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Nfs1 (NM_010911) Mouse Tagged ORF Clone
CAT＃: MR207324
- TrueORF®
Nfs1 (Myc-DDK-tagged) - Mouse nitrogen fixation gene 1 (S. cerevisiae) (Nfs1)
ORF Plasmid: tGFP
"NM_010911" in other vectors (4)
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CNY 3,656.00
CNY 4,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA987187; m-Nfs1; m-Nfsl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207324 representing NM_010911
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGCTCTGTGGCTGGGAACATGCTGCTGCGAGCCGCTTGGAGGCGGGCGTCGTTGGCGGCTACCT CCTTGGCCCTGGGAAGGTCCTCGGTGCCCACCCGGGGACTGCGCCTGCGCGTTGTAGATCATGGCCCCCA TTCGCCTGTTCACTCAGAGGCAGAGGCAGTGCTGCGACCTCTCTACATGGACGTACAGGCCACCACTCCT CTGGATCCCAGAGTGCTTGATGCCATGCTCCCATACCTTGTCAACTACTATGGGAACCCTCATTCTCGGA CTCATGCATATGGCTGGGAGAGCGAGGCAGCCATGGAACGTGCTCGCCAGCAAGTAGCATCTCTGATTGG AGCTGATCCTCGGGAGATCATTTTCACTAGTGGAGCTACTGAGTCCAACAACATAGCAATTAAGGGAGTG GCCAGGTTCTACAGGTCAAGGAAAAAACACTTGGTCACAACCCAGACAGAACACAAATGTGTGCTGGATT CCTGCCGCTCCCTGGAAGCTGAGGGCTTTCGGGTCACTTACCTCCCTGTGCAGAAGAGCGGGATCATTGA CTTAAAGGAACTAGAGGCTGCCATCCAGCCAGACACCAGCCTGGTCTCCGTTATGACTGTGAACAATGAA ATTGGAGTAAAGCAACCAATTGCAGAAATAGGGCAGATTTGTAGTTCCAGAAAGGTGTACTTCCACACTG ATGCCGCCCAAGCGGTTGGGAAAATCCCACTTGACGTCAATGACATGAAGATTGATCTCATGAGCATCAG CGGTCACAAACTCTATGGCCCTAAAGGGGTTGGTGCTATCTATATCCGCCGTAGGCCCCGGGTACGTGTG GAGGCCCTACAGAGTGGAGGCGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTGGTGG TGGGCCTGGGAGCTGCATGTGAGTTGGCACAACAAGAGATGGAATACGACCATAAGCGGATCTCAAAGTT AGCAGAGCGACTGGTACAGAACATAATGAAGAATCTTCCAGATGTGGTAATGAACGGGGACCCGAAGCAG CACTACCCTGGCTGTATCAACCTCTCCTTCGCGTATGTGGAAGGAGAGAGCCTGCTGATGGCCCTCAAGG ATGTGGCCTTGTCCTCAGGGAGTGCCTGCACTTCTGCATCATTGGAGCCCTCTTATGTCCTCAGAGCAAT TGGTACCGATGAGGATTTAGCACACTCCTCTATCAGGTTTGGCATCGGCCGCTTCACTACAGAAGAGGAG GTGGACTACACGGCGGAGAAGTGCATCCACCACGTGAAGCGCCTTCGAGAAATGAGTCCTCTCTGGGAGA TGGTCCAGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207324 representing NM_010911
Red=Cloning site Green=Tags(s) MVGSVAGNMLLRAAWRRASLAATSLALGRSSVPTRGLRLRVVDHGPHSPVHSEAEAVLRPLYMDVQATTP LDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGV ARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNE IGVKQPIAEIGQICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRV EALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQ HYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEE VDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010911 |
| ORF Size | 1377 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010911.2, NP_035041.2 |
| RefSeq Size | 2053 bp |
| RefSeq ORF | 1380 bp |
| Locus ID | 18041 |
| UniProt ID | Q9Z1J3 |
| MW | 51 kDa |
| Gene Summary | Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200702 | Nfs1 (untagged) - Mouse nitrogen fixation gene 1 (S. cerevisiae) (Nfs1), (10ug) |
CNY 3,656.00 |
|
| MG207324 | Nfs1 (tGFP-tagged) - Mouse nitrogen fixation gene 1 (S. cerevisiae) (Nfs1) |
CNY 4,470.00 |
|
| MR207324L3 | Lenti ORF clone of Nfs1 (Myc-DDK-tagged) - Mouse nitrogen fixation gene 1 (S. cerevisiae) (Nfs1) |
CNY 5,990.00 |
|
| MR207324L4 | Lenti ORF clone of Nfs1 (mGFP-tagged) - Mouse nitrogen fixation gene 1 (S. cerevisiae) (Nfs1) |
CNY 5,990.00 |
