Ctsf (NM_019861) Mouse Tagged ORF Clone

CAT#: MR207380

  • TrueORF®

Ctsf (Myc-DDK-tagged) - Mouse cathepsin F (Ctsf)

ORF Plasmid: DDK tGFP



  "NM_019861" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00

CNY 4,090.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI481912
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207380 representing NM_019861
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCCCTACTGCAGCTTCTGTGGCTGCTGACACTGCTCTCGACTGTGGCCCTGAGCCCCGTCCCCG
CCAAGCCCTGGGCAGATGACGAGCAGGCCTGGAACTTGTCGTCCCAGGAGCTGCTGGCCCCCGCCCGCTT
CGCGCTGGACATGTACAATTACGGCCGTGCTGCGGGGACAAGGGCCGTGCTAGGAGCGGTGCGCGGACGC
GTCCGCCGGGCCGGCCAGGGCTCTCTGTTCTCCCTGGAAGCCACACTAGAGGAGCCACCTTGCAATGATC
CCCTGGTGTGCCCACTGCCTGAGACGAAGAAAACAGTGCTCTGCAGTTTTGAAGTCCTGGAAGAGCTAAA
AGAACACTTGCTGCTGAGGAGGGACTGTAGCCCAGTGAATGCCAAGGTCACAGAGTTTAGAAATGCGACT
TTCAGCTCATTCCTTCCATTGTTGGACAAGGATCCCCTGCCCCAGGACTTCTCTGTGAAGATGGCTCCAC
TCTTCAAGGACTTCATGACCACCTATAACCGGACTTATGAATCAAGGGAAGAAGCCCAGTGGCGATTGAC
TGTCTTTGCAAGAAATATGATACGAGCACAGAAGATTCAGGCCCTGGACCGCGGCACAGCTCAGTATGGG
ATCACCAAGTTCAGTGACCTCACAGAGGAGGAATTCCACACCATCTACCTGAATCCCCTCTTACAGAAGG
AGTCTGGCAGGAAGATGAGTCCAGCCAAGTCCATAAATGATCTTGCCCCGCCTGAATGGGACTGGAGGAA
GAAAGGGGCTGTCACTGAAGTGAAGAACCAGGGCATGTGTGGCTCCTGCTGGGCCTTTTCTGTCACAGGC
AACGTGGAGGGCCAGTGGTTCCTGAACCGGGGGACTCTGCTCTCCCTGTCAGAGCAGGAGCTCTTGGATT
GTGACAAGGTGGACAAGGCCTGCTTGGGTGGATTGCCCTCCAACGCCTATGCAGCCATAAAGAATTTGGG
AGGGCTGGAGACAGAGGATGACTACGGCTACCAGGGCCATGTTCAGACCTGCAACTTCTCAGCACAGATG
GCAAAAGTCTACATCAATGATTCAGTGGAGCTGAGCCGGAATGAAAATAAGATAGCAGCCTGGCTGGCCC
AGAAAGGACCTATCTCAGTTGCCATTAACGCCTTCGGCATGCAGTTCTATCGCCACGGGATTGCTCACCC
ATTCCGGCCCCTCTGCAGCCCTTGGTTCATCGACCATGCTGTGTTGCTGGTGGGCTATGGCAACCGCTCT
AACATTCCTTACTGGGCCATCAAGAACAGCTGGGGCAGTGACTGGGGTGAGGAGGGTTACTACTACTTGT
ACCGTGGATCTGGAGCCTGTGGTGTGAATACCATGGCCAGCTCAGCCGTGGTGAAC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR207380 representing NM_019861
Red=Cloning site Green=Tags(s)

MAPLLQLLWLLTLLSTVALSPVPAKPWADDEQAWNLSSQELLAPARFALDMYNYGRAAGTRAVLGAVRGR
VRRAGQGSLFSLEATLEEPPCNDPLVCPLPETKKTVLCSFEVLEELKEHLLLRRDCSPVNAKVTEFRNAT
FSSFLPLLDKDPLPQDFSVKMAPLFKDFMTTYNRTYESREEAQWRLTVFARNMIRAQKIQALDRGTAQYG
ITKFSDLTEEEFHTIYLNPLLQKESGRKMSPAKSINDLAPPEWDWRKKGAVTEVKNQGMCGSCWAFSVTG
NVEGQWFLNRGTLLSLSEQELLDCDKVDKACLGGLPSNAYAAIKNLGGLETEDDYGYQGHVQTCNFSAQM
AKVYINDSVELSRNENKIAAWLAQKGPISVAINAFGMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGNRS
NIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASSAVVN

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019861
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_019861.2
RefSeq Size 1975 bp
RefSeq ORF 1389 bp
Locus ID 56464
UniProt ID Q9R013
MW 51.7 kDa
Gene Summary Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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