Arcn1 (NM_145985) Mouse Tagged ORF Clone
CAT#: MR208216
- TrueORF®
Arcn1 (Myc-DDK-tagged) - Mouse archain 1 (Arcn1)
ORF Plasmid: tGFP
"NM_145985" in other vectors (4)
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CNY 3,816.00
CNY 4,470.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4632432M07Rik; nur17 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208216 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGTTGGCAGCAGCAGTCTGCACGAAAGCGGGAAAGGCTATTGTTTCTCGACAGTTCGTGGAGA TGACCCGAACTCGGATTGAAGGCTTATTAGCAGCTTTCCCAAAACTCATGAACACTGGAAAACAACATAC TTTTGTTGAAACAGAGAGTGTAAGATATGTGTACCAACCTATGGAGAAACTGTACATGGTATTGATCACT ACAAAGAACAGCAATATCTTAGAAGATTTGGAGACTTTAAGGCTCTTCTCAAGGGTGATCCCTGAATATT GCCGAGCCTTAGAGGAAAATGAAATATCTGAGCACTGTTTTGATTTGATTTTTGCTTTTGATGAAATTGT TGCCCTGGGATACCGGGAGAATGTTAACCTGGCACAGATCAGAACCTTTACAGAAATGGACTCTCACGAG GAGAAAGTATTCCGAGCAGTCAGAGAGACTCAAGAACGTGAGGCTAAGGCTGAGATGCGGCGTAAAGCAA AGGAATTACAACAGGCCCGAAGAGATGCAGAGAGACAGGGGAAAAAAGCACCAGGATTTGGCGGATTTGG TAGTTCTGCAGTGTCTGGAGGCAGCACAGCAGCCATGATCACAGAGACTATCATTGAAACTGATAAACCA AAAGTGGCGCCTGCACCAGCCAGACCTTCAGGCCCCAGCAAGGCTTTGAAACTTGGAGCTAAAGGAAAGG AAGTAGATAACTTTGTGGACAAATTGAAATCTGAAGGTGAAACTATTATGTCTTCTAATATGGGAAAACG TACCTCAGAAGCAACCAAAGTGCATGCTCCACCTATTAATATGGAAAGTGTGCACATGAAGATTGAAGAG AAGATCACACTAACCTGTGGGAGAGATGGAGGATTACAGAATATGGAGTTGCATGGCATGACCATGCTTA GGATCTCAGATGACAAATTTGGCCGGATTCGTCTTCATGTAGAAAATGAAGATAAGAAAGGGGTGCAGCT ACAGACGCATCCAAATGTGGATAAAAAACTTTTTACTGCAGAGTCTCTCATTGGCTTGAAAAACCCAGAG AAGTCATTTCCAGTCAACAGTGATGTAGGGGTACTAAAGTGGAGACTACAAACAACAGAGGAATCTTTTA TTCCGTTGACAATTAATTGCTGGCCTTCGGAAAGTGGAAATGGCTGTGATGTCAACATAGAGTACGAACT ACAGGAAGATAATTTAGAGCTCAATGATGTGGTCATCACTATCCCACTCCCATCGGGTGTTGGCGCGCCT GTGATTGGTGAGATCGATGGGGAATATCGACATGACAGTCGGAGAAATACGTTGGAGTGGTGCCTGCCAG TGATTGATGCCAAAAATAAGAGTGGCAGCCTGGAGTTCAGCATTCCTGGGCAGCCTAATGACTTCTTCCC TGTTCAAGTTTCTTTCATCTCCAAAAAGAATTACTGCAACATACAGGTTACCAAAGTGACCCAGGTGGAT GGAAACAGCCCCGTCAGGTTTTCCACGGAGACCACTTTCTTAGTGGACAAATACGAAATCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208216 protein sequence
Red=Cloning site Green=Tags(s) MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPMEKLYMVLIT TKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHE EKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQGKKAPGFGGFGSSAVSGGSTAAMITETIIETDKP KVAPAPARPSGPSKALKLGAKGKEVDNFVDKLKSEGETIMSSNMGKRTSEATKVHAPPINMESVHMKIEE KITLTCGRDGGLQNMELHGMTMLRISDDKFGRIRLHVENEDKKGVQLQTHPNVDKKLFTAESLIGLKNPE KSFPVNSDVGVLKWRLQTTEESFIPLTINCWPSESGNGCDVNIEYELQEDNLELNDVVITIPLPSGVGAP VIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFSIPGQPNDFFPVQVSFISKKNYCNIQVTKVTQVD GNSPVRFSTETTFLVDKYEIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145985 |
ORF Size | 1536 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145985.3, NP_666097.2 |
RefSeq Size | 3470 bp |
RefSeq ORF | 1536 bp |
Locus ID | 213827 |
UniProt ID | Q5XJY5 |
MW | 57.2 kDa |
Gene Summary | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205817 | Arcn1 (untagged) - Mouse archain 1 (Arcn1), (10ug) |
CNY 3,816.00 |
|
MG208216 | Arcn1 (tGFP-tagged) - Mouse archain 1 (Arcn1) |
CNY 4,940.00 |
|
MR208216L3 | Lenti ORF clone of Arcn1 (Myc-DDK-tagged) - Mouse archain 1 (Arcn1) |
CNY 6,370.00 |
|
MR208216L4 | Lenti ORF clone of Arcn1 (mGFP-tagged) - Mouse archain 1 (Arcn1) |
CNY 6,370.00 |