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F12 (NM_021489) Mouse Tagged ORF Clone
CAT＃: MR209276
- TrueORF®
F12 (Myc-DDK-tagged) - Mouse coagulation factor XII (Hageman factor) (F12)
ORF Plasmid: tGFP
"NM_021489" in other vectors (4)
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CNY 4,456.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FX; FXII; HAF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209276 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCTCTGTTGTTCCTGGGGTCTCTGCTGATGAGTCTGGATCTGACACTTTCGGCTCCACCATGGA AAGACTCCAAGAAATTTAAGGACGCACCTGATGGGCCCACAGTGGTTCTCACTGTGGATGGGAGGCTCTG CCATTTTCCCTTTCAGTACCACCGTCAGCTACACCACAAATGCATCCACAAAAGGCGGCCAGGCTCCCGC CCCTGGTGTGCTACCACCCCCAACTTTGATGAAGATCAGCAATGGGGATACTGCTTGGAGCCCAAGAAAG TGAAAGACCATTGCAGCAAACACAACCCGTGCCACAAAGGAGGGACATGTATCAACACCCCCAATGGGCC ACACTGTCTCTGCCCTGAACACCTCACTGGGAAACATTGCCAGAAAGAGAAATGCTTTGAGCCTCAGCTT CTCAAGTTCTTCCACGAGAATGAGCTATGGTTTAGAACGGGGCCAGGAGGTGTGGCCAGGTGCGAGTGCA AAGGTTCTGAGGCTCACTGCAAGCCGGTGGCCAGCCAGGCCTGCAGCATCAATCCGTGCCTTAATGGGGG CAGCTGCCTCCTCGTGGAGGACCACCCACTGTGCCGTTGCCCTACAGGCTACACTGGATATTTTTGCGAC TTGGACCTTTGGGCGACCTGCTATGAAGGCAGGGGGCTCAGCTACCGGGGCCAGGCTGGAACTACGCAAT CGGGTGCGCCATGTCAGCGGTGGACCGTGGAGGCCACCTACCGGAACATGACTGAGAAGCAAGCGCTAAG CTGGGGCCTGGGCCACCACGCATTTTGCCGGAACCCAGATAATGACACACGTCCATGGTGCTTCGTCTGG AGTGGCGACAGGCTGAGCTGGGACTATTGCGGCCTGGAGCAGTGCCAGACGCCAACGTTTGCACCTCTAG TTGTCCCTGAGAGTCAGGAGGAGTCCCCGTCCCAGGCACCATCTCTGTCCCATGCACCAAATGACTCGAC CGATCATCAGACTTCTCTGTCCAAGACCAACACGATGGGCTGCGGACAGAGGTTCCGCAAGGGACTGTCC TCGTTCATGCGCGTGGTGGGCGGACTAGTGGCTCTGCCTGGGTCGCACCCCTACATCGCTGCACTGTACT GGGGTAACAACTTCTGCGCGGGCAGTCTCATCGCCCCCTGTTGGGTGCTGACCGCGGCTCACTGCCTGCA GAATCGGCCAGCGCCCGAGGAACTGACAGTGGTACTTGGTCAAGATCGCCACAACCAGAGCTGCGAGTGG TGCCAGACTCTGGCTGTGCGCTCCTACCGCCTTCACGAGGGCTTCTCCTCCATCACCTACCAGCACGACT TGGCTCTGCTGCGCCTGCAGGAAAGCAAAACCAACAGTTGCGCGATCCTGTCACCTCACGTTCAGCCTGT GTGTCTACCCAGCGGCGCGGCCCCACCCTCTGAGACAGTGCTCTGCGAGGTGGCCGGCTGGGGTCACCAG TTCGAGGGGGCTGAAGAATACTCCACCTTCCTGCAGGAGGCACAGGTTCCCTTTATCGCCCTGGATCGCT GCTCCAACTCTAACGTGCACGGAGACGCCATTCTCCCTGGGATGCTTTGCGCTGGCTTCTTGGAGGGAGG CACCGATGCCTGCCAGGGTGACTCCGGGGGCCCTCTGGTGTGTGAGGAAGGAACTGCAGAACATCAGCTC ACCCTGCGCGGAGTCATCAGCTGGGGCTCCGGCTGTGGTGACCGCAACAAGCCCGGAGTCTACACAGACG TGGCCAACTACCTGGCTTGGATCCAGAAGCATATTGCTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209276 protein sequence
Red=Cloning site Green=Tags(s) MTALLFLGSLLMSLDLTLSAPPWKDSKKFKDAPDGPTVVLTVDGRLCHFPFQYHRQLHHKCIHKRRPGSR PWCATTPNFDEDQQWGYCLEPKKVKDHCSKHNPCHKGGTCINTPNGPHCLCPEHLTGKHCQKEKCFEPQL LKFFHENELWFRTGPGGVARCECKGSEAHCKPVASQACSINPCLNGGSCLLVEDHPLCRCPTGYTGYFCD LDLWATCYEGRGLSYRGQAGTTQSGAPCQRWTVEATYRNMTEKQALSWGLGHHAFCRNPDNDTRPWCFVW SGDRLSWDYCGLEQCQTPTFAPLVVPESQEESPSQAPSLSHAPNDSTDHQTSLSKTNTMGCGQRFRKGLS SFMRVVGGLVALPGSHPYIAALYWGNNFCAGSLIAPCWVLTAAHCLQNRPAPEELTVVLGQDRHNQSCEW CQTLAVRSYRLHEGFSSITYQHDLALLRLQESKTNSCAILSPHVQPVCLPSGAAPPSETVLCEVAGWGHQ FEGAEEYSTFLQEAQVPFIALDRCSNSNVHGDAILPGMLCAGFLEGGTDACQGDSGGPLVCEEGTAEHQL TLRGVISWGSGCGDRNKPGVYTDVANYLAWIQKHIAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021489 |
| ORF Size | 1794 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021489.3 |
| RefSeq Size | 1961 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 58992 |
| UniProt ID | Q80YC5 |
| MW | 65.7 kDa |
| Gene Summary | This gene encodes a glycoprotein coagulation factor that plays an important role in the intrinsic pathway of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that is autoactivated upon contact with negatively charged surfaces or misfolded protein aggregates. Mice lacking the encoded protein have a severe defect in forming stable fibrin clots. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203280 | F12 (untagged) - Mouse coagulation factor XII (Hageman factor) (F12), (10ug) |
CNY 4,456.00 |
|
| MG209276 | F12 (tGFP-tagged) - Mouse coagulation factor XII (Hageman factor) (F12) |
CNY 5,800.00 |
|
| MR209276L3 | Lenti ORF clone of F12 (Myc-DDK-tagged) - Mouse coagulation factor XII (Hageman factor) (F12) |
CNY 7,130.00 |
|
| MR209276L4 | Lenti ORF clone of F12 (mGFP-tagged) - Mouse coagulation factor XII (Hageman factor) (F12) |
CNY 7,130.00 |
