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Tnk2 (BC031168) Mouse Tagged ORF Clone
CAT＃: MR208742
- TrueORF®
Tnk2 (Myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (cDNA clone MGC:37479 IMAGE:4984168)
ORF Plasmid: tGFP
"BC031168" in other vectors (4)
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CNY 5,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ack, Cdgip, ACK1, MGC37479 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208742 representing BC031168
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGA AAAAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCT GAGGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCA GACCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGC CCTGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAG CCCCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCC CCGCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGC TAGTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGC GCAGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCA GTGCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC AGCTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC TCGGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCT GGACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCC CCCTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCAC CACTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCA GGCCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTG AGCGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGC CCTGCCCGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGA CCTATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGC CACCATCCCTGAGGGCCGCGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCG GCCAGCAGACAAGGTCCAGATGCTGCAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCC CTGCAGAGCCACAGCTGGAGTGTTCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTTGGGCTGG GTCTTCGGCCACGGGTGGAGTGCCACAAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTG TCACCTTCTGGGCTCCTGTGGCCCTGCTCATCACAAGCGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR208742 representing BC031168
Red=Cloning site Green=Tags(s) MDPPDLLSVELSTSRPTQHLGRVKKPTYDPVSEDPDPLSSDFKRLGLRKPALPRGLWLAKPSARVPGTKA DRSSGGEVTLIDFGEEPVVPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRPLHPTPVVDWDARPLP PPPAYDDVAQDEDDFEVCSINSTLVGAGLPAGPSQGETNYAFVPEQAQMPPALEDNLFLPPQGGGKPPSS VQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVELSPAPSGEEETSRWP GPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRA GPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLPPPSTPAPAAPTATVR PMPQAAPDPKANFSTNNSNPGARPPSLRAAARLPQRGCPGDGQEAARPADKVQMLQAMVHGVTTEECQAA LQSHSWSVQRAAQYLKVEQLFGLGLRPRVECHKVLEMFDWNLEQAGCHLLGSCGPAHHKR SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031168 |
| ORF Size | 1650 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031168.1 |
| RefSeq Size | 2823 bp |
| RefSeq ORF | 1652 bp |
| Locus ID | 51789 |
| MW | 103.5 kDa |
| Gene Summary | Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218302 | Tnk2 (untagged) - Mouse tyrosine kinase, non-receptor, 2 (cDNA clone MGC:37479 IMAGE:4984168), (10ug) |
CNY 6,940.00 |
|
| MG208742 | Tnk2 (tGFP-tagged) - Mouse tyrosine kinase, non-receptor, 2 (cDNA clone MGC:37479 IMAGE:4984168) |
CNY 6,080.00 |
|
| MR208742L3 | Lenti ORF clone of Tnk2 (Myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (cDNA clone MGC:37479 IMAGE:4984168) |
CNY 7,410.00 |
|
| MR208742L4 | Lenti ORF clone of Tnk2 (mGFP-tagged) - Mouse tyrosine kinase, non-receptor, 2 (cDNA clone MGC:37479 IMAGE:4984168) |
CNY 7,410.00 |
