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Gramd1c (NM_153528) Mouse Tagged ORF Clone
CAT＃: MR207298
- TrueORF®
Gramd1c (Myc-DDK-tagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 1
ORF Plasmid: tGFP
"NM_153528" in other vectors (4)
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CNY 3,656.00
CNY 4,090.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921521N14Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207298 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACACTTGTTATCAGTTGAAGAAAATGTGCAGCCAAGAAGTCCAGGAAGAAGCAGCGTGGATGACG CTGGGGAAAGAGATGAGAAGTTCTCCAAGGCCGTCAGCTTTACACAGGAGTCAGTTAGCAGAGCTTCAGA AACAGAGCCATTGGACGGGAACTCACCGAAAAGAGGACTAGGAAAAGAGGATTCCCAGAGCGAGAGAAAT GTGAGAAAAAGTCCTTCGCTAGCTTCAGAAAAGAGGATAAGCAGAGCACCCTCCAAGTCACTGGACTTGA ATAAGAATGAGTACCTTTCTCTGGATAAAAGCAGCACTTCAGATTCTGTGGACGAAGAAAATATTCCCGA GAAAGATCTTCAAGGAAGACTTTATATCAACCGTGTCTTTCACATCAGTGCTGAGAGAATGTTCGAACTG CTGTTCACTAGCTCACACTTTATGCAGAGATTTGCAAATTCTAGAAATATAATAGATGTGGTATCTACCC CCTGGACAGTTGAATCTGGAGGCAATCAGCTGCGAACCATGACCTATACCATAGTCCTCAGCAACCCGCT AACTGGGAAGTATACTGCTGCCACAGAAAAGCAGACCCTGTATAAAGAGAGCCGGGAAGCACAGTTCTAC CTGGTAGACTCCGAAGTGCTGACACATGATGTGCCCTATCACGACTACTTCTACACTTTGAACAGATACT GTATCGTGAGATCTGCAAAACAGAGATGCAGGCTGAGAGTCTCCACAGACTTGAAGTACAGGAAACAACC ATGGGGCCTTATCAAGTCTTTAATTGAGAAGAATTCCTGGAGTTCACTGGAGAGCTACTTCAAAAAGCTT GAATCCGATTTGTTAATGGAAGAGTCTGTGTTGAGTCAATCCATTGAAGATGCTGGAAAACATAGCAGCC TACGCCGGAGAAGGCGGACCTTGAACCGGACAGCAGAGCCCGTTCCAAAGCTGTCCTCTCAGCGCTCTTC CACAGATTTGGGCTTCGAGGCCAAAGTAGATGTTACAGGAAAGAGAAAGACCGTGGACAGTTATGACACC GCCCTTATTGTGGTGATGAGCATATTCTTGCTGCTGCTCGTTCTGCTGAATGTGACACTATTTCTGAAGC TGTCAAAGATAGAACACGCTACCCAGTCCTTCTACCAGCTTCACCTCCAGGGAGAAAAATCTTTAAATTT AGTCTCTGACAGGTTCTCAAGAACAGAAAATATTCAAAAGAACAAAGATCAGGCCCACCGCCTAAAAGGA GTACTCCAAGATTCCATAGTGATGTTGGAACAGCTGAAGAGCTCACTCATTATGCTTCAAAAAACCTTTG ATTTACTAAATAAGAACAAGTCTGGGGTGGCTGTGGAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207298 protein sequence
Red=Cloning site Green=Tags(s) MEHLLSVEENVQPRSPGRSSVDDAGERDEKFSKAVSFTQESVSRASETEPLDGNSPKRGLGKEDSQSERN VRKSPSLASEKRISRAPSKSLDLNKNEYLSLDKSSTSDSVDEENIPEKDLQGRLYINRVFHISAERMFEL LFTSSHFMQRFANSRNIIDVVSTPWTVESGGNQLRTMTYTIVLSNPLTGKYTAATEKQTLYKESREAQFY LVDSEVLTHDVPYHDYFYTLNRYCIVRSAKQRCRLRVSTDLKYRKQPWGLIKSLIEKNSWSSLESYFKKL ESDLLMEESVLSQSIEDAGKHSSLRRRRRTLNRTAEPVPKLSSQRSSTDLGFEAKVDVTGKRKTVDSYDT ALIVVMSIFLLLLVLLNVTLFLKLSKIEHATQSFYQLHLQGEKSLNLVSDRFSRTENIQKNKDQAHRLKG VLQDSIVMLEQLKSSLIMLQKTFDLLNKNKSGVAVES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153528 |
| ORF Size | 1374 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153528.2, NP_705756.1 |
| RefSeq Size | 3506 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 207798 |
| UniProt ID | Q8CI52 |
| MW | 52.2 kDa |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Aster‐C coordinates with COP I vesicles to regulate lysosomal trafficking and activation of mTORC1
,null,
EMBO Reports
,PubMed ID 32648345
[Gramd1c]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216185 | Gramd1c (untagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 1, (10ug) |
CNY 5,990.00 |
|
| MG207298 | Gramd1c (tGFP-tagged) - Mouse GRAM domain containing 1C (Gramd1c) |
CNY 5,256.00 |
|
| MR207298L3 | Lenti ORF clone of Gramd1c (Myc-DDK-tagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 1 |
CNY 5,990.00 |
|
| MR207298L4 | Lenti ORF clone of Gramd1c (mGFP-tagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 1 |
CNY 5,990.00 |
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