Zmynd11 (BC022945) Mouse Tagged ORF Clone

CAT#: MR208906

  • TrueORF®

Zmynd11 (Myc-DDK-tagged) - Mouse zinc finger, MYND domain containing 11 (cDNA clone MGC:30718 IMAGE:3982190)

ORF Plasmid: DDK tGFP



  "BC022945" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2210402G22Rik; BS69
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208906 representing BC022945
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGAGTCCACGGTATGCATCCCAAAGAGACCACCCGCCAGCTGAGCTTAGCTGTGAAAGATGGTC
TTATTGTCGAAACTCTAACAGTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAGGAAGGATATTGGTT
ACCAGGAGATGAGATTGACTGGGAAACAGAAACTCATGACTGGTATTGTTTTGAATGCCATTTGCCTGGA
GAGGTGTTGATATGTGACCTGTGTTTTCGTGTGTATCATTCCAAGTGTTTGTCTGATGAGTTCAGGCTTA
GAGACAGCAGTAGTCACTGGCAGTGCCCAGTTTGCAGGAGCATTAAGAAGAAGCACTCTAACAAGCAGGA
AATGGGCACCTACTTGAGGTTCATCGTCTCCCGCATGAAGGAGCGGGCTATAGACCTTAATAAGAAAGGA
AAGGACAGTAAGCATCCTATGTACCGGAGGCTGGTCCACTCAGCTGTTGACGTCCCTACCATACAAGAGA
AGGTGAATGAGGGGAAGTACCGGAGTTACGAGGAGTTCAAGGCTGACGCACAGCTGCTTCTCCACAACAC
AGTGATCTTCTATGGAGCAGACAGTGAGCAAGCGGACATTGCACGGATGTTATATAAAGACACGTGTCAC
GAGCTGGACGAGCTGCAGCTTTGCAAGAACTGCTTCTATCTGTCAAACGCACGGCCCGACAACTGGTTCT
GCTACCCTTGCATACCTAATCATGAGCTGGTTTGGGCTAAAATGAAAGGTTTTGGGTTTTGGCCAGCCAA
AGTCATGCAGAAAGAAGACAATCAAGTGGATGTTCGCTTCTTTGGCCATCACCACCAGAGAGCCTGGATT
CCTTCTGAAAACATCCAGGATATCACGGTCAATGTTCACCGACTGCACGTGAAGCGCAGCATGGGCTGGA
AGAAGGCCTGTGACGAGCTGGAACTGCATCAGCGCTTCCTCCGTGAAGGAAGGTTCTGGAAGTCTAAGAA
TGAGGACCGAGGTGAGGAAGAGGCAGAATCCAGTATCTCCTCCACCAGTAACGAGCAGCTGAAGGTCACT
CAAGAGCCAAGAGCAAAGAAAGGACGACGTAATCAGAGTGTGGAGCCCAAAAAGGAAGAACCCGAGCCAG
AAACGGAAGCAGTAAGTTCCAGCCAGGAAATCCCCACAATGCCTCAGCCAATTGAGAGGGTGTCAGTGTC
AACCCAGACCAAGAAGTTAAGTGCCTCTTCCCCACGAATGCTGCATCGAAGCACTCAGACGACGAGCGAC
GGCGTCTGTCAGAGCATGTGCCACGACAAGTACACCAAAATTTTTAATGACTTTAAAGACAGGATGAAGT
CGGACCACAAGCGTGAAACAGAGAGAGTGGTCCGTGAAGCGCTGGAAAAGTTGCGTTCTGAAATGGAAGA
AGAAAAAAGACAAGCCGTAAACAAAGCTGTGGCCAACCTGCAGGGCGAGATGGACAGAAAGTGTAAGCAG
GTGAAGGAAAAGTGCAAGGAAGAATTTGTAGAGGAGATCAAGAAGCTAGCGGCGCAGCACAAGCAGCTCA
TTTCTCAGACCAAGAAGAAGCAGTGGTGCTACAACTGTGAGGAGGAGGCCATGTACCACTGCTGCTGGAA
CACGTCCTACTGCTCCATCAAGTGCCAGCAAGAGCACTGGCACGCAGAGCACAAGCGCACCTGCCGCCGG
AAGAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208906 representing BC022945
Red=Cloning site Green=Tags(s)

MSRVHGMHPKETTRQLSLAVKDGLIVETLTVGCKGSKAGIEQEGYWLPGDEIDWETETHDWYCFECHLPG
EVLICDLCFRVYHSKCLSDEFRLRDSSSHWQCPVCRSIKKKHSNKQEMGTYLRFIVSRMKERAIDLNKKG
KDSKHPMYRRLVHSAVDVPTIQEKVNEGKYRSYEEFKADAQLLLHNTVIFYGADSEQADIARMLYKDTCH
ELDELQLCKNCFYLSNARPDNWFCYPCIPNHELVWAKMKGFGFWPAKVMQKEDNQVDVRFFGHHHQRAWI
PSENIQDITVNVHRLHVKRSMGWKKACDELELHQRFLREGRFWKSKNEDRGEEEAESSISSTSNEQLKVT
QEPRAKKGRRNQSVEPKKEEPEPETEAVSSSQEIPTMPQPIERVSVSTQTKKLSASSPRMLHRSTQTTSD
GVCQSMCHDKYTKIFNDFKDRMKSDHKRETERVVREALEKLRSEMEEEKRQAVNKAVANLQGEMDRKCKQ
VKEKCKEEFVEEIKKLAAQHKQLISQTKKKQWCYNCEEEAMYHCCWNTSYCSIKCQQEHWHAEHKRTCRR
KR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC022945
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC022945.1
RefSeq Size 3011 bp
RefSeq ORF 1688 bp
Locus ID 66505
MW 110.3 kDa
Gene Summary Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage (PubMed:24590075). Binds non-specifically to dsDNA (By similarity). Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth (PubMed:24590075).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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