F11 (NM_028066) Mouse Tagged ORF Clone

CAT#: MR209529

  • TrueORF®

F11 (Myc-DDK-tagged) - Mouse coagulation factor XI (F11)

ORF Plasmid: DDK tGFP



  "NM_028066" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1600027G01Rik; AI503996; Cf11; FX; FXI; PTA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209529 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTCATTACATCAGGTGTTATATTTTATCTTTTTTGCCTCAGTTTCTAGTGAATGCGTTACTAAGG
TCTTCAAAGACATCAGCTTTCAAGGAGGTGACCTGAGTACTGTTTTCACACCGAGCGCCACATACTGCCG
CTTGGTCTGCACTCACCACCCACGGTGCTTGCTCTTCACGTTCATGGCTGAGTCATCTTCGGATGATCCT
ACCAAATGGTTTGCCTGCATCCTGAAGGACAGCGTCACAGAAATATTGCCAATGGTAAACATGACAGGCG
CGATCTCTGGATATTCCTTCAAGCAATGCCCTCAGCAATTAAGTACTTGCAGCAAAGATGTGTACGTGAA
CCTAGACATGAAGGGCATGAACTATAACAGCTCTGTCGTGAAGAATGCTCGAGAATGCCAGGAGAGATGC
ACAGACGATGCCCACTGCCAGTTTTTCACATACGCAACAGGGTATTTTCCCAGTGTGGACCATCGTAAAA
TGTGTCTTTTGAAGTACACCCGAACGGGGACGCCAACCACAATAACGAAGCTCAATGGCGTGGTATCTGG
ATTTTCACTGAAGTCCTGTGGACTTTCAAACTTGGCTTGTATCAGGGACATTTTCCCTAACACGGTGCTG
GCAGACCTTAACATTGACAGCGTGGTGGCCCCAGATGCTTTTGTCTGTCGTCGCATCTGCACGCATCACC
CCACTTGTTTGTTCTTCACATTCTTTTCCCAAGCATGGCCGAAAGAATCTCAGAGACATCTTTGTCTCCT
TAAAACCTCTGAAAGTGGATTACCAAGCACACGCATTACAAAGAGCCACGCCCTTTCGGGCTTCAGTCTC
CAGCACTGCAGGCACAGTGTCCCAGTATTCTGCCATCCGTCCTTTTACAACGACACTGATTTCTTGGGAG
AAGAGCTGGACATCGTCGATGTGAAAGGCCAAGAAACCTGTCAGAAAACGTGTACCAATAACGCCCGCTG
CCAGTTCTTTACCTACTATCCATCGCACAGACTGTGCAATGAGAGGAACCGCAGGGGCAGATGTTACCTA
AAGCTTTCCTCCAATGGATCTCCAACGAGAATACTTCATGGGAGGGGAGGCATCTCTGGATACTCACTGA
GGCTGTGCAAAATGGATAATGTGTGCACAACTAAAATCAACCCCAGAGTGGTAGGAGGAGCTGCGTCTGT
TCACGGTGAGTGGCCATGGCAGGTGACTCTGCACATCAGCCAGGGACACCTGTGTGGAGGCTCCATCATT
GGAAACCAATGGATACTGACAGCAGCTCATTGTTTCTCTGGGATAGAGACACCTAAAAAGCTGCGTGTCT
ACGGTGGCATTGTAAATCAATCAGAAATAAATGAAGGGACTGCTTTCTTCAGGGTTCAAGAAATGATAAT
TCATGATCAGTATACGACAGCAGAAAGTGGGTATGATATTGCCCTGTTAAAACTGGAATCAGCCATGAAT
TACACAGATTTTCAGCGGCCAATATGCCTGCCTTCCAAAGGAGATAGAAACGCAGTGCACACAGAATGCT
GGGTGACTGGATGGGGGTACACAGCACTAAGAGGTGAAGTACAAAGTACTCTTCAGAAAGCCAAGGTTCC
ATTGGTGTCAAATGAAGAATGTCAGACAAGATACAGAAGACACAAAATAACCAATAAGATGATCTGTGCA
GGCTACAAAGAAGGAGGGAAGGATACGTGCAAGGGAGATTCTGGAGGGCCCCTGTCCTGCAAATACAATG
GGGTCTGGCACTTGGTGGGCATCACAAGCTGGGGTGAAGGCTGTGGTCAGAAGGAGAGACCGGGGGTCTA
CACGAACGTGGCCAAGTACGTGGACTGGATTCTGGAGAAAACTCAAACAGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209529 protein sequence
Red=Cloning site Green=Tags(s)

MTSLHQVLYFIFFASVSSECVTKVFKDISFQGGDLSTVFTPSATYCRLVCTHHPRCLLFTFMAESSSDDP
TKWFACILKDSVTEILPMVNMTGAISGYSFKQCPQQLSTCSKDVYVNLDMKGMNYNSSVVKNARECQERC
TDDAHCQFFTYATGYFPSVDHRKMCLLKYTRTGTPTTITKLNGVVSGFSLKSCGLSNLACIRDIFPNTVL
ADLNIDSVVAPDAFVCRRICTHHPTCLFFTFFSQAWPKESQRHLCLLKTSESGLPSTRITKSHALSGFSL
QHCRHSVPVFCHPSFYNDTDFLGEELDIVDVKGQETCQKTCTNNARCQFFTYYPSHRLCNERNRRGRCYL
KLSSNGSPTRILHGRGGISGYSLRLCKMDNVCTTKINPRVVGGAASVHGEWPWQVTLHISQGHLCGGSII
GNQWILTAAHCFSGIETPKKLRVYGGIVNQSEINEGTAFFRVQEMIIHDQYTTAESGYDIALLKLESAMN
YTDFQRPICLPSKGDRNAVHTECWVTGWGYTALRGEVQSTLQKAKVPLVSNEECQTRYRRHKITNKMICA
GYKEGGKDTCKGDSGGPLSCKYNGVWHLVGITSWGEGCGQKERPGVYTNVAKYVDWILEKTQTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028066
ORF Size 1875 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028066.2
RefSeq Size 2246 bp
RefSeq ORF 1875 bp
Locus ID 109821
UniProt ID Q91Y47
MW 69.8 kDa
Gene Summary This gene encodes a glycoprotein coagulation factor that plays an important role in intrinsic pathway of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that can be activated by coagulation factor XIIa, thrombin or factor XIa to generate active factor XIa protease. Mice lacking the encoded protein display a survival advantage during peritoneal sepsis and resist inflammation and bacterial accumulation upon infection with Listeria. [provided by RefSeq, Apr 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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