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Pck2 (BC010318) Mouse Tagged ORF Clone
CAT＃: MR209685
- TrueORF®
Pck2 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227)
ORF Plasmid: tGFP
"BC010318" in other vectors (4)
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CNY 4,768.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810010O14Rik; 9130022B02Rik; PEPCK-M |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209685 representing BC010318
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTATGTACCTCCCCGGCCTGCGGCTTAGCCGGCACGGGCTAAGGCCCTGGTGCTGGTCACCGT GCCGTAGCATCCAAACCCTGCGTGTGCTCAGTGGAGATATGAGTCAGCTGCCGGCTGGAGTTCGAGACTT TGTGGCGCGCAGTGCGCATCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGGACCGAGGCTGAGAAC ACTGCCATACTGGCCCTGCTGGAAGAACAGGGTCTTATCCGCAAACTCCCCAAGTATAAGAACTGCTGGC TGGCCCGCACAGACCCCAAGGATGTGGCACGGGTAGAAAGCAAGACGGTGATTGTAACTCCTTCGCAGCG GGACACAGTGCCTCTCCTGGCTGGTGGGGCCAGGGGGCAGCTGGGCAACTGGATGTCCCCAGATGAGTTC CAGAGAGCTGTGGACGAGAGATTCCCAGGATGCATGCAGGGCCGCATTATGTATGTGCTTCCATTCAGCA TGGGTCCCGTGGGCTCCCCACTCTCCCGCATTGGAGTGCAGCTCACTGACTCGGCTTACGTGGTGGCCAG TATGCGTATTATGACCCGCTTGGGGACACCTGTACTTCAGGCCCTGGGAGATGGTGACTTCATCAAGTGT CTGCATTCAGTGGGCCAGCCCCTGACTGGACATGGGGATCCTGTGGGCCAGTGGCCGTGCAATCCAGAAA AAACCCTGATTGGCCACGTGCCAGACCAGCGGGAGATCGTCTCCTTCGGCAGCGGCTATGGTGGTAACTC CTTGCTGGGCAAGAAGTGCTTTGCCCTGCGCATCGCCTCTCGCCTGGCCAGGGATGAGGGCTGGCTGGCA GAGCACATGCTGATTTTGGGCATCACCAACCCTGCAGGGAAAAAGCGCTACGTGGCAGCTGCTTTCCCCA GTGCCTGTGGCAAGACCAATCTGGCCATGATGAGACCTGCATTGCCGGGCTGGAAAGTGGAGTGTGTGGG GGATGACATCGCCTGGATGAGGTTTGACAGTGAAGGTCAACTCCGGGCCATCAACCCTGAGAATGGCTTC TTTGGGGTGGCCCCTGGTACCTCTGCTGCCACCAATCCCAATGCCATGGCCACAATTCAGAGTAATACTC TCTTCACCAACGTGGCTGAGACCAGTGATGGCGGTGTGTACTGGGAAGGCATTGACCAGCCTCTTCCGCC TGGTGTCACCATAACCTCGTGGCTGGGAAAGCCCTGGAAACCTGGTGACAAGGAACCCTGTGCACATCCA AACTCGCGCTTTTGTGTCCCAGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAAGCACCAGAAGGTG TCCCTATTGATGCCATCATCTTTGGAGGCCGCCGACCCAAAGGGGTACCACTGGTGTACGAGGCCTTCAA CTGGCGTCATGGGGTGTTTGTAGGTAGCGCCATGCGCTCTGAGTCCACTGCCGCTGCGGAGCACAAAGGA AAGACCATTATGCATGATCCCTTTGCCATGCGGCCTTTTTTTGGCTATAACTTTGGACGCTACCTGGAAC ACTGGTTGAGCATGGAGGGACAAAAAGGTGCCCGGCTGCCTCGTATCTTCCATGTCAATTGGTTCCGGAG AGATGAAGCAGGCTGCTTCCTGTGGCCAGGCTTTGGAGAGAATGCTCGTGTGCTAGACTGGATCTGCCGA AGATTAGAAGGAGAAGACAGTGCCCAAGAGACTCCCATTGGGCTAGTACCAAAGGAAGGAGCCCTGGACC TCAGTGGCCTCAGCGCAGTGGACACCAGTCAGCTGTTCTCCATCCCCAAGGACTTCTGGGAGCAGGAGGT TCGTGATATTCGGGGCTACCTGACAGAGCAAGTCAACCAGGACCTGCCCAAGGAGGTGTTGGCTGAGCTC GAGGCTCTGGAAGGACGTGTACAAAAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209685 representing BC010318
Red=Cloning site Green=Tags(s) MAAMYLPGLRLSRHGLRPWCWSPCRSIQTLRVLSGDMSQLPAGVRDFVARSAHLCQPEGIHICDGTEAEN TAILALLEEQGLIRKLPKYKNCWLARTDPKDVARVESKTVIVTPSQRDTVPLLAGGARGQLGNWMSPDEF QRAVDERFPGCMQGRIMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFIKC LHSVGQPLTGHGDPVGQWPCNPEKTLIGHVPDQREIVSFGSGYGGNSLLGKKCFALRIASRLARDEGWLA EHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGQLRAINPENGF FGVAPGTSAATNPNAMATIQSNTLFTNVAETSDGGVYWEGIDQPLPPGVTITSWLGKPWKPGDKEPCAHP NSRFCVPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKG KTIMHDPFAMRPFFGYNFGRYLEHWLSMEGQKGARLPRIFHVNWFRRDEAGCFLWPGFGENARVLDWICR RLEGEDSAQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPKEVLAEL EALEGRVQKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010318 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010318.1 |
| RefSeq Size | 2143 bp |
| RefSeq ORF | 1922 bp |
| Locus ID | 74551 |
| MW | 78.5 kDa |
| Gene Summary | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200688 | Pck2 (untagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227), (10ug) |
CNY 4,776.00 |
|
| MG209685 | Pck2 (tGFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227) |
CNY 3,990.00 |
|
| MR209685L3 | Lenti ORF clone of Pck2 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227) |
CNY 5,510.00 |
|
| MR209685L4 | Lenti ORF clone of Pck2 (mGFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227) |
CNY 5,510.00 |
