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Plat (NM_008872) Mouse Tagged ORF Clone
CAT＃: MR208868
- TrueORF®
Plat (Myc-DDK-tagged) - Mouse plasminogen activator, tissue (Plat)
ORF Plasmid: tGFP
"NM_008872" in other vectors (3)
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CNY 4,168.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU020998; AW212668; D8Ertd2; D8Ertd2e; t; t-; tPA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208868 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGAGAGCTGCTGTGTGTACTGCTGCTTTGTGGACTGGCTTTCCCATTGCCTGACCAGGGAATAC ATGGGAGGTTCAGAAGAGGAGCCCGGTCCTACAGAGCGACCTGCAGAGATGAGCCAACGCAGACAACTTA CCAACAGCACCAGTCGTGGCTCCGACCCATGCTCAGAAGCAGCCGGGTGGAATATTGCCGGTGCAACAGC GGCCTGGTACAATGCCACTCAGTGCCTGTCCGAAGTTGCAGCGAACCAAGATGCTTCAATGGGGGGACGT GTCAGCAGGCCCTGTATTTCTCTGACTTTGTCTGCCAGTGCCCTGATGGATTTGTAGGGAAACGCTGTGA CATAGATACCAGAGCAACATGCTTTGAGGAGCAGGGCATCACCTACAGAGGCACGTGGAGCACAGCAGAA AGTGGGGCTGAGTGCATCAACTGGAATAGCAGTGTTCTGTCGCTGAAGCCCTACAATGCAAGGAGGCCAA ATGCCATCAAGCTGGGCCTTGGGAATCACAATTACTGCAGAAACCCAGACCGAGACTTGAAGCCCTGGTG CTATGTCTTTAAGGCAGGGAAGTATACCACGGAGTTCTGTAGCACACCAGCTTGCCCTAAGGGAAAAAGC GAGGACTGCTATGTTGGGAAAGGTGTGACTTACCGTGGCACCCACAGCCTCACCACATCCCAGGCCTCCT GCCTCCCCTGGAATTCCATAGTCCTCATGGGCAAGAGTTACACAGCGTGGAGGACCAACTCCCAGGCACT CGGCCTGGGCAGACACAATTATTGTCGGAATCCAGATGGTGATGCCAGACCTTGGTGCCATGTGATGAAG GACCGAAAGCTGACGTGGGAATACTGTGACATGTCCCCATGCTCCACCTGTGGCCTGAGGCAGTACAAAC GGCCTCAGTTTAGAATTAAAGGAGGACTCTACACAGACATCACCTCACACCCTTGGCAGGCTCCCATCTT TGTCAAGAACAAGAGGTCTCCTGGAGAGAGATTCCTTTGTGGAGGGGTGCTGATCAGTTCCTGCTGGGTG CTGTCAGCTGCCCACTGCTTTCTAGAGAGGTTCCCCCCCAATCATCTTAAAGTGGTCTTGGGCAGAACAT ACAGGGTGGTCCCCGGAGAGGAAGAACAGACATTTGAGATTGAAAAATACATAGTCCATGAGGAATTTGA TGACGATACTTATGACAACGACATCGCATTACTGCAGCTGAGGTCACAGTCCAAGCAATGTGCCCAAGAG AGCAGCTCTGTTGGCACTGCCTGCCTCCCTGACCCCAACCTGCAGCTCCCTGACTGGACAGAGTGTGAGC TTTCTGGCTACGGCAAGCATGAGGCATCGTCTCCATTCTTCTCTGACCGGCTGAAGGAGGCTCACGTCAG ACTGTACCCGTCCAGCCGCTGCACCTCACAGCATCTGTTTAATAAAACCGTCACGAACAACATGCTGTGC GCTGGAGACACCCGGAGCGGAGGCAACCAAGACCTCCACGATGCATGCCAGGGTGACTCGGGAGGCCCTC TGGTGTGCATGATCAATAAACAGATGACATTGACTGGCATTATCAGCTGGGGCCTCGGCTGTGGGCAGAA GGATGTGCCTGGGGTCTACACAAAGGTTACGAATTACCTAGACTGGATTCACGACAACATGAAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208868 protein sequence
Red=Cloning site Green=Tags(s) MKRELLCVLLLCGLAFPLPDQGIHGRFRRGARSYRATCRDEPTQTTYQQHQSWLRPMLRSSRVEYCRCNS GLVQCHSVPVRSCSEPRCFNGGTCQQALYFSDFVCQCPDGFVGKRCDIDTRATCFEEQGITYRGTWSTAE SGAECINWNSSVLSLKPYNARRPNAIKLGLGNHNYCRNPDRDLKPWCYVFKAGKYTTEFCSTPACPKGKS EDCYVGKGVTYRGTHSLTTSQASCLPWNSIVLMGKSYTAWRTNSQALGLGRHNYCRNPDGDARPWCHVMK DRKLTWEYCDMSPCSTCGLRQYKRPQFRIKGGLYTDITSHPWQAPIFVKNKRSPGERFLCGGVLISSCWV LSAAHCFLERFPPNHLKVVLGRTYRVVPGEEEQTFEIEKYIVHEEFDDDTYDNDIALLQLRSQSKQCAQE SSSVGTACLPDPNLQLPDWTECELSGYGKHEASSPFFSDRLKEAHVRLYPSSRCTSQHLFNKTVTNNMLC AGDTRSGGNQDLHDACQGDSGGPLVCMINKQMTLTGIISWGLGCGQKDVPGVYTKVTNYLDWIHDNMKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008872 |
| ORF Size | 1680 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008872.1, NM_008872.2, NM_008872.3, NP_032898.1 |
| RefSeq Size | 2548 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 18791 |
| UniProt ID | P11214 |
| MW | 63.1 kDa |
| Gene Summary | This gene encodes a key enzyme of the fibrinolytic pathway. The encoded protein undergoes proteolytic processing by plasmin to generate a heterodimeric serine protease that cleaves the proenzyme plasminogen to produce plasmin, a protease that is required to break down fibrin clots. Additionally, the encoded protein is involved in other biological processes such as synaptic plasticity, cell migration and tissue remodeling. Mice lacking the encoded protein display a reduction in long-term potentiation in hippocampus and conversely, transgenic mice overexpressing the encoded protein have increased and prolonged long-term potentiation. [provided by RefSeq, Jul 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airway
,Harbig, A;Mernberger, M;Bittel, L;Pleschka, S;Schughart, K;Steinmetzer, T;Stiewe, T;Nist, A;Böttcher-Friebertshäuser, E;,
J. Biol. Chem.
,PubMed ID 32303635
[PLAT]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208868 | Plat (tGFP-tagged) - Mouse plasminogen activator, tissue (Plat) |
CNY 5,040.00 |
|
| MR208868L3 | Lenti ORF clone of Plat (Myc-DDK-tagged) - Mouse plasminogen activator, tissue (Plat) |
CNY 6,460.00 |
|
| MR208868L4 | Lenti ORF clone of Plat (mGFP-tagged) - Mouse plasminogen activator, tissue (Plat) |
CNY 6,460.00 |
