Fpgs (BC005484) Mouse Tagged ORF Clone

CAT#: MR208684

  • TrueORF®

Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (cDNA clone MGC:7296 IMAGE:3485401)

ORF Plasmid: DDK tGFP



  "BC005484" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,056.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AA408187
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208684 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTATCAGGATGCTGTGCGCACGCTCAACACCCTGCAAACCAATGCCAGCTACCTGGAGCAGGTAA
AGCGCCAACGGAGTGACCCCCAGGCGCAGCTGGAGGCTATGGAGATGTACCTGGCACGGAGTGGACTGCA
GGTGGAGGACTTGAACCGGCTAAACATTATTCATGTCACTGGGACCAAAGGGAAGGGCTCCACCTGTGCC
TTCACCGAACGGATCCTGCGGAATTACGGCCTGAAGACCGGCTTCTTTAGCTCTCCTCACATGGTGCAGG
TGCGGGAGCGGATTCGAATCAACGGGAAACCAATCAGCCCCGAGCTCTTCACCAAGCACTTCTGGTGCCT
CTATAACCAGCTGGAGGAGTTCAAGGACGACAGCCATGTCTCCATGCCCTCTTACTTCCGCTTCCTCACA
CTCATGGCCTTCCATGTCTTCCTCCAAGAGAAGGTGGACCTGGCAGTGGTGGAGGTGGGCATTGGCGGGG
CTTTTGACTGCACCAACATCATCAGAAAGCCAGTGGTGTGTGGAGTCTCCTCTCTTGGCATTGACCACAC
CAGTCTACTAGGAGATACAGTGGAGAAAATAGCATGGCAGAAAGGGGGCATCTTTAAGCCTGGTGTCCCT
GCCTTCACTGTGGTGCAGCCAGAAGGTCCCCTGGCTGTGCTGAGGGATCGAGCCCAGCAGATTGGATGCC
CGTTGTACCTGTGTCCGCCATTGGAAGCCCTGGAGGAGGTTGGACTGCCATTGAGCCTGGGTCTGGAGGG
AGCACACCAGCGGTCTAATGCTGCCTTGGCCTTGCAGCTGGCCCACTGTTGGCTGGAGCGGCAGGACCAC
CAAGACATCCAGGAGCTGAAGGTATCCAGGCCAAGCATACGGTGGCAGCTGCCCCTGGCACCTGTGTTCC
GCCCTACCCCTCACATGAGGCGTGGGCTTCGGGACACAGTGTGGCCTGGCCGGACACAGATACTCCAGCG
GGGACCCCTTACCTGGTACCTGGATGGCGCCCATACCACCAGCAGTGTGCAGGCCTGTGTGCACTGGTAC
CGCCAGTCATTGGAGCGCAGCAAACGCACCGATGGAGGGTCCGAAGTACACATCTTGCTCTTCAACTCTA
CTGGTGACAGGGACTCTGCTGCCCTGCTGAAGCTGCTGCAGCCCTGCCAGTTTGACTACGCTGTCTTCTG
CCCCAACGTGACAGAGGTTTCATCCATAGGAAATGCAGACCAGCAGAACTTCACTGTGACTCTGGACCAG
GTGCTGCTCCGCTGCCTCCAACACCAGCAGCATTGGAACGGCCTGGCTGAGAAACAGGCTAGCTCCAACC
TCTGGAGCAGCTGCGGCCCAGACCCTGCTGGGCCAGGCTCCCTGCTGCTGGCCCCGCACCCACCTCAGCC
TACTAGGACGAGCTCCCTCGTTTTCAGCTGCATCTCCCACGCCTTGCTGTGGATCAGCCAAGGCCGGGAT
CCCATCTTTCAGCCCCAGAGCCTTCCAAGGAATCTTCTCAACCACCCCACAGCCAACAGCGGGGCCAGCA
TTCTCCGTGAGGCTGCTGCCATCCATGTACTGGTTACAGGAAGCCTGCACCTGGTGGGCGGGGTTCTGAA
ACTGCTGGATCCCTCTATGTCCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208684 protein sequence
Red=Cloning site Green=Tags(s)

MEYQDAVRTLNTLQTNASYLEQVKRQRSDPQAQLEAMEMYLARSGLQVEDLNRLNIIHVTGTKGKGSTCA
FTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQLEEFKDDSHVSMPSYFRFLT
LMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVP
AFTVVQPEGPLAVLRDRAQQIGCPLYLCPPLEALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDH
QDIQELKVSRPSIRWQLPLAPVFRPTPHMRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWY
RQSLERSKRTDGGSEVHILLFNSTGDRDSAALLKLLQPCQFDYAVFCPNVTEVSSIGNADQQNFTVTLDQ
VLLRCLQHQQHWNGLAEKQASSNLWSSCGPDPAGPGSLLLAPHPPQPTRTSSLVFSCISHALLWISQGRD
PIFQPQSLPRNLLNHPTANSGASILREAAAIHVLVTGSLHLVGGVLKLLDPSMSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC005484
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC005484, AAH05484
RefSeq Size 2215 bp
RefSeq ORF 1637 bp
Locus ID 14287
MW 60.6 kDa
Gene Summary Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Dihydrofolate, tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate and 5-formyltetrahydrofolate are the best substrates. Folic acid and 5-methyltetrahydrofolate can also act as substrates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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