Amot (BC057638) Mouse Tagged ORF Clone

CAT#: MR209494

  • TrueORF®

Amot (Myc-DDK-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191)

ORF Plasmid: DDK tGFP



  "BC057638" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,180.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CAG-2, mKIAA1071
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209494 representing BC057638
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACACCGAGGTCCCCCGCCAGAATACCCTTTCAAGGGCGTGCCATCCCAGTCTGTAGTGTGCAAGT
CCCAAGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGAT
GAGGTATCAGCACCCCCCTGAGTATGGGGCAGCCAGGGCAACACAGGACATCTCCTCGCTGTCTTTGTCA
GCCAGGAACTCTCAACCTCATAGCCCTACTTCTTCCCTGACAGCTGGGGCTTCCAGCTTGCCCTTGCTGC
AGTCTCCGCCGTCCACTAGATTGCCTCCTGGTCAACACCTGGTCCCGAACCAAGGTGACCACTCTGCTCA
CCTGTCAAGGCATCAGCAGCATCTTCTTTCGAGTCAGAGTCATCAGGGGGACCACTACCGTCACGCCCAG
GCCAGCCTGACTTCAGCGCAGCAGCAGCCAGGAGAAGCCTACTCAGCTATGCCTCGGGCTCAGCAGTCTG
CTTCTTATCAGCCCATGCCAGCTGACCCTTTTGCTATCGTCTCTAGAGCTCAGCAGATGGTTGAGATCCT
GTCTGATGAGAATCGCAACTTGCGGCAGGAGTTGGATGGATGCTATGAGAAGGTGGCGAGACTGCAGAAG
GTGGAGACAGAAATCCAACGGGTCTCAGAGGCTTATGAGAACTTGGTGAAGTCATCTTCCAAAAGAGAGG
CTCTGGAGAAAGCCATGAGGAACAAGCTGGAGGGCGAGATTAGAAGGATGCATGACTTCAACAGAGATCT
GAGAGACCGTCTAGAGACTGCCAACAAGCAGCTGGCAGAGAAGGAGTACGAGGGGTCCGAGGACACCAGG
AAGACCATCTCGCAGCTCTTTGCCAAACATAAAGAAAACCAGCGGGAGAAGGAGAAGCTGGAAGCTGAGC
TGGCTACTGCCCGTTCTACCAATGAAGACCAAAGGCGACACATTGAAATCCGAGACCAGGCATTGAGCAA
TGCCCAGGCGAAGGTGGTGAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTAGATAAGGTGGAG
AAGATGCAGCAGGCCCTTGTGCAGCTCCAGGCCGCCTGTGAGAAGCGTGAGCAGCTGGAACACCGGCTCC
GGACACGTTTGGAGAGGGAACTGGAATCCCTTAGAATCCAGCAGCGCCAGGGCAACTCCCAGCCCACTAA
TGCTTCAGAATACAATGCCGCTGCACTGATGGAGCTCCTCCGGGAGAAGGAAGAGAGAATTCTGGCCCTG
GAAGCAGATATGACCAAGTGGGAGCAAAAGTATTTGGAAGAGAATGTGATGAGACACTTTGCTCTGGATG
CTGCTGCAACTGTGGCTGCACAGAGAGACACAACAGTCATTAGCCACTCTCCTAACACCAGCTATGACAC
AGCCTTAGAGGCCCGCATCCAGAAGGAAGAGGAAGAAATCCTGATGGCCAACAAGCGTTGCTTGGACATG
GAAGGAAGGATTAAGACCCTCCATGCCCAGATTATTGAAAAGGATGCCATGATCAAGGTGCTGCAGCAGC
GCTCCAGAAAGGAGCCGAGTAAAACAGAGCAACTCTCATCCATGCGGCCAGCAAAGTCTCTGATGTCCAT
TTCCAATGCTGGATCAGGCCTCCTTGCATACTCCTCTACCTTGACTGGTGCGCCCATTATGGAGGAGAAG
CGTGATGACAAGAGCTGGAAGGGGAGCCTAGGTAAGGAAAAGGAAAGCAACAGAAGCAAAGGCACAGTGA
CAGACTTAGAAAGTGTCCTGACTCTGTTACACACAGCAAGGAAAAGGGACAATGGTCCAGGTTCTAGAGA
GGAGAATTTGGAGTCCCCCCTCTCAATGGAATTAGATTTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209494 representing BC057638
Red=Cloning site Green=Tags(s)

MEHRGPPPEYPFKGVPSQSVVCKSQEPGHFYSEHRLNQPGRTEGQLMRYQHPPEYGAARATQDISSLSLS
ARNSQPHSPTSSLTAGASSLPLLQSPPSTRLPPGQHLVPNQGDHSAHLSRHQQHLLSSQSHQGDHYRHAQ
ASLTSAQQQPGEAYSAMPRAQQSASYQPMPADPFAIVSRAQQMVEILSDENRNLRQELDGCYEKVARLQK
VETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRDRLETANKQLAEKEYEGSEDTR
KTISQLFAKHKENQREKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVE
KMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNSQPTNASEYNAAALMELLREKEERILAL
EADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDM
EGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSSMRPAKSLMSISNAGSGLLAYSSTLTGAPIMEEK
RDDKSWKGSLGKEKESNRSKGTVTDLESVLTLLHTARKRDNGPGSREENLESPLSMELDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057638
ORF Size 1860 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057638.1
RefSeq Size 2405 bp
RefSeq ORF 1862 bp
Locus ID 27494
MW 88.1 kDa
Gene Summary Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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