Amigo1 (NM_001004293) Mouse Tagged ORF Clone

CAT#: MR207877

  • TrueORF®

Amigo1 (Myc-DDK-tagged) - Mouse adhesion molecule with Ig like domain 1 (Amigo1), transcript variant 2



  "NM_001004293" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


AMIGO1 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms ali2; Amigo
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207877 representing NM_001004293
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACCCCAGCGTGACCTGCGAGGCCTCTGGCTCCTGCTGCTCTCCGTGTTCCTGCTTCTCTTTGAGG
TAGCCAGGGCCGGCCGATCTGTGGTTAGTTGTCCCGCCAACTGCCTGTGCGCCAGCAACATCCTCAGCTG
CTCCAAGCAGCAGCTGCCCAATGTGCCCCAATCTTTGCCCAGCTACACAGCACTGCTGGACCTCAGCCAC
AACAACTTGAGCAGGCTGCGGGCCGAGTGGACCCCCACCCGGCTGACCAACCTGCACTCCCTGCTGCTGA
GCCACAACCACCTGAACTTCATCTCCTCCGAGGCCTTCGTCCCCGTACCCAACCTTAGGTACTTGGACCT
CTCCTCCAACCATCTTCACACGCTGGATGAGTTCCTGTTCAGCGACCTGCAGGCGCTGGAAGTGCTGTTG
CTCTACAATAACCACATTGTGGTGGTGGACCGGAATGCCTTTGAGGACATGGCCCAGCTGCAGAAACTCT
ACTTAAGCCAGAATCAGATCTCTCGCTTTCCTGTGGAACTGATCAAGGATGGGAACAAATTACCCAAACT
GATGCTCTTGGATCTGTCCTCCAACAAGCTGAAGAAGTTGCCCCTGACTGACCTGCAGAAATTGCCAGCC
TGGGTCAAGAATGGGCTATACCTGCATAACAACCCCTTGGAGTGCGACTGCAAGCTCTACCAGCTCTTTT
CGCACTGGCAGTACCGGCAGCTGAGCTCTGTGATGGACTTCCAGGAGGACCTGTACTGCATGCACTCCAA
GAAGCTGCACAACATCTTCAGCCTGGATTTCTTCAATTGCAGCGAGTACAAGGAAAGTGCCTGGGAGGCT
CACCTGGGAGACACCTTGACCATCAGGTGTGACACCAAACAGCAAGGCATGACCAAAGTGTGGGTGTCCC
CAAGCAATGAACAGGTGCTAAGTCAGGGGTCCAATGGCTCGGTGAGCGTGAGGAATGGCGACCTTTTTTT
TAAAAAGGTGCAGGTCGAGGATGGGGGTGTGTATACCTGTTACGCCATGGGGGAGACTTTCAACGAGACA
CTGTCTGTGGAGTTGAAAGTGTATAACTTCACCTTGCACGGACACCATGACACCCTCAACACAGCCTACA
CTACCCTGGTGGGCTGTATCCTCAGTGTGGTTCTGGTCCTCATATACTTGTACCTCACCCCTTGCCGCTG
CTGGTGTCGGGGTGTGGAGAAACCTTCCAGCCACCAAGGAGATAGCCTCAGCTCTTCTATGCTCAGTACC
ACACCCAACCACGACCCTATGGCTGGTGGGGACAAAGATGATGGTTTTGACCGGCGGGTGGCCTTCCTGG
AACCTGCTGGACCCGGGCAGGGTCAAAATGGCAAACTCAAGCCAGGCAACACTCTGCCGGTGCCCGAAGC
TACAGGCAAGGGCCAACGGAGGATGTCCGATCCAGAGTCGGTCAGCTCGGTCTTTTCTGATACACCCATT
GTGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207877 representing NM_001004293
Red=Cloning site Green=Tags(s)

MQPQRDLRGLWLLLLSVFLLLFEVARAGRSVVSCPANCLCASNILSCSKQQLPNVPQSLPSYTALLDLSH
NNLSRLRAEWTPTRLTNLHSLLLSHNHLNFISSEAFVPVPNLRYLDLSSNHLHTLDEFLFSDLQALEVLL
LYNNHIVVVDRNAFEDMAQLQKLYLSQNQISRFPVELIKDGNKLPKLMLLDLSSNKLKKLPLTDLQKLPA
WVKNGLYLHNNPLECDCKLYQLFSHWQYRQLSSVMDFQEDLYCMHSKKLHNIFSLDFFNCSEYKESAWEA
HLGDTLTIRCDTKQQGMTKVWVSPSNEQVLSQGSNGSVSVRNGDLFFKKVQVEDGGVYTCYAMGETFNET
LSVELKVYNFTLHGHHDTLNTAYTTLVGCILSVVLVLIYLYLTPCRCWCRGVEKPSSHQGDSLSSSMLST
TPNHDPMAGGDKDDGFDRRVAFLEPAGPGQGQNGKLKPGNTLPVPEATGKGQRRMSDPESVSSVFSDTPI
VV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001004293
ORF Size 1476 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001004293.2, NP_001004293.1
RefSeq Size 5016 bp
RefSeq ORF 1479 bp
Locus ID 229715
UniProt ID Q80ZD8
MW 55.8 kDa
Gene Summary Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain (By similarity). Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:22056818).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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