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Cdc16 (NM_027276) Mouse Tagged ORF Clone
CAT＃: MR209493
- TrueORF®
Cdc16 (Myc-DDK-tagged) - Mouse CDC16 cell division cycle 16 homolog (S. cerevisiae) (Cdc16)
ORF Plasmid: tGFP
"NM_027276" in other vectors (4)
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CNY 4,616.00
CNY 5,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700071J12Rik; 2810431D22Rik; APC6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209493 representing NM_027276
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTGGAGCCGCTGCGCAAACGCGTCCGGCAGTACCTGGACCAGCAACAGTATCAAAGTGCTCTGT TTTGGGCAGACAAGGTAGCTTCACTCTCTCATGAGGAGCCCCAGGATGTTTATTGGTTGGCTCAGTGCCT TTACCTCACAGCGCAGTACCACCGAGCAGCCCATGCGCTCCGGTCACGCAAGCTGGACAAATTGTATGAA GCTTGCCGATACCTTGCAGCTAGATGCCACTATGCTGCAAAAGAGCACCAGCAGGCTCTGGACATCCTTG ACATGGAGGAGCCAATTAACAGAAGGCTCTTTGAGAAATACCTGAAGGATGACAATGGCTCGAGAGACCC TTCCAGTGACTGGGAGATGTCACAGTCTTCCATAAAGAGCTCGATTTGTCTCCTGAGAGGGAAGATCTAT GATGCTTTAGATAATCGCACCCTGGCTACCTACAGTTACAAAGAGGCTCTGAAACTGGATGTCTACTGCT TTGAAGCCTTTGACCTTTTGACATCACACCACATGCTGACAGCACAGGAAGAAAAAGAACTTCTGGACTC CCTGCCTCTTAACAAGCTCTGTGCTGAAGAACAAGAGTTGCTGCGATTTGTATTTGAGAACAAATTAAAG AAGTATAATAAGCCCAGTGAAACGGTCATTCCCGAGTCAGTGGACGGCTTGCAGGAGAATCTGGACGTGG TAGTGTCTTTAGCTGAGAGACACTATTACAACTGTGATTTTAAGATGTGCTACAAGCTTACTTCTACAGT GATGGAGAAAGATCCTTTCCATGCAAACTGTTTACCTGTGCACATAGGAACTCTTGTGGAGCTGAATAAA GCGAATGAACTTTTCTATCTTTCTCACAAACTGGTGGATTTATATCCGAGTAATCCTGTGTCTTGGTTTG CGGTGGGATGTTACTATCTCATGGTTGGTCATAAAAATGAGCATGCCAGAAGATATCTCAGCAAAGCTAC CACGTTGGAGAAGACCTACGGTCCTGCCTGGATAGCCTACGGGCATTCCTTCGCTGTGGAGAGTGAGCAC GACCAAGCAATGGCTGCTTACTTCACAGCAGCACAGCTGATGAAGGGGTGTCACTTGCCAATGCTATATA TTGGATTAGAATATGGTCTGACCAATAACTCGAAACTGGCTGAGCGGTTCTTTGGCCAAGCTCTGAGCAT CGCCCCAGAAGACCCATTTGTCATCCATGAGGTTGGAGTGGTTGCCTTTCAGAATGGAGAATGGAAAACA GCAGAAAAATGGTTTCTCGATGCTTTGGAAAAAATTAAAGCAATTGGAAATGAGGTCACAGTTGACAAAT GGGAACCTTTGTTGAACAACTTGGGGCATGTCTGCAGAAAACTTAAGAAATATGCTGAGGCTTTGGATTA CCACAGGCAGGCACTGGTGCTAATCCCTCAGAACGCATCCACCTACTCTGCTATAGGGTACATCCACAGT CTGATGGGCAACTTTGAAAACGCTGTGGACTATTTCCACACAGCTCTTGGTCTCAGGCGAGACGACACAT TTTCTGTTACAATGCTTGGTCACTGCATTGAAATGTACATCGGGGATTCGGAAGCTTATATCGGAGCAGA CATTAAAGACAAATTAAAGTGCTATGACTTCGATGTGCATACAATGAAAACACTAAAAAACATCATTTCG CCTCCATGGGATTTCAGGGACTTTGAAGTAGAAAAGCAGAACACAGAGGAAGCGGGACTTGCACCTCTGC AAAACTCCACTAAAGCTCCAGAGTCCAGACCTAACTTGGAAGAGACTTTTGAAATTGAAATGAACGAAAG TGACATGATGCTAGAGACGTCTATGTCAGACCACAGCACA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR209493 representing NM_027276
Red=Cloning site Green=Tags(s) MNLEPLRKRVRQYLDQQQYQSALFWADKVASLSHEEPQDVYWLAQCLYLTAQYHRAAHALRSRKLDKLYE ACRYLAARCHYAAKEHQQALDILDMEEPINRRLFEKYLKDDNGSRDPSSDWEMSQSSIKSSICLLRGKIY DALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLDSLPLNKLCAEEQELLRFVFENKLK KYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSTVMEKDPFHANCLPVHIGTLVELNK ANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEH DQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFGQALSIAPEDPFVIHEVGVVAFQNGEWKT AEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHS LMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIIS PPWDFRDFEVEKQNTEEAGLAPLQNSTKAPESRPNLEETFEIEMNESDMMLETSMSDHST SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027276 |
| ORF Size | 1860 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027276.3 |
| RefSeq Size | 2253 bp |
| RefSeq ORF | 1863 bp |
| Locus ID | 69957 |
| UniProt ID | Q8R349 |
| MW | 71.9 kDa |
| Gene Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202128 | Cdc16 (untagged) - Mouse CDC16 cell division cycle 16 homolog (S. cerevisiae) (Cdc16), (10ug) |
CNY 4,624.00 |
|
| MG209493 | Cdc16 (tGFP-tagged) - Mouse CDC16 cell division cycle 16 homolog (S. cerevisiae) (Cdc16) |
CNY 6,080.00 |
|
| MR209493L3 | Lenti ORF clone of Cdc16 (Myc-DDK-tagged) - Mouse CDC16 cell division cycle 16 homolog (S. cerevisiae) (Cdc16) |
CNY 7,410.00 |
|
| MR209493L4 | Lenti ORF clone of Cdc16 (mGFP-tagged) - Mouse CDC16 cell division cycle 16 homolog (S. cerevisiae) (Cdc16) |
CNY 7,410.00 |
