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Mapk15 (NM_177922) Mouse Tagged ORF Clone
CAT＃: MR208733
- TrueORF®
Mapk15 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 15 (Mapk15)
ORF Plasmid: tGFP
"NM_177922" in other vectors (3)
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CNY 4,096.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC048082 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208733 representing NM_177922
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGCTGCCGAGGTGGACCGTCATGTAGCCCAGAGATACCTGATCAAGCGGAGGCTTGGGAAGGGGG CCTATGGCATTGTGTGGAAGGCCATGGACCGGAGGACTGGCGAGGTTGTGGCCATCAAGAAAATCTTTGA TGCCTTTAGGGACCAGATAGATGCTCAGAGGACCTTCCGTGAGATTATGCTTCTCAAGGAGTTTGGAGGC CATCCCAACATCATCCGCCTGCTTGATGTAATCCCAGCGAAGAATGACAGGGATATTTACCTGGTGTTTG AGTCCATGGACACCGACCTGAATGCAGTCATCCAGAAGGGCAGACTACTGAAGGACATCCACAAGCGGTG CATCTTTTACCAGCTCCTGAGAGCCACCAAGTTTATCCATTCAGGGCGCGTCATCCATCGGGATCAGAAG CCAGCCAATGTTCTACTGGATTCTGCTTGCCGGGTGAAACTCTGTGACTTTGGCCTGGCACGCTCCCTCG GTGACCTCCCTGAGGGGCCTGGGGGTCAGGCCCTGACAGAGTATGTGGCCACACGCTGGTACCGAGCTCC AGAGGTGCTTCTGTCTTCCCGATGGTATACCCCTGGGGTGGACATGTGGAGCCTGGGCTGCATATTAGGA GAGATGCTTCGAGGGCAGCCACTGTTTCCCGGGACATCTACTTTCCACCAGCTGGAGCTGATCCTGAAGA CCATCCCATTGCCTTCCATGGAGGAGCTCCAGGACCTTGGCTCAGACTACAGTGCTTTGATTCTGCAGAA TCTTGGGTCCAGGCCACAGCAGACGCTGGACGCCCTCCTGCCGCCAGACACCCCCCCAGAAGCCCTGGAC CTCCTCAAGCGACTCTTGGCGTTTGCTCCGGACAAGCGCCTTAGTGCAGAGCAGGCGCTGCAACACCCCT ACGTGCAGAGATTCCATTGCCCCGACCGCGAGTGGGCACGGGAGTCCGACGTGCGGCTCCCGGTGCACGA AGGAGACCAGCTCTCTGCACCAGAGTATCGCAAACGCCTGTACCAGATTATCCTGGAGCAAAGTGGGAAC AGCCGCAGCCCTCGAGAGGAAGGCCTGGGGGTTGTGGCCTCGCGGGCTGAGCTCAGGGCTTCCCCGGCCC GGACGCAATCGCTCAAGTCGGGAGTCCTCCCCCAGGTCCCGGCGGAGACGCCAGCGCGAAAACGCGGACC CAAACCTCCGCGTAGCCCTGGTCATGATCCTGAGCATGTGGAAGTTCGCAGGCAGAGCTCAGACCCCCTG TTCCAACTTCCGCCGCCAGGAAGGGGGGAAAGGCCCCCAGGGGCCACAGGGCAGCCACCCTCGGCACCCT CAGGGGTGAAGACTCAAGTGAGGGCGATGGCGCCGTCCCTGACTTCACAGGCAGAGGCTCAGGCGGCCAA TCAGGCTCTGATCCGCAGTGATCCGGCCCGGGGCGGTGGGCCGAGGGCGGTCGGCGCGCGACGGGTCCCT TCCCGCCTGCCCCGGGAGGCCCCGGAACCCCGACCCGGCCGAAGGATGTTTGGCATCTCGGTCTCGCAGG GGGCCCAGGGCGCAGCCAGAGCTGCGCTTGGCGGCTACTCCCAGGCCTACGGGACCGTGTGCCGCTCGGC GCTGGGCCGCCTGCCCCTGCTCCCCGG[AT]CCGCGTGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208733 representing NM_177922
Red=Cloning site Green=Tags(s) MCAAEVDRHVAQRYLIKRRLGKGAYGIVWKAMDRRTGEVVAIKKIFDAFRDQIDAQRTFREIMLLKEFGG HPNIIRLLDVIPAKNDRDIYLVFESMDTDLNAVIQKGRLLKDIHKRCIFYQLLRATKFIHSGRVIHRDQK PANVLLDSACRVKLCDFGLARSLGDLPEGPGGQALTEYVATRWYRAPEVLLSSRWYTPGVDMWSLGCILG EMLRGQPLFPGTSTFHQLELILKTIPLPSMEELQDLGSDYSALILQNLGSRPQQTLDALLPPDTPPEALD LLKRLLAFAPDKRLSAEQALQHPYVQRFHCPDREWARESDVRLPVHEGDQLSAPEYRKRLYQIILEQSGN SRSPREEGLGVVASRAELRASPARTQSLKSGVLPQVPAETPARKRGPKPPRSPGHDPEHVEVRRQSSDPL FQLPPPGRGERPPGATGQPPSAPSGVKTQVRAMAPSLTSQAEAQAANQALIRSDPARGGGPRAVGARRVP SRLPREAPEPRPGRRMFGISVSQGAQGAARAALGGYSQAYGTVCRSALGRLPLLPXXPRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177922 |
| ORF Size | 1650 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177922.1, NM_177922.2, NP_808590.1 |
| RefSeq Size | 1961 bp |
| RefSeq ORF | 1650 bp |
| Locus ID | 332110 |
| UniProt ID | Q80Y86 |
| MW | 61.1 kDa |
| Gene Summary | Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (By similarity) (PubMed:25823377). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3' UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (By similarity). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (By similarity). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (PubMed:23351492). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208733 | Mapk15 (tGFP-tagged) - Mouse mitogen-activated protein kinase 15 (Mapk15) |
CNY 5,040.00 |
|
| MR208733L3 | Lenti ORF clone of Mapk15 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 15 (Mapk15) |
CNY 6,460.00 |
|
| MR208733L4 | Lenti ORF clone of Mapk15 (mGFP-tagged) - Mouse mitogen-activated protein kinase 15 (Mapk15) |
CNY 6,460.00 |
