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Jph4 (BC052049) Mouse Tagged ORF Clone
CAT＃: MR209581
- TrueORF®
Jph4 (Myc-DDK-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347)
ORF Plasmid: tGFP
"BC052049" in other vectors (4)
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CNY 4,680.00
CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JP-4, JPHL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209581 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCCGGGGGCAAGTTCGACTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGG CACATGGCTACGGCGTGTGCACCGGGCCCGGCGCCCAGGGAGAGTACAGCGGCTGCTGGGCTCACGGCTT CGAGTCGCTGGGTGTCTTCACGGGGCCCGGCGGACACAGTTACCAGGGCCACTGGCAGCAGGGCAAGCGC GAAGGGCTGGGCGTGGAGCGCAAGAGCCGCTGGACCTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGC GCAGCGGCGTATGGGAGAGCGTGTCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGCTTCCAGGACGG CTACGGCACCGAGACCTACTCGGATGGAGGCACTTACCAAGGCCAGTGGCAAGCCGGGAAACGCCACGGC TACGGGGTGCGCCAAAGTGTACCCTACCACCAGGCGGCGCTTCTGCGCTCACCCCGCCGCACCTCCCTGG ACTCCGGCCACAGCGACCCCCCGACGCCGCCTCCACCCCTACCCCTACCAGGAGATGAAGGAGGCAGCCC AGCCTCCGGCTCCCGAGGCGGCTTCGTGCTGGCGGGGCCAGGGGACGCCGACGGGGCGTCTTCCCGTAAG CGCACCCCAGCAGCAGGTGGATTCTTCCGCCGTTCACTGCTGCTCAGCGGGCTCCGGGCTGGTGGGCGCC GCAGCTCCCTGGGCAGCAAGAGGGGATCTCTACGCAGCGAGGTGAGCAGCGAAGTGGGCAGTACAGGACC CCCGGGCTCCGAGGCCAGCGGGCCCCCAATCCCAGCTCCACCTGCCCTCATCGAGGGCTCAGCCACTGAG GTGTACGCCGGTGAGTGGCGTGCGGACCGGCGCAGTGGCTATGGAGTGAGCCAGAGATCCAACGGGCTGC GCTACGAAGGCGAGTGGCTGGGCAACCGACGCCACGGATACGGGCGCACCACCCGGCCCGATGGCTCCCG TGAGGAGGGCAAGTACAAGCGTAACCGGCTGGTACACGGGGGACGTGTCCGCAGCCTCCTGCCTCTGGCC CTTAGACGGGGCAAAGTCAAGGAGAAAGTGGACAGGGCTGTCGAAGGTGCCCGTCGAGCTGTGAGTGCTG CCCGCCAGCGCCAGGAAATTGCGGCTGCCAGGGCAGCAGACGCCCTCCTAAAAGCAGTGGCAGCCAGCAG CGTCGCGGAGAAGGCTGTGGAGGCTGCGCGGATGGCCAAGCTGATAGCCCAGGATCTACAACCCATGTTA GAGGCCCCAGGCCGCAGACCCAGACAGGACTCAGGAGGTTCTGACACAGAGCCTTTGGATGAGGACAGCC CTGGGGTATACGAGAATGGACTGACCCCCTCAGAGGGCTCTCCGGAACTACCCAGCAGCCCCGCCTCCTC CCACCAACCTTGGCGAGCCCCTGCCTGCCGGAGTCCACTGCCTCCTGGAGGGAACTGGGGCCCCTTCTCC AGCCCTAAAGCTTGGCCCGAGGAGTGGGGGGGTCCTGGTGAGCAGGCAGAGGAACTAGCTGGCTATGAGG CTGAGGATGAGGCTGGGGTGCAGGGTCCAGGACCAAGAGATGGTTCCCCACTTCTCGGAGGCTGCAGTGA CAGTTCAGGAAGTCTTCGAGAGGAGGAGGGGGAAGACGAAGAGTCCTTGCCCCAACTGAGGGCCCCAGGA GGCTCAGAGTCTGAGCCTGTCACCACGCCAGTTTTGAGGGGCCTGTCTTCAAGGGGTCCTGATGCTGGGT GCCTGACAGAAGAGGTTGAGGAGCCTGCTCCCACCGAGAGGCCTGCCCAGCCGGGAGCTGCCAACCCCCT GGTGGTGGGAGCCGTGGCCTTGCTGGACCTCAGCCTGGCCTTCCTGTTCTCCCAGCTCCTCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209581 protein sequence
Red=Cloning site Green=Tags(s) MSPGGKFDFDDGGCYVGGWEAGRAHGYGVCTGPGAQGEYSGCWAHGFESLGVFTGPGGHSYQGHWQQGKR EGLGVERKSRWTYRGEWLGGLKGRSGVWESVSGLRYAGLWKDGFQDGYGTETYSDGGTYQGQWQAGKRHG YGVRQSVPYHQAALLRSPRRTSLDSGHSDPPTPPPPLPLPGDEGGSPASGSRGGFVLAGPGDADGASSRK RTPAAGGFFRRSLLLSGLRAGGRRSSLGSKRGSLRSEVSSEVGSTGPPGSEASGPPIPAPPALIEGSATE VYAGEWRADRRSGYGVSQRSNGLRYEGEWLGNRRHGYGRTTRPDGSREEGKYKRNRLVHGGRVRSLLPLA LRRGKVKEKVDRAVEGARRAVSAARQRQEIAAARAADALLKAVAASSVAEKAVEAARMAKLIAQDLQPML EAPGRRPRQDSGGSDTEPLDEDSPGVYENGLTPSEGSPELPSSPASSHQPWRAPACRSPLPPGGNWGPFS SPKAWPEEWGGPGEQAEELAGYEAEDEAGVQGPGPRDGSPLLGGCSDSSGSLREEEGEDEESLPQLRAPG GSESEPVTTPVLRGLSSRGPDAGCLTEEVEEPAPTERPAQPGAANPLVVGAVALLDLSLAFLFSQLLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052049 |
| ORF Size | 1884 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052049.1 |
| RefSeq Size | 3556 bp |
| RefSeq ORF | 1886 bp |
| Locus ID | 319984 |
| MW | 66 kDa |
| Gene Summary | Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH4 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218681 | Jph4 (untagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347), (10ug) |
CNY 6,940.00 |
|
| MG209581 | Jph4 (tGFP-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347) |
CNY 6,940.00 |
|
| MR209581L3 | Lenti ORF clone of Jph4 (Myc-DDK-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347) |
CNY 8,170.00 |
|
| MR209581L4 | Lenti ORF clone of Jph4 (mGFP-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347) |
CNY 8,170.00 |
