Elp3 (NM_028811) Mouse Tagged ORF Clone

CAT#: MR208702

  • TrueORF®

Elp3 (Myc-DDK-tagged) - Mouse elongation protein 3 homolog (S. cerevisiae) (Elp3)

ORF Plasmid: DDK tGFP



  "NM_028811" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,420.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2610507P14Rik; KAT9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208702 representing NM_028811
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCAAAAGAGGAAAGGGGACCTCAGCCCGGCTGAGCTGATGATGCTGACTATAGGAGACGTTATCA
AGCAGCTGGTTGAAGCTCACGAGCAGGGGAAAGACGTCGATCTAAATAAGATGAAAACCAAGACAGCTGC
CAAGTATGGCCTTGCATCCCAGCCCCGCCTGGTGGATATCATCGCTGCAGTCCCTCCTCACTATCGAAAG
ATCTTGATTCCAAAGCTAAAGGCGAAGCCCGTCAGGACTGCCAGTGGGATCGCTGTTGTGGCTGTGATGT
GCAAACCCCACAGATGTCCACACATCAGTTTCACAGGGAATATATGTATATACTGCCCCGGAGGACCTGA
CTCCGATTTTGAGTATTCAACCCAGTCTTACACGGGCTATGAGCCAACTTCCATGAGAGCTATCCGTGCT
AGATATGACCCTTTTCTGCAGACAAGACACCGGATAGAGCAGCTAAAGCAGCTTGGTCACAGTGTGGATA
AGGTGGAGTTCATCGTGATGGGTGGAACGTTCATGGCCCTTCCAGAAGAGTACAGAGATTATTTCATCCG
AAGTTTACACGATGCTTTATCAGGCCATACTTCAAACAATATTCACGAGGCAATCAAGTATTCTGAGAGA
AGCTTCACAAAGTGTGTTGGAATTACAATTGAGACCAGACCTGATTATTGCATGAAGCGGCACCTAAGTG
ACATGTTGACTTATGGCTGCACAAGGCTGGAGATCGGAGTGCAGAGTGTCTATGAAGATGTGGCCAGAGA
TACCAACAGGGGCCATACTGTGAAGGCAGCGTGTGAATCTTTCCACCTGGCCAAAGATTCTGGTTTCAAA
GTGGTGACGCATATGATGCCTGACCTGCCGAACGTGGGACTGGAGAGAGACATTGAGCAGTTTATAGAAT
TTTTCGAGAACCCTGCTTTTCGTCCTGATGGTTTGAAGCTCTACCCAACCCTGGTTATCCGTGGAACTGG
TCTGTATGAGCTGTGGAAATCAGGAAGATACAGGAGCTATTCTCCCAGTGACCTGATTGAGCTGGTGGCT
CGGATCCTAGCCCTTGTGCCCCCGTGGACTCGTGTGTACCGGGTGCAGAGAGACATCCCAATGCCCCTTG
TCAGCTCAGGAGTGGAGCATGGCAACCTGCGAGAGTTGGCATTTGCAAGAATGAAGGATCTTGGAATACA
GTGCCGTGATGTGAGAACCAGGGAGGTTGGAATCCAAGAAATTCATCACAGAGTGCGGCCATACCAGGTC
GAGTTGGTACGGAGAGATTATGTTGCCAATGGTGGCTGGGAAACTTTCCTGTCCTACGAAGATCCTGATC
AGGACATTCTAATTGGCCTCCTAAGGCTGCGGAAGTGCTCTGAGGAAACCTTCCGGTTTGAACTGGGTGG
AGGTGTTTCCATAGTCCGAGAGCTACACGTGTATGGAAGTGTGGTCCCTGTGAGCAGCCGGGATCCCACT
AAATTTCAGCATCAGGGGTTCGGCATGCTGCTGATGGAAGAGGCAGAGAGAATCGCTCGGGAAGAGCATG
GATCTGGGAAAATGGCAGTTATATCAGGGGTCGGCACAAGAAATTACTACCGAAAGATTGGCTACAGATT
ACAAGGCCCCTATATGGTAAAGATGCTAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208702 representing NM_028811
Red=Cloning site Green=Tags(s)

MRQKRKGDLSPAELMMLTIGDVIKQLVEAHEQGKDVDLNKMKTKTAAKYGLASQPRLVDIIAAVPPHYRK
ILIPKLKAKPVRTASGIAVVAVMCKPHRCPHISFTGNICIYCPGGPDSDFEYSTQSYTGYEPTSMRAIRA
RYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRSLHDALSGHTSNNIHEAIKYSER
SFTKCVGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAACESFHLAKDSGFK
VVTHMMPDLPNVGLERDIEQFIEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYRSYSPSDLIELVA
RILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELAFARMKDLGIQCRDVRTREVGIQEIHHRVRPYQV
ELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPT
KFQHQGFGMLLMEEAERIAREEHGSGKMAVISGVGTRNYYRKIGYRLQGPYMVKMLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028811
ORF Size 1641 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028811.1, NM_028811.2, NM_028811.3, NP_083087.1
RefSeq Size 2724 bp
RefSeq ORF 1644 bp
Locus ID 74195
UniProt ID Q9CZX0
MW 62.8 kDa
Gene Summary Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Involved in acetylation of alpha-tubulin (By similarity). May also have a methyltransferase activity. Involved in cell migration. Involved in neurogenesis. Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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