Dnajc1 (BC080300) Mouse Tagged ORF Clone

CAT#: MR208765

  • TrueORF®

Dnajc1 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 1 (cDNA clone MGC:90686 IMAGE:30536910)

ORF Plasmid: DDK tGFP



  "BC080300" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MTJ1, ERdj1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208765 representing BC080300
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGTTCCCGGCTTCGGATCCGCGCGACTTCCCCAGCGCCGGCGCTCCGGGTTGGAGTCATCCTCGG
TGCGGCCGCTGTGGCTGCTGCTGCTGTTCCTGCTGGCAGCCGTGCGGCCAGTGCGCGCCTGGGAGAGCGG
AGACCTGGAGTTGTTCGACTTGGTGGAAGAGGTGCAGTTGAACTTCTACGAGTTCCTCGGGGTGCAGCAG
GATGCTTCATCTGCAGACATCAGAAAAGCATATCGTAAGCTTTCACTAACCTTACATCCAGACAAGAATA
AAGATGAAAATGCAGAAACTCAATTTAGACAATTGGTGGCCATTTATGAAGTTTTAAAGGATGATGAAAG
AAGGCAGAGGTATGATGATGTTCTGATCAATGGACTTCCAGATTGGCGACAGCCTGTATTCTACTACAGG
CGAGTGAGAAAAATGAGCAATGCTGAGCTGGCTTTGCTCTTGTTCATTATTCTCACAGTGGGTCACTATG
CTGTGGTTTGGTCCATCTACCTGGAAAAGCAACTGGATGAACTGCTTGGTAGAAAAAAGAGAGAAAGGAA
GAAGAAGACAGGAAGCAAGAGTGTGGACGCAGCAAAACTTGGTGCTTCTGAAAAGAACGAAAGATTGCTT
ATAAAACCACAATGGCATGATTTGCTTCCATGCAAACTGGGGATTTGGTTTTGCCTTACACTAAAAGCAT
TGCCTCATCTAATCCAGGATGCTGGGCAGTTTTATGCAAAATATAAGGAGACAAAATTGAAGGAAAAAGA
AGACGCACTGGCTAGAATTGAAATTGAAACCCTTCAAAAACAGAAGAAAGTTAAAGTTAAAAAACCAAAA
CCAGAATTTCCTGTGTATATGCCTTTAGAAAATACGTATATTCAGTCTTATGATCATGGAACTTCTATTG
AAGAAATTGAGGAGCAAATGGATGACTGGCTGGAAAACAGGAAAAGAACACAGAAAAGACAGGCACCTGA
ATGGACAGAAGAGGACCTCAGTCAACTGACAAGAAGTATGGTTAAGTTCCCAGGAGGGACTCCAGGTCGA
TGGGACAAGATTGCCCATGAATTGGGTCGATCTGTGACAGATGTGACAACCAAAGCCAAGGAACTGAAGG
ACTCAGTCACCAGCTCCCCCGGAATGACCAGGCTCTCTGAACTTAAGTCAAATGGTCAGAATTCCAGGCC
AATCAAGATAGCCACAGCCTTGCCAGATGACATCATCACGCAGCGGGAGGACTCAGCAGGGGCCATGGAG
GATGAGGAGCACGAGGCTGCTGAGGGTGAGCAGGAGTCAGCGACCACAGAAGCCCGACCTCGGAGGCGGA
AGTCAGCCAGGGCGGCTGAGGCTGTAACCAGAGTGGAGCCAGAGGAGAAGCTGAGAGGAAAGAGGCAAAA
AGACTTTGATATATCAGAACAAAACGATTCCAGTGACGAAGAGAAACAACGAAAAGAAAGAACTCGTGCT
GCAGAGGAGGCGTGGACTCAGAGTCAGCAGAAACTTCTGGAATTGGCATTACAGCAATACCCTAAAGGAG
CTTCTGACCGCTGGGACAAAATAGCCAAATGTGTCCCGTCTAAGAGTAAGGAAGACTGCATTGCTAGATA
CAAGCTGCTGGTTGAACTGGTCCAAAAGAAAAAGCAGGCTAAAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208765 representing BC080300
Red=Cloning site Green=Tags(s)

MWVPGFGSARLPQRRRSGLESSSVRPLWLLLLFLLAAVRPVRAWESGDLELFDLVEEVQLNFYEFLGVQQ
DASSADIRKAYRKLSLTLHPDKNKDENAETQFRQLVAIYEVLKDDERRQRYDDVLINGLPDWRQPVFYYR
RVRKMSNAELALLLFIILTVGHYAVVWSIYLEKQLDELLGRKKRERKKKTGSKSVDAAKLGASEKNERLL
IKPQWHDLLPCKLGIWFCLTLKALPHLIQDAGQFYAKYKETKLKEKEDALARIEIETLQKQKKVKVKKPK
PEFPVYMPLENTYIQSYDHGTSIEEIEEQMDDWLENRKRTQKRQAPEWTEEDLSQLTRSMVKFPGGTPGR
WDKIAHELGRSVTDVTTKAKELKDSVTSSPGMTRLSELKSNGQNSRPIKIATALPDDIITQREDSAGAME
DEEHEAAEGEQESATTEARPRRRKSARAAEAVTRVEPEEKLRGKRQKDFDISEQNDSSDEEKQRKERTRA
AEEAWTQSQQKLLELALQQYPKGASDRWDKIAKCVPSKSKEDCIARYKLLVELVQKKKQAKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC080300
ORF Size 1656 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC080300
RefSeq Size 5087 bp
RefSeq ORF 1658 bp
Locus ID 13418
MW 186.5 kDa
Gene Summary May modulate protein synthesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...