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Papss1 (BC066055) Mouse Tagged ORF Clone
CAT＃: MR209336
- TrueORF®
Papss1 (Myc-DDK-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756)
ORF Plasmid: tGFP
"BC066055" in other vectors (4)
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CNY 4,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI325286; Asapk; SK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209336 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAGCAACCAACGTCACCTATCAAGCCCACCATGTCAGCAGGAATAAGAGAGGGCAGGTGGTGG GGACCAGAGGTGGCTTCCGTGGTTGCACAGTTTGGCTGACAGGATTGTCCGGAGCAGGGAAGACGACCGT GAGCATGGCTCTGGAGGAGTACCTGGTGTGCCACGGCATTCCGTGCTACACTTTGGATGGTGACAACATC CGCCAAGGACTCAATAAGAACCTCGGCTTCAGTCCTGAGGACAGAGAAGAGAACGTTCGCCGCATAGCTG AGGTGGCGAAGCTGTTTGCAGATGCTGGCTTAGTGTGTATCACCAGCTTTATATCGCCTTACACACAGGA TCGCAACAACGCAAGGCAGATTCATGAGGGTGCAAGCTTGCCCTTCTTTGAAGTTTTTGTCGATGCTCCT CTGCATGTCTGTGAGCAGAGGGATGTCAAAGGCCTCTACAAGAAGGCGCGGGCAGGGGAGATAAAAGGCT TCACTGGCATCGATTCTGAGTATGAGAAACCCGAGGCCCCGGAGCTGGTGCTGAAAACGGATTCCTGTGA CGTCAACGACTGCGTCCAGCAGGTTGTGGAGCTTCTTCAGGAACGGGACATCGTCCCTGTGGATGCTTCC TATGAAGTGAAAGAGCTATATGTGCCAGAGAATAAACTTCACTTGGCCAAAACTGATGCAGAAGCCTTAC CAGCCCTGAAAATCAATAAAGTGGATATGCAGTGGGTGCAGGTTTTGGCAGAAGGTTGGGCGACTCCTCT GAACGGCTTCATGAGAGAGAGGGAGTACTTGCAGTGCCTCCATTTCGATTGTCTTCTGGATGGAGGCGTC ATCAACTTATCGGTGCCTATAGTTCTGACAGCTACGCACGAGGATAAGGAGAGGCTGGACGGCTGCACCG CGTTCGCTCTGGTGTATGAGGGCCGCCGCGTGGCCATCCTTCGGAATCCTGAATTTTTTGAGCACCGGAA AGAGGAGCGGTGTGCCAGACAGTGGGGAACAACATGCAAGAACCACCCCTACATCAAGATGGTTCTGGAA CAAGGGGATTGGCTGATTGGAGGAGATCTTCAAGTCCTGGACCGGATTTACTGGAATGATGGTCTTGATC AGTACCGCCTTACCCCGACGGAGCTCAAGCAGAAGTTTAAAGATATGAACGCTGATGCTGTCTTTGCATT TCAGTTGCGCAACCCAGTGCACAACGGGCACGCTCTGTTAATGCAGGATACCCACAAGCAGCTTCTGGAG AGGGGCTACCGGCGCCCTGTCCTGCTCCTCCATCCTCTTGGTGGCTGGACGAAGGATGACGATGTCCCTC TGATGTGGCGTATGAAGCAGCACGCTGCAGTGCTGGAGGAGGGCATCCTGGATCCTGAAACGACAGTGGT GGCCATTTTCCCGTCTCCTATGATGTATGCTGGGCCAACCGAGGTCCAGTGGCACTGCAGGGCGCGGATG GTGGCCGGAGCCAATTTTTATATTGTTGGACGAGACCCTGCTGGCATGCCTCATCCGGAGACAGGGAAGG ACCTCTATGAGCCAACACATGGTGCCAAAGTGCTGACGATGGCCCCAGGCCTGATTACCTTGGAAATCGT TCCCTTCCGAGTTGCAGCTTACAACAAGAAAAAGAAGCGGATGGACTACTATGACTCTGAGCACCACGAA GACTTCGAGTTTATTTCAGGAACGAGGATGCGCAAGCTGGCACGCGAAGGCCAGAAACCTCCAGAGGGCT TCATGGCCCCCAAGGCCTGGACTGTGCTGGTAGAGTACTACAAGTCCTTAGAGAAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209336 protein sequence
Red=Cloning site Green=Tags(s) MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNI RQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAP LHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDAS YEVKELYVPENKLHLAKTDAEALPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGV INLSVPIVLTATHEDKERLDGCTAFALVYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVLE QGDWLIGGDLQVLDRIYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLE RGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGILDPETTVVAIFPSPMMYAGPTEVQWHCRARM VAGANFYIVGRDPAGMPHPETGKDLYEPTHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHE DFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLVEYYKSLEKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC066055 |
| ORF Size | 1809 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066055, AAH66055 |
| RefSeq Size | 2673 bp |
| RefSeq ORF | 1811 bp |
| Locus ID | 23971 |
| MW | 68.5 kDa |
| Gene Summary | Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:7493984). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218279 | Papss1 (untagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756), (10ug) |
CNY 6,940.00 |
|
| MG209336 | Papss1 (tGFP-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 3,800.00 |
|
| MR209336L3 | Lenti ORF clone of Papss1 (Myc-DDK-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 5,320.00 |
|
| MR209336L4 | Lenti ORF clone of Papss1 (mGFP-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 5,320.00 |
