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Rapgef5 (BC046627) Mouse Tagged ORF Clone
CAT＃: MR209090
- TrueORF®
Rapgef5 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175)
ORF Plasmid: tGFP
"BC046627" in other vectors (4)
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CNY 4,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mmr-gef, mKIAA0277, 4932413M22, GFR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209090 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCTCCCGCTTGCGGGTCTTTGACCCCCCTCTAGAGAGGAAAGATTCCGCGGCGCTCTCAGAGC GCCAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATCGACGAAGAAGATGAGGACGATGA ATGGAGCAGCCGCTCACAGTCTTCCACGGAGGATGACTCGGTGGACTCTCTGCTCTCTGACAGATATGTG GTGGTGTCTGGTACCCCGGAGAAGATCTTGGAGCACCTTTTGAATGACTTGCACCTGGCAGAGGTTCAGC ACAAAGAAACAGAAACACTCCTTGATGACTTCCTCCTCACGTACACCGTATTCATGACAACTGATGACTT GTGCCAGGCGCTGCTAAGACACTATTCTGCCAAGAAGTATCAAGGGGAAGAAGAAAACTCAGATGTCCCT TGTCGGAAGCGCAAGGTCTTACATCTGGTTTCCCAGTGGATTTCTCTGTACAAGGACTGGCTACATGAAG ATGAGCATTCAAAAATGTTTCTAAAGACCATATACAGGAACGTGCTGGATGATGTCTATGAGTACCCGAT TCTTGAGAAAGAGCTGAAAGAGTTTCAGAAGATACTTGGCGTGTACCGCCGTCACACTGTTGATGAATAC TCACCACAGAAGAAGAATAAAGCCCTTTTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCACAGAG GAACTGTGGCAGAAACAGAGGAAATTTTTTGCCACGTGTATATAACGGAGCACTCCTATATCAGTGTGAA GGCAAAAGTGTCGAGTACAGCCCAGGAGATCCTGAAGGTCGTGGCCGAGAAGCTGCAGCGTGCCAAAGAG GATCTGGCTCTGGTAGCCATTACATTCTCTGGAGAAAAGCATGAATTTCAGCCAAATGACTTGGCCATTT CCAAATCCCTGGAGGCATCTGGTCGGATATATGTCTACCGAAAAGATCTGGCTGACACTTTGAATCCTTT GGCAGAAAATGAGGAATCCCAGCAAAGGTCAATGAGGATTTTGGGGATGAACACGTGGGACCTTGCTCTG GAATTAATGAGTTTTGATTGGAGTCTGTTCAATTCAATCCACGAGCAAGAGCTGATCTACTTCACATTCA GCAGACAGGGGAACGGAGAGAATACTGTGAACCTCAGCCTTCTGCTCCAGAGATGTAACGAGGTCCAGCT CTGGGTAGCCACAGAGATTCTGCTCTGCAGCCAGCTAGGCAAGCGCGTGCAGCTGGTGAAAAAATTCATC AAGATTGCAGCTCACTGCAAAGCCCAACAGAACCTAAACTCTTTCTTTGCCATCGTGATGGGTCTCAACA CCGCCTCTGTCAGCCGGCTATCACAGACCTGGGAGAAAATCCCTGGGAAGTTTAAGAAGCTTTTCTCTGA ACTTGAGAGTTTAACGGATCCTTCCCTAAACCACAAAGCCTACAGGGATGCGTTCAAAAAGATGAAGCCA CCAAAAATCCCTTTCATGCCCTTACTGCTTAAAGATGTAACGTTTATTCATGAAGGAAATAAAACATTCT TGGATAATCTCGTCAATTTTGAAAAGCTGCACATGATTGCAGACACTGTCCGAACCCTGAGACACTGCAG GACTAACCAGTTTGGAAGTGACGTGTCTCCAAAGGAGCAGCAAGAGCTGAAGTCCTATGTCAACCACCTG TATGTCATCGACAGCCAGCAAGCCCTGTTCGAGCTCTCTCACAGGCTGGAGCCTCGAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209090 protein sequence
Red=Cloning site Green=Tags(s) MGSSRLRVFDPPLERKDSAALSERQLPLPTFDVPYFKYIDEEDEDDEWSSRSQSSTEDDSVDSLLSDRYV VVSGTPEKILEHLLNDLHLAEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQGEEENSDVP CRKRKVLHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKILGVYRRHTVDEY SPQKKNKALFHQFSLKENWLQHRGTVAETEEIFCHVYITEHSYISVKAKVSSTAQEILKVVAEKLQRAKE DLALVAITFSGEKHEFQPNDLAISKSLEASGRIYVYRKDLADTLNPLAENEESQQRSMRILGMNTWDLAL ELMSFDWSLFNSIHEQELIYFTFSRQGNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFI KIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKP PKIPFMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGSDVSPKEQQELKSYVNHL YVIDSQQALFELSHRLEPRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC046627 |
| ORF Size | 1740 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC046627, AAH46627 |
| RefSeq Size | 2954 bp |
| RefSeq ORF | 1742 bp |
| Locus ID | 217944 |
| MW | 67.5 kDa |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218582 | Rapgef5 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175), (10ug) |
CNY 6,940.00 |
|
| MG209090 | Rapgef5 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 3,710.00 |
|
| MR209090L3 | Lenti ORF clone of Rapgef5 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 5,230.00 |
|
| MR209090L4 | Lenti ORF clone of Rapgef5 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 5,230.00 |
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