGAAGAATGCAAACAGGTGATCC TGGAGCTGCCCGAGCAGTCAGAGAAGCAGAAGGACGCTGTGGTGCGGCTGATCCACCTCCGGCTGAAGCT CCAGGAGCTGAAGGACCCCAATGAGGAGGAACCCAACATCCGGGTTCTCCTGGAACATCGCTTCTACAAG GAGAAGAGCAAGAGCGTTAAACAGACCTGCGATAAGTGCAACACCATCATCTGGGGGCTCATCCAGACCT GGTACACCTGCACAGGGTGTTGTTACCGCTGTCACAGCAAGTGCCTGAACCTCATCTCCAAACCATGTGT CAGCTCCAAGGTCAGTCACCAGGCTGAGTATGAGCTGAACATCTGCCCTGAGACCGGGCTGGACAGCCAG GACTACCGCTGTGCGGAGTGCCGTGCTCCCATCTCTCTGAGAGGTGTGCCTAGTGAGGCCCGGCAGTGTG ACTACACGGGCCAATACTACTGCAGCCACTGCCACTGGAACGACCTGGCTGTCATCCCCGCGAGAGTGGT GCACAACTGGGACTTTGAGCCACGCAAGGTGTCCCGTTGCAGCATGCGCTACCTGGCACTGATGGTGTCT CGGCCAGTGCTCCGGCTCCGGGAGATTAACCCTTTGCTGTTTAACTACGTGGAAGAGCTGGTGGAGATCC GGAAACTGCGCCAGGACATCCTGCTCATGAAGCCATACTTCATCACCTGCAAGGAGGCCATGGAGGCGCG ACTACTGCTGCAGCTCCAAGACAGACAGCATTTTGTGGAGAACGATGAGATGTACTCTATCCAGGACCTC TTGGAAGTGCACATGGGCCGCCTCAGCTGCTCGCTCACTGAGATCCACACGCTCTTCGCCAAGCACATCA AGTTGGACTGTGAGCGGTGCCAAGCCAAGGGGTTCGTCTGTGAACTCTGCAAAGAAGGGGACGTGCTCTT CCCGTTCGACAGCCACACGTCTGTGTGCAATGACTGTTCGGCTGTCTTCCACAGGGACTGTTACTACGAC AACTCGACCACGTGCCCCAAGTGTGCCCGGCTCACATTGAGGAAGCAGTCACTATTCCAGGAACCTGGCC TAGACATGGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207144 representing NM_054046
Red=Cloning site Green=Tags(s) MEYDEKLVRFRQAHLNPFNKQLGPRHHEQEPSEKVTSEDTLPELPAGEPEFHYSERMMDLGLSEDHFSRP VGLFLASDVQQLRQAIEECKQVILELPEQSEKQKDAVVRLIHLRLKLQELKDPNEEEPNIRVLLEHRFYK EKSKSVKQTCDKCNTIIWGLIQTWYTCTGCCYRCHSKCLNLISKPCVSSKVSHQAEYELNICPETGLDSQ DYRCAECRAPISLRGVPSEARQCDYTGQYYCSHCHWNDLAVIPARVVHNWDFEPRKVSRCSMRYLALMVS RPVLRLREINPLLFNYVEELVEIRKLRQDILLMKPYFITCKEAMEARLLLQLQDRQHFVENDEMYSIQDL LEVHMGRLSCSLTEIHTLFAKHIKLDCERCQAKGFVCELCKEGDVLFPFDSHTSVCNDCSAVFHRDCYYD NSTTCPKCARLTLRKQSLFQEPGLDMDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_054046 |
ORF Size | 1344 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_054046.5, NP_473387.1 |
RefSeq Size | 3786 bp |
RefSeq ORF | 1347 bp |
Locus ID | 23854 |
UniProt ID | Q99J78 |
MW | 52.3 kDa |
Gene Summary | Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). Involved in bone resorption (PubMed:27777970).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215979 | Def8 (untagged) - Mouse differentially expressed in FDCP 8 (Def8), (10ug) |
CNY 5,800.00 |
|
MG207144 | Def8 (tGFP-tagged) - Mouse differentially expressed in FDCP 8 (Def8) |
CNY 3,710.00 |
|
MR207144L3 | Lenti ORF clone of Def8 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 8 (Def8) |
CNY 5,230.00 |
|
MR207144L4 | Lenti ORF clone of Def8 (mGFP-tagged) - Mouse differentially expressed in FDCP 8 (Def8) |
CNY 5,230.00 |