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Cnot6l (NM_144910) Mouse Tagged ORF Clone
CAT＃: MR207893
- TrueORF®
Cnot6l (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 6-like (Cnot6l), transcript variant 1
"NM_144910" in other vectors (3)
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CNY 4,104.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4932442K20Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207893 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAAGGAAAAATATGATCCTCCAGATCCTCGCAGAATTTACACCATCATGTCCGCAGAGGAGGTAG CCAATGGGAAAAAATCTCACTGGGCAGAGTTAGAGATCTCGGGTAGAGTGCGGAGCTTAAGTACATCACT CTGGTCATTAACACACTTGACAGCACTGCACCTAAATGACAATAACCTTGCTCGCATTCCACCTGATATT GCCAAGCTTCATAATCTGGTTTACCTGGATCTGTCATCCAATAAACTCAGAAGTTTACCAGCAGAACTAG GAAACATGGTGTCTCTCAGGGAATTGCTTTTAAATGACAATTATTTACGGGTGTTGCCTTATGAACTTGG CCGGCTCTTCCAGCTACAAACTCTAGGTTTAACAGGCAATCCTTTATCACAGGATATTATGAGCTTATAC CAGGACCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAATCTTGCAGTTCATCCAGAGCAGC TTCCTCCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTGCCATCAGCATCATTCACGGTTAT GTGTTACAATGTGTTATGTGATAAATATGCTACCAGGCAGCTATATGGCTATTGTCCGTCCTGGGCATTA AACTGGGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAACTGGGACGCAGATATCATTAGTCTTC AGGAAGTGGAAACAGAGCAATACTTTACTCTCTTTCTGCCAGCATTGAAGGATCGTGGATATGATGGATT TTTTTCTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAAAGAAAGCATGTGGATGGTTGTGCAATA TTCTTCAAAACAGAAAAATTTACATTGGTGCAGAAGCATACAGTGGAATTCAACCAGGTAGCAATGGCAA ATTCAGATGGATCCGAAGCAATGCTAAACAGAGTAATGACGAAAGATAACATTGGCGTTGCTGTGGTGTT AGAGGTCCACAAGGAGCTTTTTGGAACAGGTATGAAGCCTATTCATGCTGCAGACAAACAGCTGCTTATA GTGGCAAATGCCCACATGCATTGGGACCCAGAGTATTCTGATGTGAAACTTATTCAGACCATGATGTTTG TCTCAGAGGTTAAAAACATTCTGGAGAAAGCCTCAAGTAGGCCTGGCAGCCCAACTGCAGATCCCAATTC CATCCCGCTGGTGCTATGTGCAGATCTTAACTCATTGCCAGATTCAGGTGTTGTGGAATATTTAAGCAAC GGTGGAGTAGCTGACAACCATAAAGACTTCAAGGAACTAAGGTACAATGAGTGTCTTATGAACTTCAGCT GTAGTGGAAAGAATGGAAGCTCAGAAGGGAGAATCACACATGGCTTCCAACTTAAGAGCGCCTATGAAAA TAACTTGATGCCTTATACCAATTACACCTTTGATTTCAAAGGTGTGATTGACTACATTTTCTATTCCAAG ACTCATATGAACGTGCTTGGTGTCCTGGGGCCTTTAGATCCTCAATGGCTGGTTGAGAACAACATCACTG GGTGTCCACACCCTCACATCCCTTCAGACCACTTCTCACTGTTAACACAACTTGAACTCCACCCTCCACT CCTGCCTCTTGTCAATGGTGTTCACTTGCCTAATCGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207893 protein sequence
Red=Cloning site Green=Tags(s) MPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNNLARIPPDI AKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQDIMSLY QDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWAL NWEYRKKGIMEEIVNWDADIISLQEVETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHVDGCAI FFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAADKQLLI VANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSN GGVADNHKDFKELRYNECLMNFSCSGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSK THMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144910 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144910.2, NP_659159.1 |
| RefSeq Size | 8564 bp |
| RefSeq ORF | 1653 bp |
| Locus ID | 231464 |
| UniProt ID | Q8VEG6 |
| MW | 62.5 kDa |
| Gene Summary | Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218886 | Cnot6l (untagged) - Mouse CCR4-NOT transcription complex, subunit 6-like (Cnot6l), transcript variant 1, (10ug) |
CNY 7,030.00 |
|
| MR207893L3 | Lenti ORF clone of Cnot6l (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 6-like (Cnot6l), transcript variant 1 |
CNY 6,460.00 |
|
| MR207893L4 | Lenti ORF clone of Cnot6l (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 6-like (Cnot6l), transcript variant 1 |
CNY 6,460.00 |
