P4ha1 (NM_011030) Mouse Tagged ORF Clone
CAT#: MR208564
- TrueORF®
P4ha1 (Myc-DDK-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1)
ORF Plasmid: tGFP
"NM_011030" in other vectors (4)
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CNY 3,984.00
CNY 4,660.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AL022634; P4ha |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208564 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTGGGTTGTTTTAATGATGGCGATTCTACTTCCTCAGTCTTTAGCCCATCCAGGGTTTTTTACGT CAATTGGTCAGATGACTGACTTGATCCATAATGAGAAAGACCTGGTGACATCACTAAAAGATTATATTAA AGCAGAAGAGGACAAGTTAGAGCAAATCAAAAAATGGGCAGAGAAGTTAGACCGGCTAACGAGTACAGCA ACAAAAGACCCAGAGGGGTTTGTTGGGCATCCTGTAAACGCATTCAAGTTAATGAAACGTCTGAACACTG AGTGGAGTGAGTTGGAGAATCTGATCCTCAAGGACATGTCGGATGGCTTCATCTCTAACCTAACCATTCA GAGGCAGTACTTCCCTAATGATGAAGATCAGGTTGGAGCTGCTAAAGCTCTGTTTCGGCTGCAAGACACC TACAATCTGGACACAAATACCATCTCAAAGGGGAATCTTCCAGGAGTGCAACATAAGTCTTTCCTGACAG CCGAGGACTGCTTTGAGCTGGGCAAAGTGGCCTATACAGAGGCAGATTATTACCACACAGAACTGTGGAT GGAGCAGGCTCTGACGCAGCTGGAGGAGGGAGAGCTGTCTACTGTAGATAAAGTCTCTGTTCTGGATTAC CTGAGCTATGCGGTGTACCAGCAGGGAGACCTGGATAAGGCGCTTCTCCTCACCAAGAAGCTTCTTGAGC TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAGTATATTTTGAATATATAATGAGTAAAGAAAAAGA TGCCAATAAGTCAGCGTCGGGCGACCAATCTGATCAGAAAACTGCACCAAAGAAAAAAGGGATAGCTGTG GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACTCCCCGGA GACAGAAAAGACTGTTCTGCCGCTACCATGATGGGAACCGGAATCCTAAGTTCATCCTGGCCCCAGCCAA GCAGGAGGACGAGTGGGACAAGCCCCGGATTATCCGTTTCCATGACATCATCTCTGACGCGGAGATTGAG ATTGTCAAAGACTTAGCAAAACCAAGGCTGAGGCGAGCCACCATTTCAAACCCAGTAACAGGAGCCTTGG AGACGGTACATTACAGAATTAGCAAAAGTGCTTGGCTGTCTGGCTACGAAGACCCTGTGGTATCCCGAAT TAATATGAGAATACAAGATCTCACAGGACTGGATGTTTCTACGGCCGAGGAGTTACAGGTAGCAAATTAC GGAGTTGGAGGACAGTATGAGCCCCATTTTGACTTTGCAAGGAAAGATGAGCCGGATGCCTTTAGAGAGC TTGGGACAGGAAATAGGATTGCCACGTGGCTGTTCTATATGAGCGATGTGTCTGCTGGAGGAGCTACTGT TTTTCCTGAAGTGGGAGCCAGTGTTTGGCCCAAAAAAGGCACTGCTGTCTTCTGGTATAATCTGTTTGCC AGTGGGGAAGGAGATTACAGTACACGGCATGCAGCCTGTCCCGTGCTAGTTGGAAACAAATGGGTATCCA ACAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGGCCGTGCACCCTGTCAGAATTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208564 protein sequence
Red=Cloning site Green=Tags(s) MIWVVLMMAILLPQSLAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTA TKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDT YNLDTNTISKGNLPGVQHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALTQLEEGELSTVDKVSVLDY LSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTAPKKKGIAV DYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE IVKDLAKPRLRRATISNPVTGALETVHYRISKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANY GVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFA SGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011030 |
ORF Size | 1605 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011030.2, NP_035160.1 |
RefSeq Size | 4057 bp |
RefSeq ORF | 1605 bp |
Locus ID | 18451 |
UniProt ID | Q60715 |
MW | 60.9 kDa |
Gene Summary | Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217546 | P4ha1 (untagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1), (10ug) |
CNY 6,840.00 |
|
MG208564 | P4ha1 (tGFP-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1) |
CNY 5,130.00 |
|
MR208564L3 | Lenti ORF clone of P4ha1 (Myc-DDK-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1) |
CNY 6,560.00 |
|
MR208564L4 | Lenti ORF clone of P4ha1 (mGFP-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1) |
CNY 6,560.00 |