United States
Germany
Japan
United Kingdom
China
Arhgef3 (BC005517) Mouse Tagged ORF Clone
CAT＃: MR208408
- TrueORF®
Arhgef3 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:7605 IMAGE:3494309)
ORF Plasmid: tGFP
"BC005517" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200004I24Rik; 9830169H03Rik; C76747; Xpln |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208408 representing BC005517
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCAAGGATTACCCCTTCTACCTCACTGTCAAGAGGGCAAACTGCAGCTTGGAGGCGCCCCTGG GCAGCGGGGTGGCCAAGGACGAGGAACCTAGTAACAAAAGGGTCAAACCCCTTTCCAGAGTCACATCGCT AGCAAACCTCATTCCACCTGTGAAGACCACACCATTAAAGCGCTTCAGCCAGACCTTGCAGCGGTCCATC AGCTTCCGCAGCGAGAGCCGCCCCGACATCCTTGCCCCCCGAGCTTGGTCCAGAAATGCCACCTCCTCAA GCACAAAGCGGAGAGACAGCAAACTGTGGAGTGAGACCTTCGATGTGTGTGTCAATCAGGTGCTCACGGC CAAGGAGATCAAGAGACAGGAGGCGATCTTTGAACTTTCTCAAGGTGAAGAAGACTTGATAGAGGACTTG AAATTAGCAAAAAAGGCCTACCACGACCCCATGCTGAAACTCTCCATAATGACGGAACAGGAACTGAATC AGATTTTTGGGACCCTGGACTCTCTAATTCCTCTCCATGAAGAGCTCCTCAGTCAGCTTCGAGATGTTCG GAAACCCGATGGCTCGACTGAGCACGTTGGTCCCATCCTCGTGGGCTGGCTGCCCTGTCTCAGCTCCTAC GACAGCTACTGCAGCAATCAAGTCGCCGCCAAAGCCCTGCTGGACCACAAAAAGCAGGACCACAGAGTGC AGGACTTCCTCCAGCGCTGTTTAGAATCACCCTTCAGTCGCAAACTCGATCTCTGGAATTTCCTTGATAT TCCCCGGAGCCGGCTAGTGAAGTATCCTCTGCTCCTTCGAGAAATCCTGAGGCATACACCAAATGATAAC CCAGACCAGCAGCACCTGGAAGAAGCTATAAACATCATCCAGGGAATTGTGGCAGAAATCAACACCAAGA CTGGGGAATCGGAATGCCGCTATTACAAAGAGCGGCTCCTTTACCTGGAAGAAGGCCAGAAAGATTCTCT GATTGACAGCTCAAGGGTCCTGTGTTGTCATGGAGAGCTGAAAAATAACCGGGGTGTGAAACTCCATGTT TTCCTGTTCCAAGAAGTGCTGGTGATCACCCGAGCTATCACTCACAACGAGCAGCTCTGCTATCAGTTGT ACCGGCAGCCCATCCCTGTGAAGGACCTCACGCTGGAAGACCTGCAGGATGGAGAAGTGCGGCTGGGTGG CTCCCTGCGCGGGGCCTTCAGCAACAACGAGAGAGTTAAAAACTTCTTCAGAGTCAGTTTCAAAAATGGA TCCCAAAGTCAGACCCACTCACTACAAGCCAACGACACCTTCAACAAACAGCAGTGGCTCAACTGTATCC GTCAGGCAAAAGAAACTGTTCTGTCTGCTGCCGGCCAGGCTGGCTTGCTTGACTCTGAGGGACTGGTCCA GGGTCCCGGCACTGAGAACAGGGAGCCACAGGGAGAAACAAAACTTGAGCAGATGGACCAATCGGACAGC GAGTCGGACTGCAGTATGGACACCAGTGAGGTCAGCCTTGAGTGTGAGCGGATGGAACAGACAGACGCTT CCTGTGCAAACAGCAGGCCCGAGGAGAGTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208408 representing BC005517
Red=Cloning site Green=Tags(s) MVAKDYPFYLTVKRANCSLEAPLGSGVAKDEEPSNKRVKPLSRVTSLANLIPPVKTTPLKRFSQTLQRSI SFRSESRPDILAPRAWSRNATSSSTKRRDSKLWSETFDVCVNQVLTAKEIKRQEAIFELSQGEEDLIEDL KLAKKAYHDPMLKLSIMTEQELNQIFGTLDSLIPLHEELLSQLRDVRKPDGSTEHVGPILVGWLPCLSSY DSYCSNQVAAKALLDHKKQDHRVQDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLLREILRHTPNDN PDQQHLEEAINIIQGIVAEINTKTGESECRYYKERLLYLEEGQKDSLIDSSRVLCCHGELKNNRGVKLHV FLFQEVLVITRAITHNEQLCYQLYRQPIPVKDLTLEDLQDGEVRLGGSLRGAFSNNERVKNFFRVSFKNG SQSQTHSLQANDTFNKQQWLNCIRQAKETVLSAAGQAGLLDSEGLVQGPGTENREPQGETKLEQMDQSDS ESDCSMDTSEVSLECERMEQTDASCANSRPEESV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC005517 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC005517.1 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 1574 bp |
| Locus ID | 71704 |
| MW | 133 kDa |
| Gene Summary | Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218430 | Arhgef3 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:7605 IMAGE:3494309), (10ug) |
CNY 6,940.00 |
|
| MG208408 | Arhgef3 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:7605 IMAGE:3494309) |
CNY 5,800.00 |
|
| MR208408L3 | Lenti ORF clone of Arhgef3 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:7605 IMAGE:3494309) |
CNY 7,130.00 |
|
| MR208408L4 | Lenti ORF clone of Arhgef3 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:7605 IMAGE:3494309) |
CNY 7,130.00 |
其它Arhgef3产品
