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Sox10 (BC023356) Mouse Tagged ORF Clone
CAT＃: MR207452
- TrueORF®
Sox10 (Myc-DDK-tagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:32314 IMAGE:5027211)
ORF Plasmid: tGFP
"BC023356" in other vectors (4)
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CNY 4,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dom; gt; Sox21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207452 representing BC023356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGAACAAGACCTATCAGAGGTGGAGCTGAGCCCTGTGGGCTCGGAGGAACCCCGCTGCCTGT CCCCAGGCAGCGCGCCGTCGCTGGGACCCGACGGCGGCGGCGGTGGCTCGGGCTTGCGAGCCAGCCCGGG GCCCGGTGAACTGGGCAAGGTCAAGAAGGAACAGCAGGACGGCGAGGCGGACGATGACAAGTTCCCCGTG TGCATCCGCGAGGCGGTCAGCCAGGTGCTCAGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCG TCAACGGTGCCAGCAAGAGCAAGCCGCACGTCAAGAGGCCCATGAACGCCTTCATGGTGTGGGCACAGGC GGCACGCAGAAAGCTAGCCGACCAGTACCCTCACCTCCACAATGCTGAGCTCAGCAAGACACTAGGCAAG CTCTGGAGGTTGCTGAACGAAAGTGACAAGCGCCCCTTCATTGAGGAGGCTGAGCGGCTCCGGATGCAGC ACAAAAAGGACCATCCGGACTACAAGTACCAACCTCGGCGGCGGAAGAACGGGAAGGCAGCCCAGGGGGA GGCAGAATGCCCAGGCGGGGAAGCCGAGCAAGGAGGGGCTGCTGCTATTCAGGCTCACTACAAGAGTGCC CACCTGGACCACCGGCACCCAGAAGAAGGCTCCCCCATGTCAGATGGGAACCCAGAGCACCCCTCAGGCC AGAGCCATGGCCCCCCAACCCCTCCAACCACCCCAAAGACAGAGCTGCAGTCCGGCAAGGCAGACCCCAA AAGGGATGGGCGCTCCTTGGGGGAGGGCGGGAAGCCCCACATCGACTTCGGCAACGTGGACATCGGGGAG ATCAGCCACGAGGTAATGTCCAACATGGAAACCTTTGATGTGACTGAGCTGGACCAATACCTGCCACCCA ACGGGCACCCAGGCCATGTGGGTAGCTACTCGGCAGCTGGCTATGGGCTGGGCAGTGCCCTGGCTGTGGC CAGTGGACACTCTGCCTGGATCTCCAAGCCACCAGGTGTGGCTCTGCCCACGGTCTCGCCCCCTGGTGTG GATGCCAAAGCCCAGGTGAAGACAGAGACCACAGGCCCCCAGGGACCCCCACACTACACCGACCAGCCGT CCACTTCCCAGATCGCCTACACCTCCCTCAGTCTGCCCCACTACGGCTCCGCCTTCCCCTCCATCTCACG ACCCCAGTTTGACTATTCTGACCATCAGCCCTCAGGACCCTATTATGGCCATGCAGGCCAGGCCTCTGGC CTCTATTCAGCCTTTTCTTACATGGGGCCCTCCCAGCGGCCCCTCTACACTGCCATCTCTGACCCCAGCC CCTCGGGGCCCCAATCCCACAGTCCCACACACTGGGAGCAGCCAGTATATACGACTCTATCCCGACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207452 representing BC023356
Red=Cloning site Green=Tags(s) MAEEQDLSEVELSPVGSEEPRCLSPGSAPSLGPDGGGGGSGLRASPGPGELGKVKKEQQDGEADDDKFPV CIREAVSQVLSGYDWTLVPMPVRVNGASKSKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGK LWRLLNESDKRPFIEEAERLRMQHKKDHPDYKYQPRRRKNGKAAQGEAECPGGEAEQGGAAAIQAHYKSA HLDHRHPEEGSPMSDGNPEHPSGQSHGPPTPPTTPKTELQSGKADPKRDGRSLGEGGKPHIDFGNVDIGE ISHEVMSNMETFDVTELDQYLPPNGHPGHVGSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGV DAKAQVKTETTGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHAGQASG LYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023356 |
| ORF Size | 1398 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023356.1 |
| RefSeq Size | 2600 bp |
| RefSeq ORF | 1400 bp |
| Locus ID | 20665 |
| MW | 95.3 kDa |
| Gene Summary | Transcription factor that plays a central role in developing and mature glia (PubMed:24204311, PubMed:27532821). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (PubMed:24204311, PubMed:27532821). Once induced, MYRF cooperates with SOX10 to implement the myelination program (PubMed:24204311). Transcriptional activator of MITF, acting synergistically with PAX3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218240 | Sox10 (untagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:32314 IMAGE:5027211), (10ug) |
CNY 6,940.00 |
|
| MG207452 | Sox10 (tGFP-tagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:32314 IMAGE:5027211) |
CNY 7,456.00 |
|
| MR207452L3 | Lenti ORF clone of Sox10 (Myc-DDK-tagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:32314 IMAGE:5027211) |
CNY 6,560.00 |
|
| MR207452L4 | Lenti ORF clone of Sox10 (mGFP-tagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:32314 IMAGE:5027211) |
CNY 6,560.00 |
