L2hgdh (NM_145443) Mouse Tagged ORF Clone

CAT＃: MR207410

  • TrueORF®

L2hgdh (Myc-DDK-tagged) - Mouse L-2-hydroxyglutarate dehydrogenase (L2hgdh), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_145443" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 3,656.00

CNY 4,090.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms BC016226
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207410 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCCGACCCTGCGCTACGTAGGCGGTGTCTGCGGACTGGCCCGGTACTGCGTGGCTGGGGGCTTCC
TCCGCGCAAGTGGACCGGCGTCCGGGGTGCCCGGGCTACTGTGCGGGGGTGGCCGCAGAAGCTCCAGCAC
CAGCTCTTTTGATATAGTCATCGTTGGAGGTGGAATTGTGGGCCTTGCCTCTGCCAGAACGCTCATCCTG
AAACATCCTGGACTTTCGATTGGTGTGGTGGAAAAGGAGAAAGATTTAGCTCTTCACCAGACTGGACACA
ACAGCGGTGTCATACACAGTGGTATTTACTATAAACCAGAATCTCTGAAGGCTAAATTGTGTGTAGAAGG
CGCAGCCCTCATCTATGAGTACTGCAACCTCAAGGGAATTCCCTACAGGCAATGTGGCAAGCTAATAGTA
GCTGTTGAACAAGAAGAAATTCCCAGACTTCAGGCCTTGTATGAGAGAGGCCTGCAGAATGGAGTCGAAG
GCCTGAGGCTGATCCAGCAGGAGGATATAAAAAAGAAGGAGCCGTATTGCAGGGGTCTAATGGCTATTGA
TTGTCCATACACCGGCATTGTGAACTACCAACAGGTAGCTTTGTCATTTGCCCAGGATTTCCAAGAAGCA
GGTGGCTCTATCTTGAGAGATTTTGAGGTAAAAGGTATTGAAATAGCTAAAGAAAACTCTTCAAGAAGTA
AAGATGGCATGAACTATCCAATCGCTGTCAAGAACAGCAAGGGAAAGGAGATTCGGTGTCGATATGTTGT
CACATGTGCTGGTCTTTACTCTGACCGTATTTCAGAGTTGAGTGGCTGCAATCCTGACCCTCAAATTGTC
CCATTCCGGGGAGATTACCTGGTCTTAAAGCCAGAAAAGGGCTACCTTGTAAAAGGAAATATTTATCCGG
TCCCAGACAGCCGGTTTCCTTTCCTCGGAGTTCACTTTACGCCCAGGTTGGATGGCACTATTTGGCTAGG
GCCTAATGCAGTGCTTGCCTTTAAACGGGAAGGTTACAGACCCTTTGACTTCGATGCCAGAGATGTTATG
GAAGTAATTCTCAAAAGCGGCTTCATTAACCTGGTGTTCCAGCATTTCTCTTATGGAGTTAATGAAATGT
ATAAAGCCTGTTTTCTCAGTGAAACAGTGAAGCATCTTCAAAAGTTCATTCCTGAAATCACCATCAGCGA
TGTCCTTCGAGGTCCTGCTGGAGTACGGGCCCAGGCCCTGGACAGAGATGGAAATCTGGTAGAGGATTTT
GTGTTTGACGGAGGGACTGGCGAGATTGCAGACCGCGTCCTTCATGTGAGAAATGCGCCTTCGCCTGCTG
CCACTTCCTCCCTGGCAATTTCTAGAATGATAGCAGAGGAAGCACAGCAAAGGTTTAAGTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR207410 protein sequence
Red=Cloning site Green=Tags(s)

MWPTLRYVGGVCGLARYCVAGGFLRASGPASGVPGLLCGGGRRSSSTSSFDIVIVGGGIVGLASARTLIL
KHPGLSIGVVEKEKDLALHQTGHNSGVIHSGIYYKPESLKAKLCVEGAALIYEYCNLKGIPYRQCGKLIV
AVEQEEIPRLQALYERGLQNGVEGLRLIQQEDIKKKEPYCRGLMAIDCPYTGIVNYQQVALSFAQDFQEA
GGSILRDFEVKGIEIAKENSSRSKDGMNYPIAVKNSKGKEIRCRYVVTCAGLYSDRISELSGCNPDPQIV
PFRGDYLVLKPEKGYLVKGNIYPVPDSRFPFLGVHFTPRLDGTIWLGPNAVLAFKREGYRPFDFDARDVM
EVILKSGFINLVFQHFSYGVNEMYKACFLSETVKHLQKFIPEITISDVLRGPAGVRAQALDRDGNLVEDF
VFDGGTGEIADRVLHVRNAPSPAATSSLAISRMIAEEAQQRFKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145443
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145443.1, NP_663418.1
RefSeq Size 3338 bp
RefSeq ORF 1395 bp
Locus ID 217666
UniProt ID Q91YP0
MW 50.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Signaling metabolite L-2-hydroxyglutarate activates the transcription factor HIF-1α in lipopolysaccharide-activated macrophages ,null, The Journal of Biological Chemistry ,PubMed ID 34929172 [L2hgdh]

Other Versions

SKU Description Size Price
MG207410 L2hgdh (tGFP-tagged) - Mouse L-2-hydroxyglutarate dehydrogenase (L2hgdh)
    • 10 ug

CNY 4,470.00

MR207410L3 Lenti ORF clone of L2hgdh (Myc-DDK-tagged) - Mouse L-2-hydroxyglutarate dehydrogenase (L2hgdh), nuclear gene encoding mitochondrial protein
    • 10 ug

CNY 6,056.00

MR207410L4 Lenti ORF clone of L2hgdh (mGFP-tagged) - Mouse L-2-hydroxyglutarate dehydrogenase (L2hgdh), nuclear gene encoding mitochondrial protein
    • 10 ug

CNY 5,990.00

You may also need
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