Mtmr7 (BC032254) Mouse Tagged ORF Clone

CAT#: MR207467

  • TrueORF®

Mtmr7 (Myc-DDK-tagged) - Mouse myotubularin related protein 7 (cDNA clone MGC:40839 IMAGE:5368816)

ORF Plasmid: DDK tGFP



  "BC032254" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207467 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCACATCCGCACGCCCAAGGTTGAAAACGTGCGCTTGGTAGACAGAGTGTCTTGTAAAAAGGCAG
CGCTGGGAACTTTGTATCTGACGGCCACTCACGTCATATTCGTGGAAAATGCACCTGACACAAGAAAAGA
AACCTGGATTCTGCACAGTCAGATCTCCACCATTGAAAAGCAGGCGACAACAGCTACGGGCTGCCCTCTG
CTTATTCGCTGCAAGAACTTCCAGATCGTACAACTTGTCATTCCACAGGAGAGAGACTGCCACGACGTGT
ACATCTCTCTGATACGCCTTGCACGGCCAGTGAAGTACGAGGAGCTATACTGCTTTTCCTTCAACCCCAA
GCTGGATAAGGAAGAACGGGAGCAGGGCTGGTTGCTGGTTGACCTTAGTGAAGAATACAAGCGAATGGGC
CTCCCTGATAATTATTGGCAGCTCAGCGATGTGAATAGAGACTACAGAGTTTGTGACTCTTACCCCACGG
AACTGTACGTCCCCAGATCGGCCACGGCACACATCATCGTGGGCAGCTCCAAGTTCCGGAGCAGACGGCG
ATTTCCTGCCCTTTCCTACTACTGTAAAGACAGCCATGCCTCCATCTGCAGGAGCAGCCAGCCCCTTTCC
GGCTTCAGCGCTCGGTGCCTGGAGGATGAGCAGATGCTCCAAGCCATCAGGAAAGCCAATCCTGGAAGTG
ACTTCATTTATGTCGTGGACACAAGGCCTAAACTTAATGCGATGGCAAATCGTGCTGCAGGGAAAGGCTA
TGAGAATGAAGACAATTATTCCAATATCAAGTTTCAGTTTATCGGCATAGAGAACATCCACGTCATGAGG
AACAGTCTTCAGAAAATGCTGGAAGTGTGTGAACTTAAATCTCCCTCTATGAGTGATTTCCTGTGGGGAC
TGGAGAACTCAGGCTGGCTGAGGCACATCAAAGCCATCATGGATGCAGGGATCTTCATTGCAAAGGCAGT
TTCAGAGGAAGGGGCAAGTGTGCTTGTTCACTGTTCTGACGGCTGGGACAGAACGGCCCAGGTGTGCTCG
GTGGCTAGCCTGCTGCTGGACCCTTACTACCGGACTCTGAAAGGATTCATGGTATTAATTGAAAAGGACT
GGATTTCCTTCGGACATAAATTTAATCATAGATATGGCAACTTAGATGGTGACCCAAAAGAAATCTCTCC
AGTAATCGACCAGTTCATTGAGTGTGTCTGGCAGCTAACGGAACAGTTCCCTTGCGCCTTTGAGTTCAAC
GAGAGGTTTCTGACTCACATTCAGCATCATGTTTATTCCTGCCAGTTTGGGAACTTCCTGTGTAATAGCC
AGAAGGAGAGGCGAGAACTCAATGAGAATGGAAAAAGCAAAAAGGGTTCACGGTTCCTTAAGGAGGACTC
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207467 protein sequence
Red=Cloning site Green=Tags(s)

MEHIRTPKVENVRLVDRVSCKKAALGTLYLTATHVIFVENAPDTRKETWILHSQISTIEKQATTATGCPL
LIRCKNFQIVQLVIPQERDCHDVYISLIRLARPVKYEELYCFSFNPKLDKEEREQGWLLVDLSEEYKRMG
LPDNYWQLSDVNRDYRVCDSYPTELYVPRSATAHIIVGSSKFRSRRRFPALSYYCKDSHASICRSSQPLS
GFSARCLEDEQMLQAIRKANPGSDFIYVVDTRPKLNAMANRAAGKGYENEDNYSNIKFQFIGIENIHVMR
NSLQKMLEVCELKSPSMSDFLWGLENSGWLRHIKAIMDAGIFIAKAVSEEGASVLVHCSDGWDRTAQVCS
VASLLLDPYYRTLKGFMVLIEKDWISFGHKFNHRYGNLDGDPKEISPVIDQFIECVWQLTEQFPCAFEFN
ERFLTHIQHHVYSCQFGNFLCNSQKERRELNENGKSKKGSRFLKEDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC032254
ORF Size 1401 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC032254, AAH32254
RefSeq Size 2817 bp
RefSeq ORF 1403 bp
Locus ID 54384
MW 53.9 kDa
Gene Summary Phosphatase that specifically dephosphorylates phosphatidylinositol 3-phosphate (PtdIns(3)P) and inositol 1,3-bisphosphate (Ins(1,3)P2).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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