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Lmf1 (NM_029624) Mouse Tagged ORF Clone
CAT＃: MR208947
- TrueORF®
Lmf1 (Myc-DDK-tagged) - Mouse lipase maturation factor 1 (Lmf1)
ORF Plasmid: tGFP
"NM_029624" in other vectors (4)
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CNY 4,232.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2400010G15Rik; AW822050; cld; Tmem112 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208947 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGCCCGAGGGGTCGCTGAGAAAACGGAAGGTCGGGGGTGCGGAGCACAGTCCAGCGTCGCAAC CTTCTCTGGCTAGAGATCCCGCAGATTCTCCAGCTCGGCTGCACACGGGCACCTTCTGGCTGACTAGAAT CGTGCTCCTGCGGGCCCTCGCTTTCATTTACTTTGTGGCCTTTTTGGTGGCATTCAATCAGAACAAGGCC CTCATCGGTGACCGGGGCCTGCTGCCCTGCAAACTGTACCTGAAGAATGTCCAGGAGTACTTCCAGGGCA GCACTGGCTGGGCTGCCTGGACCTATGCACCAACCATTATGTGGTTGTTGGACTGGTCAGACATGAACTT CAACCTGGACCTCATTGCTCTTCTTGGGCTGGGCATCTCGTCCTTTGTCCTGGTGACCGGCTGTGCCAAC ATGATACTCATGACTGCCCTCTGGGCCCTCTACATGTCCCTGGTCAACGTAGGGCAGATCTGGTATTCTT TTGGATGGGAGTCACAGCTGCTGGAAACAGGATTCCTGGGGATCTTTCTGAGCCCTCTCTGGACACTGTC CCGCCTGCCCAAGAACACCCCCACATCCCAGATTGTCCTGTGGGGCTTCCGCTGGCTGATCTTCAGAATC ATGCTTGGAGCGGGCCTCATCAAGGTCCGCGGGGACAAGTGCTGGCTGGATCTGACCTGCATGGACTTCC ACTATGAGACTCAGCCCGTGCCCAACCCGATAGCCTACTACCTGCACAGGTCACCATGGTGGTTCCACCG CTTTGAGACCCTCAGCAACCACTTCGTGGAGCTTCTTGTGCCTTTCTTCCTGTTCCTTGGACGGCGGATG CGCATTCTTCATGGAGTGCTGCAGATCCTTTTCCAGGTGATCCTCATCATCAGTGGGAACCTGAGCTTCC TGAACTGGCTCACCATCGTGCCCAGCCTTGCCTGCTTCGACGATGCAGCTCTGGGATTCCTGTTTCCCTC AGGACCTCAAGGCCTAAAGAAGCAAGTCCTGGAGATTCAGAGGGAAGATACACAAAGGGTCCAGCCCAAG CCAAGAGACAGAGGCTGCTTGGTGCGGCAAGTGGTCAACATCTCCCTGGGCATCCTGGTGGCCTGGCTCA GCGTACCCGTGGTTATCAATCTGCTAAGCTCCAGGCAGATCATGAACACCTCCTTCAACCCGCTCAGGAT CGTCAACACCTATGGGGCCTTTGGAAGTGTCACCAAAGAGCGGACTGAGGTGATCCTGCAGGGCACAGTG AGCCCCAACGCCAGCGCACCTGATGCAGTGTGGGAAGACTATGAGTTCAAGTGCAAACCGGGCGACCCCT GGAGGCAGCCATGCCTCATCTCGCCATACCACTACCGCCTGGACTGGCTGATGTGGTTCGCAGCTTTCCA GACCTATGAGCAGAACGAGTGGATCCTTCATCTGGCGGGTAAGCTCCTAGCTGGTGACTCTGAAGCCCTG GCCCTACTTGCTGTTAACCCCTTTGAGGGCAGGACTCCCCCCAGGTGGATCCGAGGAGAACACTACCGGT ACAAGTTCAGCCTCCCCGGGGGCCAGCACGCCACCCAGGGCAAGTGGTGGATCCGCAAGCGAATTGGTCC TTACTTCCCCCCACTCCGCCAGGAGGATCTGAAGGAATACTTTAAGACTCGGGAGTGGCCACTACCAGAA CCCCCATCTAGACACACACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208947 protein sequence
Red=Cloning site Green=Tags(s) MAAPEGSLRKRKVGGAEHSPASQPSLARDPADSPARLHTGTFWLTRIVLLRALAFIYFVAFLVAFNQNKA LIGDRGLLPCKLYLKNVQEYFQGSTGWAAWTYAPTIMWLLDWSDMNFNLDLIALLGLGISSFVLVTGCAN MILMTALWALYMSLVNVGQIWYSFGWESQLLETGFLGIFLSPLWTLSRLPKNTPTSQIVLWGFRWLIFRI MLGAGLIKVRGDKCWLDLTCMDFHYETQPVPNPIAYYLHRSPWWFHRFETLSNHFVELLVPFFLFLGRRM RILHGVLQILFQVILIISGNLSFLNWLTIVPSLACFDDAALGFLFPSGPQGLKKQVLEIQREDTQRVQPK PRDRGCLVRQVVNISLGILVAWLSVPVVINLLSSRQIMNTSFNPLRIVNTYGAFGSVTKERTEVILQGTV SPNASAPDAVWEDYEFKCKPGDPWRQPCLISPYHYRLDWLMWFAAFQTYEQNEWILHLAGKLLAGDSEAL ALLAVNPFEGRTPPRWIRGEHYRYKFSLPGGQHATQGKWWIRKRIGPYFPPLRQEDLKEYFKTREWPLPE PPSRHTR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029624 |
| ORF Size | 1704 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029624.2, NP_083900.2 |
| RefSeq Size | 1861 bp |
| RefSeq ORF | 1725 bp |
| Locus ID | 76483 |
| UniProt ID | Q3U3R4 |
| MW | 65.1 kDa |
| Gene Summary | Involved in the maturation of specific proteins in the endoplasmic reticulum. Required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway. Each LMF1 molecule chaperones 50 or more molecules of LPL.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203851 | Lmf1 (untagged) - Mouse lipase maturation factor 1 (Lmf1), (10ug) |
CNY 4,232.00 |
|
| MG208947 | Lmf1 (tGFP-tagged) - Mouse transmembrane protein 112 (Tmem112) |
CNY 5,040.00 |
|
| MR208947L3 | Lenti ORF clone of Lmf1 (Myc-DDK-tagged) - Mouse lipase maturation factor 1 (Lmf1) |
CNY 6,460.00 |
|
| MR208947L4 | Lenti ORF clone of Lmf1 (mGFP-tagged) - Mouse lipase maturation factor 1 (Lmf1) |
CNY 6,460.00 |
